2 micron plasmid extraction

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2 micron plasmid extraction

by Ellie Harrison :: Rate this Message:

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Hi all,

I'm looking to extract naturally occurring 2 micron plasmid from yeast.  There seem to be a range of plasmid DNA preps but having tried the qiagen plasmid kit and failed i would appreciate a recommendation before buying into different kits.

Does anyone have any experience with this?

All help much appreciated

Ellie

PS. thanks for all replies regarding aAA selection- please feel free to contact me if interested in the results!