Advice on mammalian cells

[Martin Oneil]

We would like to do YFP localization and BiFC assays in a mammalian cell line. Does anyone have any recommendations on with cell lines to buy that are easy to transfect and image using confocal microscopy?

Thank you in advance for your time


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[WS-4]

Hi,

in my experience, 3T3 mouse fibroblasts are easy to culture, easy to
transfect and give nice pictures. They should be easy to obtain, maybe
you even know a lab doing cell culture, they might have them.

Wo
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