Nabble - Bio.net - Biofilms

biofilm question

2009-10-05T12:23:39Z

Hello, I am a Biofilms mailing list membership, I want to know the Agar red Congo composition , I want to study the biofilm phenotypic expression in Satphylococcus aureus.

Best regards

Lic. Joan Peña Rodríguez
Microbiology Laboratory, CENLAC
National Center for Animal and Plant Health (CENSA)
Po Box 10, San José de las Lajas, CP 32700
Telef: 5347-863014, ext 24, 5347-863145
Fax: 5347-868104, 5347-863897
Havana, Cuba

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Request for information

2009-09-24T04:18:47Z

Hi All,

I am new member to this forum. Let me introduce myself. I am Yogesh Joshi from University of Salerno,Italy. Currently I am doing research on single species biofilm (Kluyveromyces marxianus) and its application in fermentation. Anyone working on this subject or can anyone provide the email ids for the people who are working.

Regards

Yogesh

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Hello

2009-08-08T05:44:46Z

Prem Saran mailing ..

Is there a standard procedure for Growth of Biofilms Invitro conditions ?? Also on Biofilm assays .

Thanx

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Removal of biofilms from tubes

2009-06-16T02:54:29Z

Dear all,

I'm looking for the best way to safely remove biofilms from tubes. The tubes are made of PE (poly-urethane).

I've seen several reports on sonication for the removal, but in those reports the material is always steel or iron. I'm wondering if the sonication procedure should change if the material is different.

The biofilms are being removed to perform a quantative pcr on it. (real-time 16s PCR)

Thank you very much,

Dhr. P.M. ter Huurne

Deltares / TNO

Subsurface and Groundwater Systems

Geosciences laboratories

Princetonlaan 6

P.O. Box 85467

3508 AL Utrecht

+31 (0)623371484

paul.terhuurne@...

www.deltares.nl

www.tno.nl

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RE: pseudomonas aeruginosa biofilm formation in lab

2009-02-09T08:49:00Z

I recommend you contact the Center for Biofilm Engineering at Montana State University. They have a number of helpful materials and resources to help you get started.

Best regards,

Diane Gray

Diane R. Gray
Research Scientist
Cellular Systems
Life Technologies

29851 Willow Creek Rd
Eugene, OR 97402
T 541.465.8300 x59754
F 541.335.0177

Diane.Gray@...
www.lifetechnologies.com

From: biofilms-bounces@... [mailto:biofilms-bounces@...] On Behalf Of Harini Swaminathan
Sent: Saturday, February 07, 2009 6:40 AM
To: biofilms@...
Subject: [Biofilms] pseudomonas aeruginosa biofilm formation in lab

helo frnz,

first of all, i wud lik to thank d moderator of ds grp fr havin a discussion forum on Biofilms.. I am new to this group.. i ve jus subscribed.. i am studyin in chennai.. intrstd to do proj in pseudomonas aeruginosa biofilms.. i ve onl basic knowledge abt wat biofilm is.. so pls help me in carryin out this proj.. i wanted to knw as how p.aeruginosa can be grown as a biofilm in laboratory condition.. is it possible 2 get a large sample by growin d culture under batch conditions instead of growin in continuous tubing reactor as we dont hav that set up facilities in our lab.. if so can u suggest me d expt methods fr biofilm formation & screening.. i wanted to work on biofilm associated proteins, its structural characterisation.. m concentrating in proteomics to study by 2D- PAGE.. pls reply me ur valuable suggestions.. Thank u..

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pseudomonas aeruginosa biofilm formation in lab

2009-02-07T06:40:29Z

TRFLP on biofilms

2008-11-26T22:30:11Z

TRFLP on biofilms

Hello,

I am looking for a protocol for trflp on marine biofilms. hope someone could help me.

V


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Looking for a reference for one of your suggested protocols

2008-10-23T09:19:47Z

Hello Mr. Wright,

I'll begin by apologizing if I have addressed you incorrectly, I came across your name in a rather dated (1997) correspondence with George O'toole when searching for a good way of enumerating the viable cells in a biofilm. The method you describe to Mr. O'toole (employing sonication of cells followeed by dilution) sounds perfect my intended application;enumerating the E. coli cells from a biofilm in a microplate, and was hoping that you may have on hand a reference for the protocol you described. Here is a link to the description you provided http://www.bio.net/bionet/mm/biofilms/1997-April/000106.html. I realize this is a very long shot but figured I would try none-the-less. Thank you very kindly in advance.

Sincerely,

Stefan Moreira H.B.Sc.Bio.
M.Sc. Candidate
Lakehead University
Thunder Bay, ON
807-252-3860

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Re: Journals in the field

2008-09-18T06:05:06Z

Hi,

You can search in Pubmed: www.pubmed.org

Cheers,

Tanvir

On Sep 15, 4:52 pm, srijan aggarwal <srijan.aggar...@...> wrote:
> Dear All,
>
> I am working on cohesive strength of biofilims. Can anyone provide me
> a comprehensive list of journals related to this field, which may be
> useful for this area. And as I am realizing, it is very diverse so
> thought of taking help of you all !
>
> Srijan Aggarwal

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bacterial surface depostion-Flow cell

2008-09-17T02:11:08Z

Hi all,

thank you for your suggestions in advance

I am working on the effect of some chemicals on bacterial depostion on Reverse Osmosis Membranes using a flowcell under a fluorescent microscope. But, I have a problem in counting the bacteria deposited on the membrane in both cases (control and chemicals).

What factors do I have to take in to consideration?

Thanks,

Merhawi

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Journals in the field

2008-09-15T09:52:26Z

Dear All,

I am working on cohesive strength of biofilims. Can anyone provide me
a comprehensive list of journals related to this field, which may be
useful for this area. And as I am realizing, it is very diverse so
thought of taking help of you all !

Srijan Aggarwal

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StatorSystems

2008-07-31T02:59:34Z

Can anyone tell me where MotX and MotY are localized in the sodium-dependent
statorsystem? in the outer membran (Fukuoka 2006) or inner membran
(McCarter 1994)?
or could it be that it is difuse localized in periplasmatic room, without
membran binding (Kojima 2008)?

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Help-measurement of Bulk Modulus of Biofilm

2008-06-23T07:32:38Z

Dear all,

I am looking for an standard method to measure Bulk Modulus of Biofilm. I did not find any method in the previous literature regarding this issue..

Would you please suugest whatever you think would be useful for this type of compression test (in hydrostatic stress condition)?

The initial test results suggests to me that biofilm can be treated as a nearly incompressible material (Poisson's ratio of 0.49 N/m^2), this is assumed by some other people (0.5) and others assumed 0.3 which suggest that biofiulm is compressible...I am not clear what value I should use for simulation

Many Thanls,

--
Ashkan

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Biofilms Training Course in Denmark Aug 18.-25.

2008-05-13T01:06:24Z

Dear All,

We are giving a biofilms training course in Denmark in August (18^th-25^th). The course is open to all, and if you are a PhD student you can earn 5 ECTS points. Please see details on our course description page, and on the enclosed poster, which I will encourage you to print out and place on a noticeboard in your institution.

Thanks,

Claus Sternberg

Link to course description:

http://www.kurser.dtu.dk/27820.aspx?menulanguage=en-gb (NB: this year 8 days incl the weekend).

Claus Sternberg Assoc. Prof., Ph.D. BioSys-DTU Infection Microbiology Group Building 301 Kgs. Lyngby, DK- 2800 Denmark		Work: 45 45252770 Fax: 45 45932809 Email: cst@... IM: claussternberg (Skype)

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BiofilmTechniques-2008.pdf (797K) Download Attachment

59th Annual International Science and Engineering Fair - Call for Judges

2008-03-31T11:01:45Z

The 59th ISEF will be held this year in Atlanta, Georgia May
11th-17th. I am looking for volunteers to serve as Grand Awards
Judges in the Microbiology Category. Last year's ISEF in Albuquerque,
New Mexico, over 45 different countries sent 1510 secondary school
finalists to compete. We expect the same turn out at this year's
Fair. I am looking for judges to choose the top finalists who will
compete for thousands of dollars in prizes and awards. Grand Awards
Judges will arrive in Atlanta on Tuesday, May 13 for orientation and
pre-judging and will judge on Wednesday, May 14. This is the premier
worldwide science research competition for high school students grades
9-12 and we need your support. If you are interested in serving as a
judge at this year's ISEF, please go to the following website and
register:

http://www.intelisef2008.org/judging.html

If you need more information, don't hesitate to contact me at
drkjroberts@...

Karl J. Roberts, Ph.D.
Co-Chair, Microbiology Division
International Science and Engineering Fair

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RNA isolation from biofilm

2008-03-13T08:52:44Z

Dear all:

What's the best way to isolate the RNA from biofilm
for RT PCR.

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compression test on biofilm

2008-03-11T09:14:32Z

Dear all,

I am a mechanical engineer who is measuring biofilm mechanical properties. I am currently doing compression tests on mixed biofilm. I have two questions:

Biofilm used in the test is a few weeks old, do I expect to see big difference in results when I do it on very fresh biofilm?
I had a 100N load cell and I know sensitivity is low, does it affect results in unacceptable way?
Biofilm is placed in a glass container and I have a surface tension problem and biofilm sticks to the glass wall, any recommendation to reduce adhesion? I used PTFE lubricant but didn’t work well.

Thanks in advance,

Ashkan Safari

PhD Research Student

UCD School of Electrical, Electronic and Mechanical Engineering

Room 314

University College Dublin

Belfield

Dublin 4

Ireland

Ph: +353 1 716 1880

Mob: +353 860867511

E-mail: Ashkan.Safari@...

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Thank you for your information on biofilm questins but i have more questions

2008-03-11T01:40:45Z

Thank you so much for your brief information on the previous biofilm questions. I also have another question.

Q. usually, procedures followed in the lab for bacterial surface analysis are destructive to the bacterial surface composition which might ofcourse lead to wrong conclusion.

- Washing procedure

- Type of washing and resuspension medium used

- Speed of centrifugation

Thus, does any body have any idea on how to handle these procedures so that to minimize the damage?

thank you so much agian.

tesfalem

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Today's Topics:

1. Re: Biofilm experiment questions (bob)

----------------------------------------------------------------------

Message: 1
Date: Tue, 04 Mar 2008 20:20:22 -00800
From: bob
Subject: [Biofilms] Re: Biofilm experiment questions
To: bionet-microbiology-biofilms@...
Message-ID:
Content-Type: text/plain; charset=ISO-8859-1

On Sun, 2 Mar 2008 05:09:08 -0800 (PST), merhawi debesai
wrote:

>Hi,
> Thank you so much for your information in advance.
> Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions:
>

> 2. The second question is regarding the preparation of bacterial strains for
> surface analysis.
> i. Usually, we prepare the test strains in suspensions. However, it is
> known that there....difference between planktonic and sessile (biofilm)
> bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?!

I think you just answered your own question.

Not sure if they make a different EPS when sessile, rather than just
more of it. But seems to me you would want to at least check.

Once you get your methodology running, try comparing.

By the way, there is a nice overview article in February issue of
Microbe:

How Pseudomonas aeruginosa Regulates Surface Behaviors
George A. OToole
At surfaces, these bacteria either form biofilms or swarm, a
regulated behavior with
important consequences for pathogenesis
http://www.asm.org/microbe/index.asp?bid=55982

bob

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Re: Biofilm experiment questions

2008-03-04T20:20:22Z

On Sun, 2 Mar 2008 05:09:08 -0800 (PST), merhawi debesai
<samiboy_4ever@...> wrote:

>Hi,
> Thank you so much for your information in advance.
> Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions:
>

> 2. The second question is regarding the preparation of bacterial strains for
> surface analysis.
> i. Usually, we prepare the test strains in suspensions. However, it is
> known that there....difference between planktonic and sessile (biofilm)
> bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?!

I think you just answered your own question.

Not sure if they make a different EPS when sessile, rather than just
more of it. But seems to me you would want to at least check.

Once you get your methodology running, try comparing.

By the way, there is a nice overview article in February issue of
Microbe:

How Pseudomonas aeruginosa Regulates Surface Behaviors
George A. OToole
At surfaces, these bacteria either form biofilms or swarm, a
regulated behavior with
important consequences for pathogenesis
http://www.asm.org/microbe/index.asp?bid=55982

bob

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Biofilm experiment questions

2008-03-02T05:09:08Z

Hi,

Thank you so much for your information in advance.

Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions:

1. On QCM-D data interpretation:

i. frequency change --> adsorbed mass

ii. energy of dissipation --> to visico-elastic property of the adsorbed

mass.

2. The second question is regarding the preparation of bacterial strains for

surface analysis.

i. Usually, we prepare the test strains in suspensions. However, it is

known that there....difference between planktonic and sessile (biofilm)

bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?!

Sincerly yours,

tesfalem

fish for candida biofilms

2008-02-24T06:32:21Z

can any body suggest me a FISH technique for candida biofilms
--
Regards
T.S.Subha
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BCBGC-08 Final call for papers

2008-01-23T10:18:58Z

BCBGC-08 Final call for papers

The 2008 International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-08) (website: www.PromoteResearch.org ) will be held during July 7-10 2008 in Orlando, FL, USA. The draft paper submission deadline is February 4 2008.

BCBGC brings together both academic and industrial scientists and developers from a diverse range of disciplines including bioinformatics, computer science, computational biology, genomics, proteomics and chemoinformatics. One of the main goals of the conference is to promote the dissemination of research to a multidisciplinary audience and to facilitate communication among researchers in different fields. Papers that demonstrate applications of existing techniques or developments of new methods are equally welcomed to the conference. The conference will be held at the same time and location where some other major events will be taking place. The website contains more details.

Sincerely

John Edward

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PM Newsletter

2007-09-20T11:02:44Z



	PM Technology Description/Overview

	Drug Discovery Using PMs

	PMs for Microbial Cells

	PMs for Mammalian Cells

	Publications on PM Technology

	Phenotype MicroArray Services

	Access to PM Technology

Bacillus subtilis Metabolism and Gene Regulation Studied in Two Recent Applications of Phenotype MicroArray Technology

Genome-scale reconstruction of metabolic network in bacillus subtilis based on high-throughput phenotyping and gene essentiality data. Oh YK, Palsson BO, Park SM, Schilling CH, Mahadevan R. Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S3E5. This landmark paper is the first example of a careful and thorough use of Phenotype MicroArray data to challenge and improve a whole-cell bioinformatics model of Bacilllus subtilis. Network gap analysis was used to identify 48 essential reactions missing from the genome annotation. Detailed growth rate analysis revealed incorrect modeling outcomes which were subsequently improved by adding 75 reactions. Simulation of growth phenotypes of knock-out strains were verified in 720 of 766 cases. Overall, the integrated analysis of substrate utilization and gene essentiality data led to identification of 80 required enzymes and improved the genome annotation for Bacillus subtilis.

Regulatory Overlap and Functional Redundancy Among Bacillus subtilis Extracytoplasmic Function (ECF) (sigma)-Factors. Mascher T, Hachmann AB, Helmann JD. Department of Microbiology, Cornell University, Ithaca, NY 14853-8101, USA. The biological role of extracytoplasmic function sigma factors in Bacillus subtilis was examined in this paper. Seven genes encode these sigma factors, so the authors constructed strains with multiple gene knockouts and then used Phenotype MicroArray technology to test the phenotypes of their strains. A triple mutant in sigM, sigW, and sigX exhibited greatly increased sensitivity to detergents, polymyxin B, B-lactams, and D-cycloserine.

Printable PDF

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For more information on PM technology
For information on Biolog's Phenotype MicroArray Services
To view Biolog's PM publications and bibliography
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"Forward-to-a-friend" link can be found at the bottom of the page.

Brief description of PM technology

Phenotype MicroArray™ technology tests cellular phenotypes. Included in the tests are assays of basic cellular nutritional pathways for C, N, P, and S metabolism, osmotic and pH and sensitivity, and sensitivity to chemical agents.

The most common application is to assess the phenotypic effects of mutations. A change in genotype of a cell should lead to one or more changes in phenotype. PMs allow testing of knock-out or knock-in mutants to help discern the biological changes that occur consequent to genetic changes.

Another common application is phenotypic characterization of a collection of related strains. For example, it is possible to determine the phenotypic relatedness of a collection of isolates of a given species. PM analysis has been successfully implemented for a wide variety of model microbial cells including Escherichia coli,

Salmonella enterica, Pseudomonas aeruginosa, Pseudomonas putida, Sinorhizobium meliloti, Saccharomyces cerevisiae, Bacillus subtilis, Bacillus cereus, Shewanella oneidensis, Proteus mirabilis, and many other species. In the last year, Biolog has successfully developed universal protocols that allow us to test most species of interest. Contact us for details.

Phenotype MicroArray™ technology uses Biolog's OmniLog® instrument, which is also used for microbial identification. The OmniLog® automatically incubates, reads and interprets the Biolog Phenotype MicroArray MicroPlates™. It continuously processes samples but allows

the user complete access at any time during a sample run. Samples can be loaded when ready and removed when complete without disturbing other samples still in-process. Inside the Reader there are 25 trays. Each tray holds 2 MicroPlates, giving the Reader a total capacity to incubate and read 50 MicroPlates. Before the user inoculates the appropriate MicroPlates, they log the MicroPlate information into the OmniLog® software.

By simply following the software's instructions, the user then opens the door of the OmniLog® Reader and places each MicroPlate in the appropriate tray slot indicated by the software. Once all the MicroPlates are loaded and the door of the Reader is closed, the OmniLog® software takes over the responsibility for incubating, reading, saving, and printing the results. The OmniLog® PM System utilizes Windows-based software with an elegantly simple user interface. The status of all the samples can be observed by simply looking at the Read menu screen of the OmniLog® PM System software. All of the information for a specific MicroPlate is contained on a single line of this screen. Once the system has completed a specific MicroPlate it indicates this status with a check mark icon. A clock icon is used for those samples that are still incubating

because a result has not yet been determined. The OmniLog® PM System's software is extremely flexible and can be integrated into virtually any laboratory's workflow. New MicroPlates can be entered into the Reader and MicroPlates that have already completed can be removed from the Reader at almost any time. This allows the user to either perform experiments one at a time or batch them.

PM Technology and the OmniLog® instrument are ideal for a core facility or for purchase under a shared equipment grant.

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Invitation to submit abstract(s) to Applied Microbiology 2007

2007-06-21T19:07:54Z

II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007), Seville (Spain), 28 November - 1 December 2007

http://www.formatex.org/biomicroworld2007

Abstracts deadline: 17 September / Early Registration deadline: 22 August

Dear colleague,

On behalf the organisers, you are cordially invited to send abstracts of your best research for presentation at the forthcoming II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007), to be held in Seville (Spain) from 28 November to 1 December 2007. All the information regarding this interdisciplinary conference can be found at the conference website: http://www.formatex.org/biomicroworld2007

Modern microbiology includes a broad variety of scholarly approaches leading to a better understanding of all living things at the macroscopic, microscopic/single-cell and nanoscopic/molecular level, producing beneficial applications in medicine, agriculture, industry, and ecology.

The Conference will specially welcome papers reporting interdisciplinary researchers, relating microbiology with other sciences as physics/chemistry, environmental sciences, materials science, polymer science, genetics, pharmacology, microscopy/imaging science, etc. In other words, we are specially (but not exclusively) interested in papers that relate the techniques and culture of microbiology to research areas usually associated with other scientific and engineering disciplines.

GENERAL TOPICS OF BIOMICROWORLD2007

Space Microbiology, Astro(micro)biology
Agriculture, Soil, Forest Microbiology
Analytical Techniques, Imaging Techniques, Microscopy
Cell Engineering
Environmental Microbiology, Marine Microbiology, Water/Aquatic Microbiology, Geomicrobiology
Food Microbiology
Industrial Microbiology - Future Bioindustries
Medical Microbiology
Methods in Basic and Applied Microbiology
Microbial Biotechnology
Microbial Physiology, Metabolism and Gene Expression
Microbiology Education
Pharmaceutical Microbiology
Structure and Morphogenesis
Quantitative Models and Bioinformatics in Microbiology

IMPORTANT DATES

Abstract submission deadline: 17 September 2007

Early registration deadline: 22 August 2007

Final registration deadline: 21 November 2007

Full paper submission for publication: On-site (28 November 1 December)

CONFERENCE PUBLICATIONS

Proceedings Book

A multi-volume book will be published including full papers of works (oral, posters and virtual posters) presented at the Conference. The book will be published by an international publisher in order to give it a broad international distribution and to serve as a good reference to consult the state of the art of the hottest topics in Modern Microbiology. The paper length will be 5 printed pages. Submission of full papers is optional.
Abstract Book
A separate Abstracts book will be published with abstracts of works presented at the Conference. It will be distributed among full rate participants at the beginning of the Conference, and dispatched to virtual participants after it.
International Journals special issues
Agreements are being negotiated with several international journals, in order to produce special issues based on very good papers presented at the Conference. Manuscripts must be delivered during the Conference, according to the instructions that we will give in due course for each journal. All papers will be strictly reviewed and treated as regular papers. Our goal is to produce high quality and high impact special issues based on some of the best conference papers. Papers submitted for a journal special issue and rejected (for scientific/editorial/space reasons), can be considered for inclusion in the Proceedings book. Please refer to the conference website for details.
If you would like to know more information about the Proceedings Book and the International Journals special issues published after the 1st edition of this conference, BioMicroWorld2005 held during 15-18 March 2005, click here

VIRTUAL PARTICIPATION

In addition to the "traditional" oral contributions and posters presentations, a Virtual Participation modality has been established for those researchers unable to attend the Conference personally. Please consult the conference website: http://www.formatex.org/biomicroworld2007/virtual/

COLLABORATION PROJECTS: SEARCH FOR PARTNERS

BioMicroWorld2007 will also serve as a platform for partners search for trans-national collaboration projects, like for example the EU Seventh Framework Program (Networks of Excellence, Integrated Projects and Specific Targeted Research or Innovation Project). All ideas/proposals for collaboration will be included in a booklet which will be distributed at the beginning of the Conference and/or posted at the conference website. If you are interested in taking part, please send us the corresponding form available at the website.

RESEARCH GROUPS

Send details of your group activities (research topics, members, projects, publications...). The booklet with all participating groups will be distributed at the Conference and/or posted at the conference website.

For any questions or suggestions, please do not hesitate to contact us at biomicro2007@...
We would also appreciate if could disseminate this Call for Papers through your Department or Institution.
We hope to meet you at this large interdisciplinary international meeting!

Ms Aurora Solano
BioMicroWorld2007 Secretariat (on behalf of Mr Antonio Mendez-Vilas, Conference Chairman)

FORMATEX Research Center
C/ ZurbarАn 1, 2nd Floor
06002 Badajoz, SPAIN
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http://www.formatex.org/biomicroworld2007
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antibiotic sensitivity of biofilms

2007-06-21T08:52:41Z

hello everyone!

May be I am asking a very simple and naive question, but I have a
doubt regarding the terms we use for checking antibiotic sensitivity
of biofilms. For checking antibiotic sensitivity of planktonic cells,
we use the terms MIC (minimum inhibitory concentration) and MBC
(minimum bactericidal concentration). Can we apply the same terms for
biofilms also? Or do we use the terms MBPC (minimum biofilm preventive
concentration) and MBEC (minimum biofilm eradication concentration) as
markers for antibiotic sensitivity of biofilms?

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Re: staining of bacterial biofilms

2007-06-20T06:07:34Z

On Jun 20, 12:48 am, Bob McLean <mcl...@...> wrote:

> Dear Rachna,
>
> While in theory any stain that would stain biological components of
> biofilms (and not the underlying polystyrene substratum) could be
> used, crystal violet absorbs much more light than safranin and so
> would provide higher sensitivity for this assay.
>
> Good luck with your work,
>
> Bob McLean
>
> At 09:15 AM 6/19/2007, Rachna Singh wrote:
>
> >Why is crystal violet preferred over safranin for staining bacterial
> >biofilms formed on the surface of microtiter plates?
>
> >_______________________________________________
> >Biofilms mailing list
> >Biofi...@...
> >http://www.bio.net/biomail/listinfo/biofilms
>
> R.J.C. (Bob) McLean
> Department of Biology
> Texas State University
> 601 University Drive
> San Marcos, TX 78666
> USA
>
> New University Name: Texas State University
>
> (512) 245-3365 phone
> (512) 245-8713 FAX
> Email: McL...@...
>
> Website:http://www.bio.txstate.edu/~micro/mclean/mclean.html

OK. Thanks a lot for your help.

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Re: staining of bacterial biofilms

2007-06-19T13:48:29Z

Dear Rachna,

While in theory any stain that would stain biological components of
biofilms (and not the underlying polystyrene substratum) could be
used, crystal violet absorbs much more light than safranin and so
would provide higher sensitivity for this assay.

Good luck with your work,

Bob McLean

At 09:15 AM 6/19/2007, Rachna Singh wrote:
>Why is crystal violet preferred over safranin for staining bacterial
>biofilms formed on the surface of microtiter plates?
>
>_______________________________________________
>Biofilms mailing list
>Biofilms@...
>http://www.bio.net/biomail/listinfo/biofilms

R.J.C. (Bob) McLean
Department of Biology
Texas State University
601 University Drive
San Marcos, TX 78666
USA

New University Name: Texas State University

(512) 245-3365 phone
(512) 245-8713 FAX
Email: McLean@...

Website: http://www.bio.txstate.edu/~micro/mclean/mclean.html

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Biofilm model

2007-06-19T08:41:34Z

Hello everyone!

Out of microtiter plate and colony biofilm model, which one is better
for growth of biofilms? Can anyone please let me know the relative
advantages and disadvantages of both these models?

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staining of bacterial biofilms

2007-06-19T08:15:23Z

Why is crystal violet preferred over safranin for staining bacterial
biofilms formed on the surface of microtiter plates?

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Re: (no subject)

2007-06-16T05:45:09Z

Dear Indra:

I suggest you to visit few of these webpages for understanding the
Instructions to authors and few more guidelines that seems to be
important when sending a paper.

Biofilm journal is infact a good journal and one of the best in the
field. I would like you to visit the following webpages:
1. http://www.journals.cambridge.org/action/displayJournal?jid=BFM
2. http://www.erc.montana.edu/Res-Lib99-SW/newsarchives/Biofilms%20Journal/default.htm
3. http://www.journals.cambridge.org/action/displayMoreInfo?jid=BFM&type=ifc

The impact factor of this journal is around 1.0.

REgards,
Devendra

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(no subject)

2007-06-13T15:41:13Z

Dear all,

I want to publish a paper in Biofilm journal. What's
the impact factor of this journal and how I can
request for submission (Review)

Thanks

____________________________________________________________________________________
Sick sense of humor? Visit Yahoo! TV's
Comedy with an Edge to see what's on, when.
http://tv.yahoo.com/collections/222

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International Biodeterioration Society Meeting&In-Reply-To=

2007-04-20T03:53:12Z

Hello

At the moment I am arranging an Emeritus Professor Award to Professor Glyn Morton from the University of Central Lancashire in Preston on the 5th June 2007.

I am trying to get hold of the address of Ms Diane Band with whom Glyn has been a colleague and who is a member of the Biodeterioration Society. I wonder if you have her address or email address so that I can forward a personal invitation to her?

Thank you. Gillian Saunders

gsaunders@...

Gillian Saunders
Faculty of Science and Technology
University of Central Lancashire
Maudland Building
Preston
PR1 2HE

Tel: 01772 893478
Fax: 01772 892903

Any representations, views and/or opinions in this e-mail are those of the sender, and not necessarily of the University. The University's computer network (including its e-mail facility) is intended for educational purposes, and the University accepts no liability for, and shall not be bound by, the contents of any e-mail sent from its network which do not relate to those purposes or other business of the University.
This e-mail is confidential to and for the sole use of its addressee(s). If you should not have received it, please notify the sender and permanently delete it: you may not print, copy, disclose, distribute or rely on it, or any part of it.
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IWA Biofilm Technologies conference_Call for papers

2007-03-08T20:11:16Z

Dear colleagues,

The International Water Association, in cooperation with the IWA Biofilm Group, Nanyang Technological University and National University of Singapore, is pleased to invite you to attend IWA BIOFILM TECHNOLOGIES conference.

Don't miss your opportunity to dialogue and network at Biofilm Technologies conference on 8 - 10 January, 2008 in Singapore. This conference is of the IWA Biofilm Conference Series and serves to discuss scientific and practical aspects of cell aggregation and the processes, where aggregated microbial cells play a major role. This covers biofilm–based technologies and reactors, biofilm processes, biofilm microbiology, biofouling and biocorrosion caused by biofilms. The topics of the conference will be as follows:

· Biofilm microbiology and ecology

Molecular biology of biofilms
Public health and biosafety of microbial biofilms
Marine biofilms
Ships hull biofilms
Innovative environmental engineering applications of biofilms
Membrane-associated biofilm processes
Water treatment using biofilms Wastewater treatment using biofilms
Biofilms in groundwater and soil bioremediation
Biofilm treatment of gases
Modeling, design, and applications of biofilm reactors
Pilot and full scale biofilm-base processes
Anaerobic biofilms
Microbial granules
Biofouling, biocorrosion, and biodeterioration caused by biofilms
Biofilms in biosensors and microfluidics
Bioclogging and biocementation in porous media

Scientific papers as well as well documented case studies are all encouraged for submission.

ABSTRACT SUBMISSION DEADLINE: April 30, 2007
Submit your 2-pages abstract before April 30, 2007 the Conference Secretariat attached to an email as a Word Document or PDF: http://www.biofilmtechnologies.com/abstracts.asp

or you may to download this form as a Word Document from https://www.orient-explorer.com/biofilm/BiofilmTechnologiesRegForm.doc and send it by fax.

Save from 10% to 20% due to registration before 15 August 2007!

Contact: Marianne Yee
Biofilm Technologies conference secretariat
c/o Orient Explorer (S) Pte Ltd, 12 Aljunied Road #05-02 SCN Centre, Singapore 389801
Tel: +65 6339 8687 Fax: +65 6339 9536

Email: biofilm2008@...
Website: www.biofilmtechnologies.com

and/or
Dr. Volodymyr Ivanov (Chairman), School of Civil and Environmental Engineering,
Nanyang Technological University, Block N1, 50 Nanyang Avenue, Singapore 639798.
Tel: +65 6790 6934 Fax: +65 6791 0676 Email: cvivanov@...

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Re: Surface energy - meaning?

2006-11-30T22:33:15Z

Surface energy is a measure of disruption of chemical bonds that occurs
when a surface is created. Most of the hydrophobic surfaces are known
to show low surface energy values. It is also well known that
hydrophobicity has a role in cell attachment to the surface and biofilm
formation. As the biofilm progresses the adhesion is higher and
chemical bonding is more hence lower the surface energy.

Few studies were carried out on adhesion of biological systems such as
proteins, microbes, algae and invertebrates. They all show minimal
long-term adhesion associated with surfaces having low energy surfaces.
However, all surfaces rapidly become modified on immersion in natural
waters through the adsorption of conditioning films, which may
influence subsequent adhesive events associated with the permanent
attachment of organisms. The results show that surface energy can
elicit different responses in different organisms. For most organisms,
minimal adhesion is associated with low surface energy.

According to thermodynamics, matter seeks to be in a low-energy state,
and bonding reduces chemical energy. Young's Modulus says, if the
surface is hydrophobic then the contact angle of a drop of water will
be larger. Hydrophilicity is indicated by smaller contact angles and
higher surface energy. Water has high surface energy by nature; it's
polar and forms hydrogen bonds. Hydrophilicity is generally indicated
by smaller contact angles and higher surface energy. Water has high
surface energy by nature; it's polar and forms hydrogen bonds.

Also check : The influence of low surface energy materials on
bioadhesion : a review by Callow M.E.and Fletcher R. L. Int.
biodeterior. biodegrad. International symposium on marine biofouling
and corrosion, Portsmouth, ROYAUME-UNI. 1994, vol. 34, 3-4, pp. 201-412

Best Regards,

Devendra Dusane
--------------------------------------------
Junior Research Fellow,
University of Pune-Bhabha Atomic Research Centre (BARC) programme
Institute of Bioinformatics & Biotechnology (IBB)
University of Pune
Ganeshkhind, Pune-411 007
India
---------------------------------------------
URL: http://devendradusane.tripod.com
email: microbesinaction@...
devendra@...
----------------------------------------------

On Nov 30, 2:18 pm, John Brooks <brook...@...> wrote:

> Have a look at Wendy C. Duncan-Hewitt's paper "Nature of the hydrophobic
> effect" in Microbial Cell Surface Hydrophobicity, Doyle, R.J. &
> Rosenberg, M. (Eds). 1990 Am. Soc. Microbiol.
>
> It's not easy going, but explained a lot for me.
>
> Regards
> John
>
> Alfred Ogola Okello wrote:
> > Dear Sir,
>
> > Ref:http://www.bio.net/bionet/mm/biofilms/1998-September/000364.html
>
> > I am having trouble understanding the concept of surface energy (SE).
> > SE seems to mean the same thing as bonding potentiel.
> > What confuses me is that while hydrophobicity increases as SE
> > decreases, adhesion seems to increases. Why does adhesion increase
> > when the bonding potentiel (SE)decreases. I thought that the trend
> > should be the reverse. Moreover, I think that it contradicts the
> > hydrophobicity theory: adhesion of water decreases as the SE decreases.
>
> > Please make it clear for me; maybe I am missing out on something.
>
> > Best regards,
>
> > Alfred
>
> > ------------------------------------------------------------------------
> > Everyone is raving about the all-new Yahoo! Mail beta.
> > <http://us.rd.yahoo.com/evt=42297/*http://advision.webevents.yahoo.com...>
>
> > ------------------------------------------------------------------------
>
> > _______________________________________________
> > Biofilms mailing list
> > Biofi...@...
> >http://www.bio.net/biomail/listinfo/biofilms
>
> brooksjd.vcf
> 1KDownload

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Re: Surface energy - meaning?

2006-11-30T01:18:55Z

Have a look at Wendy C. Duncan-Hewitt's paper "Nature of the hydrophobic
effect" in Microbial Cell Surface Hydrophobicity, Doyle, R.J. &
Rosenberg, M. (Eds). 1990 Am. Soc. Microbiol.

It's not easy going, but explained a lot for me.

Regards
John

Alfred Ogola Okello wrote:

> Dear Sir,
>
> Ref: http://www.bio.net/bionet/mm/biofilms/1998-September/000364.html
>
> I am having trouble understanding the concept of surface energy (SE).
> SE seems to mean the same thing as bonding potentiel.
> What confuses me is that while hydrophobicity increases as SE
> decreases, adhesion seems to increases. Why does adhesion increase
> when the bonding potentiel (SE)decreases. I thought that the trend
> should be the reverse. Moreover, I think that it contradicts the
> hydrophobicity theory: adhesion of water decreases as the SE decreases.
>
> Please make it clear for me; maybe I am missing out on something.
>
> Best regards,
>
> Alfred
>
> ------------------------------------------------------------------------
> Everyone is raving about the all-new Yahoo! Mail beta.
> <http://us.rd.yahoo.com/evt=42297/*http://advision.webevents.yahoo.com/mailbeta>
>
> ------------------------------------------------------------------------
>
> _______________________________________________
> Biofilms mailing list
> Biofilms@...
> http://www.bio.net/biomail/listinfo/biofilms

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