Nabble - Bio.net - Cellbiol

Most cited research reagents and kits

2009-11-25T08:56:47Z

An interactive graph showing most cited research reagents and kits that are commercially available. The data is based on recent published journal articles, patents and patent applications. The current research reagent/kits listings are Transfection reagents, RT-PCR, Real-time PCR, RNA purification, cAMP assays, Cytotoxicity assays and Western blot reagents. Please visit: http://www.sciclips.com/sciclips/most-cited-reagents.do?cat=TransfectionReagents&catId=1501
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Request For Help in my project

2009-11-12T02:16:33Z

Dear Sir/Madam,

I would like to invite you to become my co-author for the following
paper. I need someone with lab facilities to carry out the experiments
needed for this project.

I need an expert like you help me get positive results in the project
(details below):-

Abstract:-
--------------

The human cells are known to be very dynamic and continuously changing
with time. They also adapt and change due to the evolution and other
external/internal factors.

Each cell has the capability to resist external pathogens and other
objects that may be unfavorable for it. Thus, there are mechanisms in
place for doing this:-
Cells have numerous defenses against invading pathogens, including
production of interferon, presentation on MHCI/II, and degradation of
incoming RNAs by dsRNA-dependent protein kinase R (PKR).

I am working on a formula that can predict the resistance of a cell
toward such pathogens in future (e.g. 45 days ,365 days , etc).
Suppose a cell has 70% capacity to resist a pathogen right now. What
will be the capacity of the cell to resist this pathogen after 365
days.

There are 2 things here to note. Firstly, we need to find the capacity
of the cell to resist at this point (Some experts might call this
immunity, but I am being specific to a specific disease i.e.
Cancer/HIV Aids here). We cannot consider this formula to be measuring
just the amount of interferon ,p53 or other cancer related parameters
as they alone do not lead to cancer.

Secondly, we need to find the future capability of this cell to resist
such pathogens. There are several parameters in play here.

Also, we need to understand these things:-
a) The cells are continuously changing due to evolution and have a
life till apoptosis.
b) The pathogens or external agents are also dynamic and evolving
with time .For diseases TB and Swine flu, the virus is changing its
shape every 6 months
c) The pathogens may combine and form a very strong hybrid strand of
pathogens, against which our cells may be weak. For e.g.) It is
elementary that the current swine flu will form a deadly strand with
HIV AIDS virus and become very deadly.
d) Both these complex systems are evolving with time and with the
life cycle and reproduction capabilities, we need to find the exact
capabilities of each of the above conditions.

What I want in a formula is the capacity (consider the unit for this
measure as immunity units/day) which will allow me to make a
probabilistically accurate measure of the resistance of the cell
against an invading pathogen in future.

For now, we consider known pathogens, but I intend to extend this to
unknown strands as well.

References:-
----------------------
Lodish et al, Introduction to Cell & Molecular Biology

Takaoka A, Hayakawa S, Yanai H, et al. (2003). "Integration of
interferon-alpha/beta signalling to p53 responses in tumour
suppression and antiviral defence". Nature 424 (6948): 516–23.
doi:10.1038/nature01850. PMID 12872134.
http://www.nature.com/nature/journal/v424/n6948/pdf/nature01850.pdf.

http://www.cehs.siu.edu/fix/medmicro/mhc.htm

Weiss RA. How does HIV cause AIDS? Science. 1993 May
8;260(5112):1273-9.URL: http://www.ncbi.nlm.nih.gov/pubmed/8493571

D J Alexander. Ecological aspects of influenza A viruses in animals
and their relationship to human influenza: a review. J R Soc Med. 1982
October; 75(10): 799–811.Online:
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1438138&blobtype=pdf
----------------------

May I request your feedback on the above topic. I have developed the
initial version of this formula which I expect to present in 1-2
months. Still, think it will be best to take the opinion of experts
like you on this topic.

I look forward to hearing from you soon. I understand that you are
very busy and I thank you for reading this message.

Please let me know if you need anything else from my side. I eagerly
await your reply

Thanks & Regards,
Jitesh Dundas

jitesh@...
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Re: Looking for Cell with unique Composition

2009-11-05T16:27:00Z

In article <hcvo89$stc$1@...>,
amdx <nojunk@...> wrote:
>Just so I'm clear, that is a single cell and the cilia are part of that
>cell.
>

Now, now, don't presume on my good nature.

--bks

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Re: Looking for Cell with unique Composition

2009-11-05T15:48:30Z

"Bradley K. Sherman" <bks@...> wrote in message
news:hcvggu$s8i$1@......

> In article <hcvfdo$huh$1@...>,
> amdx <nojunk@...> wrote:
>>High School project, looking for something different.
>>Do you know of any cells that I can research to make
>>my project different than the usual.
>>Need to make a 3D model.
>> The Mav
>>
>>
>
> Surprisingly, each cell that exists, and has ever existed, has
> unique composition.
>
> Check out these cells:
> <http://www.microscope-microscope.org/applications/pond-critters/protozoans/ciliphora/euplotes.htm>
>
> --bks
>

Thanks Bradley,
That's neat, looks so much like an insect walking on that bubble.
Just so I'm clear, that is a single cell and the cilia are part of that
cell.
Many more on the site that I'll check out too.
Thanks,
The Mav

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Re: Looking for Cell with unique Composition

2009-11-05T13:36:30Z

In article <hcvfdo$huh$1@...>,
amdx <nojunk@...> wrote:
>High School project, looking for something different.
>Do you know of any cells that I can research to make
>my project different than the usual.
>Need to make a 3D model.
> The Mav
>
>

Surprisingly, each cell that exists, and has ever existed, has
unique composition.

Check out these cells:
<http://www.microscope-microscope.org/applications/pond-critters/protozoans/ciliphora/euplotes.htm>

--bks

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Looking for Cell with unique Composition

2009-11-05T13:17:50Z

High School project, looking for something different.
Do you know of any cells that I can research to make
my project different than the usual.
Need to make a 3D model.
The Mav

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Workshop on Microarray data analysis (30.10.09 - 1.11.09)

2009-10-23T00:28:14Z

Dear friends,

We are going to conduct the workshop on microarray data analysis.

For more details visit http://www.jagsel.com/biosolutions/

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STEM CELL TECHNOLOGY PROJECTS & TRAINING

2009-10-21T23:47:20Z

"Biozone Research Technologies" & "Lifeline Hospitals"
jointly offers P.G.Diploma, Diploma, Training and Workshops in various
areas of
"STEM CELL TECHNOLOGY"
Courses Offered
Certificate Training
We provide hands on training in stem cell technology where the
Cellular characterization will be done in LIRM and Molecular
characterization will be done in BRT.
• Stem Cell Technology (1 Week)
Workshops
• Stem Cell Technology - 2 Days
• Immunology & Stem Cell Technology - 4 Days
• Cellular & Molecular Characterization of stem cells - 4 Days
• Flow Cytometry & FACS - 1 Day
In House Workshop at the Host Institution
• Stem Cell Technology - 2 Days
Projects
We also undertake students for final year projects in various areas of
stem cell research for a duration of 2-3 months.
P.G.Diploma/Diploma in Stem Cell Technology
• Advanced P.G.Diploma (Regular) 4 Months with 1 Month Project
• Advanced P.G.Diploma (Weekend) 6 Months with 1 Month Project
• Advanced Diploma (Regular) 1 Month with Optional Mini Project
• Advanced Diploma (Weekend) 2 Months with Optional Mini Project
Course Highlights
• Laboratory sessions being preceded by an expert lecture
• Experienced faculty from Lifeline Hospitals & Biozone
• Well designed Course Materials/Books/CD's
• Industrial Exposure
Eligibility
B.Tech Biotechnology, M.Sc Biotechnology, M.Sc Biochemistry, M.Sc
Microbiology, M.Sc Bioinformatics, M.Sc Life Sciences, MBBS, BVSC,
BDS, or any other equivalent degree in Life Sciences.
Registration
Registrations are open for all the courses and workshops throughout
the year.

FOR MORE INFORMATION ABOUT PROJECTS DOMAINS VISIT www.biozone.co.in
CONTACT DETAILS:
Address : Biozone Research Technologies, New No.1B, Old No. 108/72,
Kalaimagal Nagar, I Main Road, Ekkaduthangal, Chennai - 600 032.
Mobile : 9962822238
Landline/Fax : 044-22250750
Website : www.biozone.co.in
Email : info@..., biozone.info@...

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PLANT TISSUE CULTURE PROJECTS & TRAINING @ BIOZONE

2009-10-21T23:45:16Z

BIOZONE RESEARCH TECHNOLOGIES
OFFERS
PROJECTS IN PLANT TISSUE CULTURE & BIOTECHNOLOGY
Biozone, a bioscience research entity launches its Plant Tissue
Culture division to create an in-depth knowledge among the student and
research community about the importance of plant biotechnology. This
division aims at providing state of the art facility to regenerate
medicinal plants, economically important crops, ornamental & aromatic
plants and saving the endangered species through tissue culture
techniques.
TRAINING MODULES:
• Plant Tissue Culture
• Analysis of Herbal Plants
• Plant Molecular Biology Techniques
PROJECT DOMAINS
• Direct and Indirect Organogenesis
• Invitro plant regeneration from endangered medicinal and aromatic
plants
• Secondary metabolite production through callus and cell suspension
plants
• Phytochemical Analysis of secondary metabolite
• Protoplast culture technology
• Antioxidant and Activity
• Antimicrobial activity
• Precursor and elicitor study
• Immobilization techniques
• Genetic Fedility test
FOR MORE INFORMATION ABOUT PROJECTS DOMAINS VISIT www.biozone.co.in
CONTACT DETAILS:
Address : Biozone Research Technologies, New No.1B, Old No. 108/72,
Kalaimagal Nagar, I Main Road, Ekkaduthangal, Chennai - 600 032.
Mobile : 9962822238
Landline/Fax : 044-22250750
Website : www.biozone.co.in
Email : info@..., biozone.info@...
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BIOTECHNOLOGY AND BIOINFORMATICS PROJECTS @ BIOZONE

2009-10-21T23:43:53Z

BIOZONE RESEARCH TECHNOLOGIES
OFFERS
PROJECTS IN BIOTECHNOLOGY AND BIOINFORMATICS
Biozone Research Technologies, a bioscience training and research
entity designs and delivers innovative research and training solutions
to pharmaceutical and life sciences industry. Our state-of-the-art
infrastructure and technical competencies enable us to offer high-end
research projects in Biotechnology, Bioinformatics and various fields
of life science.
PROJECT DOMAINS OFFERED
BIOTECHNOLOGY
• Gene cloning and sequencing
• DNA fingerprinting & molecular marker development
• Analysis of chloroplast & mitochondrial genomes for genotyping
• Ribotyping analysis
• Immunology
• Strain improvement
• Bioremediation
• Herbal medicine
• Molecular diagnostics
• Industrial biotechnology applications
• Proteomics
• Medical biochemistry
• Novel compound identifications of medicinal value
• Protein profiling and Isoenzyme analysis
• Biotransformation
• Epidemiological studies of infectious clinical isolates
• Molecular analysis of drug resistance
BIOINFORMATICS
• Genome Analysis
• Protein Structure Analysis and Fold recognition
• Functional Annotation of Hypothetical Proteins
• Protein Modeling and Validation
• Protein Structure Comparison based on Mutations
• Molecular Modeling and Docking
• Insilico Expression Analysis to identify potential drug targets
• Identification of Drug targets by Comparative Approaches
• Subtractive methods for Drug target prediction and Validation
• Quantitative Structure Activity Relationship Analysis and ADME
• Development of Biological Databases using Bioprogramming
• Algorithm Development
FOR MORE INFORMATION ABOUT PROJECTS DOMAINS VISIT www.biozone.co.in
Contact Details:
Address : Biozone Research Technologies, New No.1B, Old No. 108/72,
Kalaimagal Nagar, I Main Road, Ekkaduthangal, Chennai - 600 032.
Mobile : 9962822238
Landline/Fax : 044-22250750
Website : www.biozone.co.in
Email : info@..., biozone.info@...

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sent from wrong email address- UV sterilization???

2009-09-23T15:04:33Z

Hi Jens,

I saw your post about the CL-1000 crosslinker. I have one myself in the lab
and am trying to figure out how long my nylon membrane needs to sit inside
the crosslinker for the DNA to hybridize. You said the setting is 120,000
microjoules per cm2 but for how long?

Thanks,
Valery
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Reactome Pathway Database User Survey

2009-09-22T14:04:43Z

Reactome is committed to providing access to high-quality pathway information and helpful data analysis tools. With this in mind, we are actively soliciting comments from the research community in order to assess community needs. We are interested to hear about your experience with Reactome, and would like to know a bit about your background and research interests so that we can continue to improve the Reactome site and tools.

You can access the survey at: http://tinyurl.com/l48zzq

Thank you for taking part.

Robin Haw
Manager of Reactome Outreach
Outreach [at] reactome.org
http://www.reactome.org
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Distance courses in bioinformatics and systems biology

2009-09-21T23:47:15Z

At the University of Manchester, UK, we have over 10 years experience in
providing distance courses, at Masters level, to participants from a
range of backgrounds, including Biology, Healthcare, Medicine and
Computer Science. These courses are designed to enable biologists and
computer scientists to update their skills while remaining in the
workplace. Our distance courses are also very suitable for home study,
helping you to keep in touch with a research environment while taking a
career break. Participants continuing to Masters level will complete a
research project.

The courses beginning on 5 October 2009 include:

- Introduction to Bioinformatics
(http://octette.cs.man.ac.uk/bioinformatics/modules/bs61061.html)
- Bioinformatics for Systems Biology
(http://octette.cs.man.ac.uk/bioinformatics/modules/BIOL61820.html)

You will find further information, including fees and a link to the
online application form, here:
http://octette.cs.man.ac.uk/bioinformatics/index.html If you have any
questions, or need advice on the course options, please contact
Heather.Vincent@...

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WS1

2009-09-21T11:01:42Z

Hi Marie,

Do you still have the problem with these cells. Did you buy it from ATCC. Is it CRL-2029?

Thanks.

Zareen

[Cell-biology] WS1 cells
Kirchner, Marieluise Marieluise_Kirchner at hms.harvard.edu
Tue May 16 18:03:16 EST 2006
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I received recently WS1 human fibroblast cells and I am trying to culture them. But it seems that they are not growing very well (close to "not at all") in my hands. Does maybe somebody has some experiences with this cell line and can give me some suggestions how to culture them? That would be very helpful. Thanks Marie -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/cellbiol/attachments/20060516/cab17cda/attachment.html

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RE: Request For Help

2009-09-14T14:26:25Z

The paper from bell Labs IS very interesting. But from your answer (from the organelles you are interested in), it is clear that you intend to work on eukaryotic cells, but it is still not clear what kind of cells you have in mind, since each eukaryotic cell is a highly specialized "piece of equipment", nor is it clear to what extend you want to take your experiments forward and the expected observation you intend to make. Do you have a hypothesis which you intend to test?? That would help.
As you might know, the immune system is replete with similar memory responses, especially related to plasma cells and long term immunity against diseases. I would suggest researching papers on plasma cell longevity, long term immunity versus short lived immunity and memory T-cells in literature. It has been an enigma how plasma cells in the bone marrow recognize and maintain immunity by producing antibodies against certain pathogenic antigens like that of small pox virus, when they don't do the same for other antigens like the cold virus. A clue might be that the small pox virus is dangerous while the cold virus is not. You should proceed in that direction with your experiments.. Two variables in your experiments should be TIME (intervals between exposures) and INTENSITY (concentration and number) of the stimulus. An experiment to study such memory responses in cells should consist of repeated exposure of the cell you have in mind to a stressful stimulus(protein, heat, nutrient deprivation or some other factor towards which the particular cell type or types you are interested in are specialized to respond to), varying the time periods of exposure and the dosage of the stimulant. Studying chromosomal translocations.
Please correct me, if I am barking up the wrong tree :-)). Again a hypothetical model which you aim to test, with aims would be useful for people to help you with experiments.
Bye
Best ofluck
Jay

dddddddddddddddddddddddddddddddddddddddddddddddddddd
Jayakumar R. Nair, Ph.D.,
HRI Scientist,
Dept. of Immunology,
Roswell Park Cancer Institute,
Elm and Carlton St.
Buffalo, NY 14263
Tel: 716-845-8231
email: r.jayakumar@...
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa

"Nil magnum nisi bonum"

-----Original Message-----
From: jitesh dundas [mailto:jbdundas@...]
Sent: Monday, September 14, 2009 2:54 PM
To: Jayakumar, R
Subject: Re: [Cell-biology] Request For Help

Dear Jay,

Thank you for your reply.

Your question is very vague.
Sorry I don't agree with you. I was trying to keep it brief. In fact, you are the first expert who didn't get my question. I guess every person thinks differently.

you need to be more specific.
Yes, I am provided I get a positive response from an expert like you.
Morever, I am cautious of not providing my research paper details to anyone without authentication.

What is "thinking capabilities" of cell organelles and what kind of organisms are you planning to study?
WELL, as humans have brain for thinking & control of major operations.I want to verify/investigate if cells can do the same. I maybe looking at the cell/brain analogy besides studying cell behavior.To what level do cells reflect brain-like behavior..

Few papers (one froma bell laba@pubmed titled -can cells think?)

And what are the "tests" or experiments you have in mind? Please elaborate.

First I just want to study cellular behavior in different conditions like extreme temperature, pathogen infiltration,etc..

Nucleus is my primary organelle of interest besides mitochondria, edoplasmic reticulum,etc.

I am looking at all possible ways to find if cells can reproduce creative thinking capabilities.

I hope this helps. Please suggest computational tools and wet experiments suitable for this hypothesis.

Regards,
Jitesh Dundas

On 9/14/09, Jayakumar, R <R.Jayakumar@...> wrote:

> Dear Jitesh
> Your question is very vague. you need to be more specific. What
> is "thinking capabilities" of cell organelles and what kind of
> organisms are you planning to study? And what are the "tests" or
> experiments you have in mind? Please elaborate.
>
> Jay
>
>
> ________________________________
> From: cellbiol-bounces@...
> [cellbiol-bounces@...] On Behalf Of jitesh dundas
> [jbdundas@...]
> Sent: Monday, September 14, 2009 5:43 AM
> To: cellbiol@...
> Subject: [Cell-biology] Request For Help
>
> Dear Sir/Madam,
>
> I am studying the cell organelles in uni-cellular & multi-cellular
> organisms for their thinking capabilities.I have some tests to perform
> & I would appreciate if some expert in (cell-biology) could help me in
> providing experimental data for these tests.
>
> The tests are very basic & may have been already done. I open to some
> suggestions on any new experiments for my research..
>
> Please let me know if you need anything else from my side.
>
> Regards,
> Jitesh Dundas
> http://jiteshbdundas.blogspot.com<http://jiteshbdundas.blogspot.com/>
> _______________________________________________
> Cellbiol mailing list
> Cellbiol@...
> http://www.bio.net/biomail/listinfo/cellbiol
>
>
> This email message may contain legally privileged and/or confidential
> information. If you are not the intended recipient(s), or the
> employee or agent responsible for the delivery of this message to the
> intended recipient(s), you are hereby notified that any disclosure,
> copying, distribution, or use of this email message is prohibited. If
> you have received this message in error, please notify the sender
> immediately by e-mail and delete this email message from your computer. Thank you.
>

This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.
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RE: Request For Help

2009-09-14T10:42:24Z

Dear Jitesh
Your question is very vague. you need to be more specific. What is "thinking capabilities" of cell organelles and what kind of organisms are you planning to study? And what are the "tests" or experiments you have in mind? Please elaborate.

Jay

________________________________
From: cellbiol-bounces@... [cellbiol-bounces@...] On Behalf Of jitesh dundas [jbdundas@...]
Sent: Monday, September 14, 2009 5:43 AM
To: cellbiol@...
Subject: [Cell-biology] Request For Help

Dear Sir/Madam,

I am studying the cell organelles in uni-cellular & multi-cellular
organisms for their thinking capabilities.I have some tests to perform
& I would appreciate if some expert in (cell-biology) could help me in
providing experimental data for these tests.

The tests are very basic & may have been already done. I open to some
suggestions on any new experiments for my research..

Please let me know if you need anything else from my side.

Regards,
Jitesh Dundas
http://jiteshbdundas.blogspot.com<http://jiteshbdundas.blogspot.com/>
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This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

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Request For Help

2009-09-14T02:43:27Z

Dear Sir/Madam,

I am studying the cell organelles in uni-cellular & multi-cellular
organisms for their thinking capabilities.I have some tests to perform
& I would appreciate if some expert in (cell-biology) could help me in
providing experimental data for these tests.

The tests are very basic & may have been already done. I open to some
suggestions on any new experiments for my research..

Please let me know if you need anything else from my side.

Regards,
Jitesh Dundas
http://jiteshbdundas.blogspot.com
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Protein Concentration Question

2009-08-27T21:52:14Z

Hey Erik,
I saw your question posted on bio.net and was wondering if you ever figured out the answer. I had a similiar question: What is the concentration of a protein in a cell in molarity? I know that proteins account for 15% of a cell's overall concentration and that the molar mass of a protein is 50,000 g/mol. If you could help me figure out the answer, that would be great.

Thank you!
Mina

Hi netters. Can anyone be of help by telling me where/how I might be able
to find/calculate a "typical" concentration (mg/ml or something of the
like) of a housekeeping protein like tubulin in a "typical" mammalian
cell. I need a ball park figure. As far as I know, about 16-20% of a
cell's weight is protein (but what's the typical weight of a "typical"
mammalian cell?), volume is readily estimated from cell diameter, and a
highly expressed protein could be about 1% of the total protein in a cell
(so for, say tubulin, about 0.1-0.2% of the cell is protein by weight
(?)). Any suggestions, info???? Please email.

Cheers,

erik

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Trypsin

2009-08-21T06:21:22Z

Hi,
I have a problem with detaching my cells: I use 1x Trypsin (0.5 mg/ml) with EDTA to detach HeLa cells. But after incubation for some minutes at 37 °C there are huge cell aggregates that do not seperate, even when I try to resuspend them by pipetting up and down. I tried it hard, but no chance. Moreover, when I want to pellet the cells, the aggregates are not soluble and because of that, I'm not able to centrifuge them down. Besides that, the suspension of trypsin and cells shows a strange behaviour on the plate: when I try to take out the suspension from the plate with the pipet, a big amount of suspension tries to redistribute on the plate even if I hold the plate slantwise. Perhaps some strange effects of surface tension.

I hope somebody can help me. Perhaps its a simple mistake I am doing, because I am relatively new in that cell culture things. My colleagues say a little bit of that 'redistribution effect' is normal.

Cheers,

Daniel
--
GRATIS für alle GMX-Mitglieder: Die maxdome Movie-FLAT!
Jetzt freischalten unter http://portal.gmx.net/de/go/maxdome01

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(no subject)

2009-08-20T22:39:28Z

Can anbody please clarify the following doubts I have?

Phytases are enzymes usefull in hydrolzing phytate ie.the organic source of phosphorus. Acid phosphatases are enzymes which solubilize inorganic phosphorus.

In the literatue available it is mentioned that phytases are a gruop of phosphatases and some people say phosphatases are a group of phytases

Which is correct? and I would like to know the classification of both phytases and phosphatases. could anbody in this field help me out

Aftab from Bangalore India
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Where to buy pentosidine?

2009-08-20T07:41:47Z

Hy Mr.Dunn,

I saw in Google your message from 1996 about "Where to buy pentosidine?".
I have the same question. Did you got an answer and was there any company who delivered this compound?

Kind regards,

Ing.Jan Visser
University Centre for Pharmacy
Department of Pharmacokinetics, Toxicology & Targeting
Ant.Deusinglaan 1
9713 AV Groningen
The Netherlands
tel. 31-50-3633280
fax 31-50-3633247
e-mail: Jan.Visser@...

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Tips for Retrovial Transfection

2009-08-18T11:37:43Z

I'm not getting any positive cells in my titers. I'm using 293T,
lipofectamine, and a Nature protocol with the DNA described therin (a GFP
reporter).
(Paper: Retrovirus-mediated single-cell gene knockdout technique in adult
newborn neurons in vivo, Tashiro and Gage).

Pretty standard protocol as far as I know. I didn't purify the virus by
ultracentrifugation and just did titers with filtered viral sups instead. No
positives by FACS.

Where are the most common pitfalls? As far as I can tell, my cells are
good, DNA is good, Lipofectamine is expensive, and the protocol was followed
with a small change.

Thank you for any help!
--
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Practical Course in Biomolecular Modelling

2009-08-14T07:33:51Z

Dear colleagues,

please be informed that online applications are accepted for the
following course:

8TH NCCR PRACTICAL COURSE IN BIOMOLECULAR MODELLING

January 10 - 15, 2010
Kandersteg, Switzerland
http://www.structuralbiology.uzh.ch/course2010.asp

Course topics include
Simulation techniques, force-field development, conformational search,
computation of free energy and entropy, treatment of electrostatic
forces, simulation of folding, comparison of simulation with experiment

This course is primarily directed to PhD students and postdocs from
experimental structural biology groups wishing to learn more on
biomolecular modelling. The course format will include morning lectures
and late-afternoon/early evening tutorials, and provide ample
opportunities for discussions with experts and fellow participants.
Participants will be invited to bring own problems for tutorials and/or
discussion. The course will be organized as a winter retreat in the
Swiss Alps offering a stimulating learning atmosphere with the
afternoons available for informal participation in discussions, reading
and self-study or recreational activities in the area.

Interested candidates are encouraged to apply online on
http://www.structuralbiology.uzh.ch/course2010_application.asp.
Application deadline will be October 16, 2010. We will be able to accept
20 participants to this course.

Best regards,
Patrick Sticher

--
_________________________________
Visit the NCCR on the Internet
www.structuralbiology.uzh.ch

Dr. Patrick Sticher Moser
NCCR Scientific Officer
Institute of Biochemistry
University of Zürich
Winterthurerstrasse 190
CH - 8057 Zürich

Phone +41 / (0)44 / 635 54 84
Fax +41 / (0)44 / 635 59 08
Mail sticher@...

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need your help

2009-08-13T04:01:43Z

Hi,

This is Dr Bhawana Gupta from India. I want to do transfection in floating
cells either in U937 or in HL60. I procured Fugene hd from Roche. Do you
have any experience of tranfecting floating cells and making stable cell
lines. I couldn't get any specific protocol regarding selection of these
tranfected floating cells and how to separate live cells from dead once,
because we can not spin such a lower no of cells during selection. If you
have related knowledge, please share with me. I am in acute need.

Thanks & Best Regards

Dr. Bhawana Gupta
M.V.Sc.

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NCCR Symposium on New Trends in Structural Biology / NCCR Practical Course on Biomolecular Modelling

2009-07-17T06:51:05Z

Dear colleagues,

we have two announcements to make:
_____________________________________________________________

7th International NCCR Symposium on New Trends in Structural Biology
7 + 8 September 2009, ETH Zürich, Lecture Hall HG E7, Zürich, Switzerland
www.structuralbiology.uzh.ch/symposium2009

The lecture program is available online at:
http://www.structuralbiology.uzh.ch/symposium2009/program.asp

Plenary lecturers:
Frédéric Allain, Pietro de Camilli, Raimund Dutzler, Arthur L. Horwich,
Brian Kobilka, Harry Noller,
Anna Marie Pyle, Ben Schuler, David Wemmer, Masasuke Yoshida

Online registration to this event is still possible through the
symposium homepage or directly at:
http://www.structuralbiology.uzh.ch/symposium2009/registration09.asp
_____________________________________________________________

8th NCCR Practical Course and 2nd Winter School
Biomolecular Modelling

The 8th NCCR Practical Course will cover key topics in the area of
computational structural biology. The course format includes morning
lectures by experts in the field that alternate with later afternoon to
evening tutorials and discussions. The course will be organized as a
winter retreat in the Swiss alps offering participants the opportunity
to learn in a stimulating atmosphere, enjoy winter activities in the
swiss alps during course breaks, and network and exchange ideas on an
informal basis with fellow participants, tutors, and lecturers.

More information at http://www.structuralbiology.uzh.ch/course2010.asp
Online Application will be possible as of July 31, 2009
_____________________________________________________________

Please do not hesitate to contact me anytime if you need further
information (sticher@...).

With best regards,
Patrick Sticher

The NCCR Structural Biology is a research initiative of the Swiss
Science Foundation. Its research encompasses the fields of recombinant
protein technologies, macromolecular structure determination and
computational biomolecular sciences with a special focus on membrane
proteins and supramolecular assemblies/interactions. 13 research groups
from Swiss Universities and Research Institutions participate in this
network. www.structuralbiology.uzh.ch/

--
_________________________________
Visit the NCCR on the Internet
www.structuralbiology.uzh.ch

Dr. Patrick Sticher Moser
NCCR Scientific Officer
Institute of Biochemistry
University of Zürich
Winterthurerstrasse 190
CH - 8057 Zürich

Phone +41 / (0)44 / 635 54 84
Fax +41 / (0)44 / 635 59 08
Mail sticher@...

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SV-40 control block/slides?

2009-07-14T11:20:25Z

I am trying to locate known positive tissue to the SV-40 antibody
either in a formalin fixed paraffin embedded form or on cut slides.
Any help would be much appreciated. Thanks for your help!

Jeremy Johnston
jjohnston@...
206-374-9000

This e-mail message, including any attachments, is for the sole use of the
intended recipients and may contain privileged information. Any unauthorized
review, use, disclosure or distribution is prohibited. If you are not the intended
recipient, please contact the sender by e-mail and destroy all copies of the
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.
at (206) 374-9000.

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National Conference on Stem cell Research, Computational Biology and Chemistry on 8th & 9th of August at Hotel Green Park, Chennai

2009-06-17T05:40:33Z

Dear all,

BionteQ Bioscience Private Limited, a company located in Chennai,
venturing into Bioinformatics, Cheminformatics and Bio-Medical
Engineering providing software and research solutions to the life
science world. The company also provides Consultancy Services in the
area of computational biology and chemistry with its own research and
development team working on these areas.

BionteQ is organizing Technobia ’09, a National Conference on Stem
cell Research, Computational Biology and Chemistry on 8th and 9th of
August at Hotel Green Park.

Technobia’09 aims to bring together researchers, scientists, students,
engineers, and academic and R&D scholars to exchange and share their
experiences, new ideas, and research results about all aspects of
Computational Biology & Chemistry and also Stem cell research, and
discuss the practical challenges encountered and the solutions
adopted. The event addresses the development and application of
computational methods for biological problems from the perspectives of
both research and industry. Eminent well known speakers come together
to enlighten the audience with their research, academic and industry
experiences, also highlighting the recent research and scope in these
fields.

The Conference covers the following topics:

STEM CELL RESEARCH

1. Dr. Rama Shanker Verma, Associate Professor, Stem and Molecular
Biology lab, Department of Biotechnology, IITM, Chennai

2. Dr. Shaji R.V, Assistant Professor, Department of Haematology, CMC,
Vellore.

CHEMINFORMATICS

• Dr. Chandan Raychaudhury, Solutions Scientist, Life Science,
Accelrys, Bangalore

DRUG DESIGNING

• Mrs. Rohini Srinivasan, Senior Consultant, Lifesciences and Pharma,
HCL Technologies, Chennai

SAS & CLINICAL DATA MANAGEMENT

• Mr. Ahmed Z Kabeer, Project Manager, Laxai, Hyderabad.

STRUCTURAL BIOINFORMATICS

• Dr. Swagatha Das Gupta, Associate Professor, Department of
Chemistry, IIT Karaghpur.

SYSTEMS BIOLOGY

• Mr. Saurabh Bundela, Senior Scientist, Systems Biology India Pvt
Ltd, Pune.

MICRO ARRAY

• Dr. Kshitish Acharya, Faculty Scientist, IBAB, Bangalore.

REGISTRATION

Early Bird Offer
Upto June 30,
2009 July 1 onwards
Students INR
1850/- INR 2000/-
Academic and
R&D professionals INR
2850/- INR 3000/-
Industry INR
3850/- INR 4000/-

The registration fee is to be paid through demand draft drawn in
favor of BionteQ Bioscience Private Limited payable at Chennai.

Registration fee includes the following:
Conference kit, Certificate, Lunch and refreshments.

ACCOMODATION

Participants should arrange their own accommodation, however,
accommodation in hotels, paying guest houses may be booked on request,
against an advance booking amount sent. A special request must be made
for accommodation assistance during the registration.

The request for accomodation must reach BionteQ by July 31, 2009.
Payments should be made through a demand draft in favor of BionteQ
Bioscience Private Limited payable at Chennai.

For any further information please contact:

Coordinator (Technobia ‘09),
BionteQ Bioscience Private Limited
No.97/5, Periyar Pathai,
Choolaimedu,
Chennai-600094
Tel:+91-44-64536777
Mob: 9282348999
Email: info@...
www.bionteq.com

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anti VSVG

2009-06-08T11:23:04Z

Did somebody suggest back an useful antibody. Have the same problem myself,
want ectodomain antibody.

------------------
Brian Storrie, Ph.D.
Professor of Physiology and Biophysics
University of Arkansas for Medical Sciences
4301 West Markham Street
Little Rock, AR 72205

Telephone: (501) 526-7418
Fax: (501) 686-8167
Cell phone: (501) 690-1726

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Request for co-author/reviewer in my paper

2009-06-06T20:36:56Z

Dear Sir,

I am looking for a co-author/reviewer in the field of cell
biology/nanomedicine who can help me in improving my research paper.

Please reply if you would like to look at my paper draft and could help me out.
--
Thanks & Regards,
Jitesh Dundas

Phone:- +91-9860925706

http://jiteshbdundas.blogspot.com

"NO IDEA IS STUPID,EITHER IT IS TOO GOOD TO BE TRUE OR IT IS WAY AHEAD
OF ITS FUTURE "- GEORGE BERNARD SHAW.

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Call for Papers

2009-06-03T02:32:41Z

*Journal of Cell and Animal Biology*

www.academicjournals.org/JCAB

Paper

Dear Colleague,

*Introducing ‘Journal of Cell and Animal Biology (JCAB)’*

The Journal of Cell and Animal Biology (JCAB) publishes high-quality
solicited and unsolicited articles, in English, in all areas of cell and
animal biology. The following types of papers are considered for
publication:

· Original articles in basic and applied research.

· Critical reviews, surveys, opinions, commentaries and essays.

Our objective is to inform authors of the decision on their manuscript(s)
within four weeks of submission. Following acceptance, a paper will normally
be published in the next issue.

Instruction for authors and other details are available on our website
www.academicjournals.org/JCAB. Prospective authors should send their
manuscript(s) to jcab.acadjourn@... or jcab_academicjournals@...

*Open Access*

One key request of researchers across the world is unrestricted access to
research publications. JCAB is fully committed Open Access Initiative by
providing free access to all articles (both abstract and full PDF text) as
soon as they are published. We ask you to support this initiative by
publishing your papers in this journal.

*Publication Alert*

We will be glad to send you a publication alert showing the table of content
with link to the various abstracts and full PDF text of articles published
in each issue. Kindly send us an email if you will like to receive
publication alert.

Best regards,

*Dr. Mohammed Nahmoud,*

Editor

Journal of Cell and Animal Biology (JCAB)

E-mail: jcab.acadjourn@... <%20jcab.acadjourn@...>

http://www.academicjournals.org/JCAB
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the data of tissue distribution of apolipoprotein E mRNA

2009-06-02T17:22:30Z

Hi all,

Do you know where I can get the data or the figure of the tissue
distribution of apolipoprotein E mRNA? Or is there a such database that
contains that information? I want to get the column figure of tissue
distribution of apoE.

Thanks.

Qiuhui
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Re: TDGA..where to get it?&In-Reply-To=

2009-05-30T03:29:25Z

Hey Carole:
Hope you are doing good. I guess TGDA is probably the same as methyl
palmoxirate. Can be obtained from R.W. Johnson Pharmaceutical research
Institute.

Regards,
Srikant

On Apr 29, 8:02 pm, "Carole Sztalryd-Woodle"
<cszta...@...> wrote:

> I am also looking for it, did you get any answers?
>
> Carole Sztalryd
> Assistant Professor of Medicine
> Division of Endocrinology
> HH room 445A
> 650 West Redwwod Street
> Baltimore MD 21201
> (office) 1-410-706-4047
> (lab) 1-410-706-3505 or 3594
>
> Confidentiality Statement:
> This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message.
>
> Carole Sztalryd-Woodle1.vcf
> < 1KViewDownload

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Re: Molecular Therapy of Breast Cancer: Classicism Meets Modernity (announcing)

2009-05-20T07:12:33Z

In article <ebd8cadd-7706-4b99-becf-378e176383a6@...>,
<scimedweb@...> wrote:
>Molecular Therapy of Breast Cancer: Classicism Meets Modernity (book).
>by Marc Lacroix
> ...
>Here are described nearly 150 drugs that are currently used in routine
> ...

The Torture discussion belongs in talk.politics.misc. This group
is reserved for discussions of Science.

--bks

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Molecular Therapy of Breast Cancer: Classicism Meets Modernity (announcing)

2009-05-20T02:19:45Z

Molecular Therapy of Breast Cancer: Classicism Meets Modernity (book).

by Marc Lacroix
InTextoResearch, Baelen (Liège, Wallonia), Belgium
Nova Sciences Publishers, New York, ISBN 978-1-60741-593-0

https://www.novapublishers.com/catalog/product_info.php?cPath=23_29&products_id=10042&osCsid=8fed1efc6158a0ba4004bec047b66ff0

Breast cancer is the most frequently diagnosed type of cancer and a
second leading cause of cancer death in women after lung cancer.
Despite their proven efficacy, classical therapies are, however,
unable to cure metastatic breast cancer and are often associated with
significant toxicity and side-effects, due to a wide spectrum of
action. During the last years, our increasing knowledge of the
molecular pathways underlying cancer development has led to the
introduction of new drugs, of which most are directed towards very
specific targets. Rather than to be used as single agents, these
“modern” compounds could ultimately be combined with classical
molecules.
Here are described nearly 150 drugs that are currently used in routine
therapy or are in clinical trials in breast cancer patients. From the
classical tamoxifen, fluorouracil, cyclophosphamide, doxorubicin,
epirubin, docetaxel, paclitaxel…, to the more recently introduced
ixabepilone, lapatinib, vorinostat, everolimus, bevacizumab…, they
also include capecitabine, gemcitabine, trastuzumab, bevacizumab,
fulvestrant, aromatase inhibitors, cancer vaccines, inhibitors of
tumor-induced osteolysis, insulin-like growth factor-I receptor
inhibitors, poly(ADP-ribose) polymerase (PARP)-1 inhibitors, and many
others.
This book offers an insight into current developments of breast cancer
therapy, when classicism meets modernity.

Table of Contents:
Chapter 1. Introduction

Chapter 2. Selective estrogen receptor modulators (SERMs) and down-
regulators (SERDs)

Chapter 3. Aromatase inhibitors

Chapter 4. Agents inducing ovarian suppression

Chapter 5. Antimetabolites

Chapter 6. Alkylating agents

Chapter 7. Anthracyclines

Chapter 8. Microtubule-binding agents

Chapter 9. Topoisomerase inhibitors

Chapter 10. HER family inhibitors

Chapter 11. Angiogenesis inhibitors

Chapter 12. Insulin-like growth factor-I receptor inhibitors

Chapter 13. RAS-RAF-MEK-ERK pathway inhibitors

Chapter 14. Ubiquitin-proteasome system inhibitors

Chapter 15. Histone deacetylases inhibitors

Chapter 16. Mitotic inhibitors

Chapter 17. Inhibitors of heat-shock proteins 90 and 27

Chapter 18. PI3K/AKT/mTOR pathway inhibitors

Chapter 19. Cyclooxygenase-2 inhibitors

Chapter 20. Poly(ADP-ribose) polymerase (PARP)-1 inhibitors

Chapter 21. Tumor-induced osteolysis inhibitors

Chapter 22. Vaccines and immunomodulators

Chapter 23. Varia

Index

List of drugs/compounds discussed in the book:

ABT-888, ABT-869, Ad-sig-hMUC-1/ecdCD40L vaccine, AE37 peptide/GM-CSF
vaccine, Aflibercept, AFP464, AG014699, Alendronate, Aldesleukin,
Allogeneic GM-CSF-secreting breast cancer vaccine, ALT-801, AMG 386,
AMG 479, Anastrozole, Apricoxib, ARRY-334543, AS1402, ATN-224,
Autologous dendritic cell-adenovirus p53 vaccine, AV-951, AVE1642,
AVX701, AZD0530, AZD6244, Bavituximab, Belinostat, Bevacizumab,
BGT226, BIBW 2992, BMS-754807, Bortezomib, Bosutinib, BSI-201,
Busulfan, BZL101, Capecitabine, Carboplatin, CDX-1307, Celecoxib,
Cetuximab, CHP-HER2, CHP-NY-ESO-1, Cisplatin, Cixitumumab, Clodronate,
CNF2024, CR011-vcMMAE, Cyclophosphamide, Dasatinib, Deforolimus,
Denileukin diftitox, Denosumab, Docetaxel, Doxorubicin, Entinostat,
Enzastaurin, Epirubicin, Eribulin , Erlotinib, Ertumaxomab, Etoposide,
Everolimus, Exemestane, Falimarev, Figitumumab, Fluorouracil,
Fulvestrant, Gefitinib, Gemcitabine, Goserelin, GP2 peptide/GM-CSF
vaccine, GRN163L, GSK1059615, HER2/neu (extracellular domain) peptide
vaccine, hTERT/Survivin Multi-Peptide Vaccine, Ibandronate,
Ifosfamide, Imatinib, IMP321, Inalimarev, Interleukin-12, Irinotecan,
Ispinesib, Ixabepilone, Lapatinib, Larotaxel, Letrozole, Leuprolide,
Lonafarnib, Lonaprisan, Mammaglobin-A DNA vaccine, Melphalan,
Methotrexate, MK0646, MK-0752, MLN8237, Modified vaccinia Ankara
(Bavarian Nordic)-HER2 vaccine, Neratinib, NVP-AUY922, NVP-BEZ235,
Odanacatib, OGX-427, Olaparib, OSI-906, Ovarian Cancer Peptide Mix
Immunotherapeutic Vaccine, Oxaliplatin, Paclitaxel, Panobinostat,
Patupilone, Pazopanib, PD-0332991, Pemetrexed, Pertuzumab,Picoplatin,
pNGVL3-hICD vaccine, PTC299, Ramucirumab, Recombinant fowlpox-CEA(6D)/
TRICOM vaccine, Recombinant vaccinia-CEA(6D)-TRICOM vaccine,
Retaspimycin, Rexin-G, Risedronate, Sagopilone, SCH 727965, Sialyl
Lewisª-keyhole limpet hemocyanin (KLH) conjugate vaccine, Sirolimus,
Sorafenib, Sunitinib, Tamoxifen, Telomerase: 540-548 peptide vaccine,
Temozolomide, Temsirolimus, Tesmilifene, Thiotepa, Tipifarnib,
Toremifene, Trabectedin, Trastuzumab, Trastuzumab-MCC-DM1,
Triptorelin, TTK peptide (mixed with adjuvant Montanide ISA-51),
Valproic Acid, Vandetanib, Vinflunine, Vinorelbine, Vorinostat,
WX-671, Zoledronic Acid
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Bioinformatics Summer training & Projects

2009-05-17T21:33:30Z

Greetings from iPower Solutions India Ltd, Chennai

iPower Solutions India Ltd is a premier training institute in Chennai
offering a Research and Industry oriented training in
Bioinformatics. We are specializing in Course Training, Certificate
Course, PGDBI, Project Guidance, and Summer Training in diverse
application areas.

Bioinformatics Course Training:
Duration: 2 Weeks

Introduction to Bioinformatics
Bioinformatics tools and databases
Proteomic tools and Databases
Genomic tools and databases
Introduction to genomics
Introduction to Proteomics
Introduction to Drug designing &Software’s

Inplant /Summer Projects Domains:
Duration: 1 Month

Comparative Genomics
DNA Sequence analysis
Drug Designing
Docking
Gene prediction
Herbal Informatics
Microbial Genomics
Protein Sequence analysis
Phylogenetic analysis.
System biology

Further information contact to us:
iPower Solutions India Ltd,
Bioinformatics Coordinator,
Adambakkam, Chennai-600 088
Website: www.ipbioinformatics.com
E-mail: project@...
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