Nabble - Bio.net - Cytonet

New Flow Cytometry Software for ACS 1.0 standard

2009-10-11T11:23:01Z

Hi,

I have developed a new flow cytometry program that can read a partial
list of the ACS 1.0 standard. The program can process parByte, int32,
and parShort list mode files. I have made it open and posted it on:

https://sourceforge.net/projects/kflowcacs/

Let me know what you think.

Diana
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Thrombin

2006-06-13T16:40:33Z

Thrombin

Hi
I'm a Cytotech, working in Saskatchewan/ Canada and am looking for information to make cell blocks using plasma/thrombin method. Which company supplies thrombin? Does it need to to be refrigerated?

June

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mitotic spindle fibers

2006-04-07T15:28:11Z

Hi,
I'm a biologist who works in a genetics laboratory.
I'd like know if exists a method for stain mitotic spindle fibers
without use rabbits antibody
someone can help me? Thanks

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Get your Free Cell here Now!

2006-04-02T13:42:11Z

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-02T12:16:27Z

In article <1138906329.924496.122480@...>, tressure@... wrote:
>> >What's that definition of insanity again? Doing the same thing over
>> >and over, and expecting different results?
>> Call your doctor. You can pay him or her to give you an answer.
>
>If that reply was directed at me, should I point out that the question
>was rhetorical?
>

Sorry. The suggestion was for the OP.
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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-02T10:52:09Z

> >What's that definition of insanity again? Doing the same thing over
> >and over, and expecting different results?
> Call your doctor. You can pay him or her to give you an answer.

If that reply was directed at me, should I point out that the question
was rhetorical?

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-02T10:40:15Z

In article <1138904667.832785.47380@...>, tressure@... wrote:

>> WTF?! I posted it to biology, medical, and chemistry newsgroups! They
>> should know and care, because my question involves biological, medical,
>> and chemical entities!
>
>Sorry-my mistake. I forgot that you would be a much better judge than
>I of what I am interested in. The fact that you haven't got the answer
>you wanted after umpteen thousand repititions of the question must be
>an experimental error, and not an indication that nobody cares.
>
>So go ahead- ask the question again, and again, until you get the
>answer you want.
>
>What's that definition of insanity again? Doing the same thing over
>and over, and expecting different results?
>

Call your doctor. You can pay him or her to give you an answer.
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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-02T10:24:27Z

> WTF?! I posted it to biology, medical, and chemistry newsgroups! They
> should know and care, because my question involves biological, medical,
> and chemical entities!

Sorry-my mistake. I forgot that you would be a much better judge than
I of what I am interested in. The fact that you haven't got the answer
you wanted after umpteen thousand repititions of the question must be
an experimental error, and not an indication that nobody cares.

So go ahead- ask the question again, and again, until you get the
answer you want.

What's that definition of insanity again? Doing the same thing over
and over, and expecting different results?

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T17:22:18Z

Spaceman wrote:

> "Radium" <glucegen1@...> wrote in message
> news:1138840980.823758.232290@......
> | tressure@... wrote:
> | WTF?! I posted it to biology, medical, and chemistry newsgroups! They
> | should know and care, because my question involves biological, medical,
> | and chemical entities!
>
> Did you ever post how you burned it?
> Was it some caustic soda?
> :)

No. I've said repeatedly, the burn was caused by contact with a hot
plate.

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T17:08:44Z

"Radium" <glucegen1@...> wrote in message
news:1138840980.823758.232290@......
| tressure@... wrote:
| WTF?! I posted it to biology, medical, and chemistry newsgroups! They
| should know and care, because my question involves biological, medical,
| and chemical entities!

Did you ever post how you burned it?
Was it some caustic soda?
:)

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T16:43:00Z

tressure@... wrote:
> > Well, no one gave me any rational answer.
>
> This would be conclusive evidence of one of two things:
>
> a) Nobody here knows.
> b) Nobody here cares.

WTF?! I posted it to biology, medical, and chemistry newsgroups! They
should know and care, because my question involves biological, medical,
and chemical entities!

> Either way, continuing to ask the same question again and again is
> unlikely to generate new and interesting responses.

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T16:12:36Z

As you are obviously pretty new I will go easy on you:

Welcome to usenet! Drop it already.

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T16:07:52Z

> Well, no one gave me any rational answer.

This would be conclusive evidence of one of two things:

a) Nobody here knows.
b) Nobody here cares.

Either way, continuing to ask the same question again and again is
unlikely to generate new and interesting responses.

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T15:44:05Z

Nichevo wrote:
> This has been reposted more than enough already, do it again and post
> photo evidence or fuck off.

Well, no one gave me any rational answer. Some told me it was
vasoconstriction. Others said it was dehydration. I know it was neither
vasoconstriction or dehydration. However, I *still* don't know exactly
what caused the thermal burn to look white.

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Re: Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T15:29:01Z

This has been reposted more than enough already, do it again and post
photo evidence or fuck off.

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Why was the FIRST-degree burn ^^^^FOAMY WHITE^^^^?!?! Was It Coagulation??!!?!?!?!?!?!?!?! Please Answer With Reasonable Responses!!!! Its NOT VASOCONSTRICTION or DEHYDRATION!!!!!!!!!!

2006-02-01T15:07:43Z

Why has no one answered this question of mine? Please answer it

bara...@... wrote:
> The redness and whiteness during light burns is the result of different
> behaviour of the subcutaneous capillaries.

No. Redness and PALENESS is the result of the different behavior of
capillaries.

There is a difference between paleness and whiteness.

> Heat induces shock.
>
> The resident granulocytes (histiocytes residing in the subcutaneous tissue)
> degranulate, and among others release histamine that is a potent
> vasoconstrictor. It causes the smooth muscle in the capillary to constrict.
> Constricted blood vessels reduce the blood flow locally, and the skin looks
> pale (white).

>This reaction is less visible in the more pigmented skin.

Not in my case. I am very dark-skinned [literally as dark as an oreo
cookie]. My burn wound appeared white -- foamy white. It was NOT cold,
so vasocontriction didn't cause the color change. The wound was
unusually hot, extremely irritating and painful and FOAMY WHITE.

What caused this whiteness? I know it is NOT vasoconstriction! It
seemed like a first-degree burn. The skin texture and moisture content
didn't change much. There was NO _swelling_ at all!

Is it the coagulation of the epidermal proteins that causes the
'whiteness'? If not, then what causes
it????????????????????????????????!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?!?

I know for a fact that it is ^^^^NOT^^^^
vasoconstriction!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

I know for a fact that it is ^^^^NOT^^^^
^^dehydration^^!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

The plate wasn't NEARLY hot enough to ^^dehydrate^^ my skin AT ALL.
Plus the duration of contact wasn't long enough to dehydrate my skin in
the burn region.

I hope my response didn't seem harsh. I was just trying to clarify that
the WHITENESS I am describing has nothing to do with VASOCONSTRICTION.

Unlike what you describe, the WHITENESS did NOT turn red at all. The
wound began to darken to my normal skin color after it healed.

I hope you understand.

> Now if the burn is more severe, is causes tissue damage. Cells necrotize and
> the permeability of endothelial lining of the blood vessels increases
> (serum exudes) in addition to the histamines other components are released
> too including the components of the complement system, all of this results
> in a localized, but rapidly escalating inflammatory reaction. This further
> increases the vascular permeability, vasodilation and enhanced blood which
> is necessary for leucocytes to infiltrate the damaged tissue (to protect and
> help to repair it).
>
> At this stage erythema formation is common occurrence which is the swelling
> and redness of the affected areas of the skin. This takes some time to
> develop, so the proper order of changes is: whiteness immediately after the
> injury, followed by redness in minutes or hours.
>
> Now if you have a VERY strong burn, "cauterizing" burn, that coagulates
> proteins even deeper in the tissue, it plugs the blood vessels by causing
> the proteins to coagulate, there is no blood circulation, and the skin
> remains pale (white). Because of reduced circulation, it takes much longer
> for the inflammatory reaction to begin and usually the injury develops a
> decently sized "sack" filled with yellowish plasma that hides any color
> below it (plain optical phenomenon) it protects the underlying tissue but
> dries up gradually and normal blood coagulation proteins help to form a
> brownish cover over the damaged area.
>
> The ultimate burn injury results in charring. Charred tissue is initially a
> dry mixture of skin proteins and blood, contains large amount of
> carbohydrates, lipids, hemoglobins etc, and all kind of degradation products
> thereof, carbonized, caramelized sugars etc, that causes it to have a dark
> almost black color. It never regenerates (dead tissue), but easily breaks
> and allows bacteria to seep into the wounds. charred tissue is usually
> removed to hasten the healing.
>
> lajos

Hmm, I wonder what would happen if all the proteins in my epidermal
tissues throughout my body were thermally-denatured completely but did
NOT form *covalent* bonds after being denatured.

Please note: My email address is not glucegen1@.... I don't use
that address. I use glucegen1b@...

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)Nj ***Hot stuff - check this out !!! )Nj

2006-01-28T09:37:32Z

http://www.kaneva.com/checkout/stream.aspx?assetId=2017&free=0

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