Nabble - Bio.net - Immuno

Most cited research reagents and kits

2009-12-02T23:54:40Z

An interactive graph showing most cited research reagents and kits
that are
currently available in the market. The data is based on recent
published journal articles, patents and patent applications. The
current research reagent/kits listings are: Transfection reagents, RT-
PCR, Real-time PCR, RNA purification, cAMP assays, Cytotoxicity assays
and Western blot reagents. Please visit:

http://www.sciclips.com/sciclips/most-cited-reagents.do?cat=TransfectionReagents\
&catId=1501
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Converting pmol/L to pg/mL for sCTX I?

2009-11-20T00:47:46Z

Dear Dr.Davis,

I would like to know how to convert serum CTX-I levels from pmol/l to
ng/ml? I understand how to calculate it, but I do not know the molecular
weight of serum CTX.

Would you be so kind to give advice?

Thank you for your help.

Ivana Sulkova, MD.

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Re: Immuno Digest, Vol 51, Issue 1

2009-11-19T12:43:36Z

Hi,

I don't use such kind of device, but I suppose that it can be use with PC interface. In this situation will be more easy to use the photometer. For this purpose, you must install a software in the PC. The vendor must assist you. Also, you can contact the manufacturer.

Adrian Ardelean
Romania

--- On Thu, 11/19/09, immuno-request@... <immuno-request@...> wrote:

From: immuno-request@... <immuno-request@...>
Subject: Immuno Digest, Vol 51, Issue 1
To: immuno@...
Date: Thursday, November 19, 2009, 7:04 PM

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Today's Topics:

   1. natural mouse TNF-alpha (Nyabuto, Evans (ILRI))

----------------------------------------------------------------------

Message: 1
Date: Wed, 18 Nov 2009 08:54:21 +0300
From: "Nyabuto, Evans (ILRI)" <E.NYABUTO@...>
Subject: [Immunology] natural mouse TNF-alpha
To: <immuno@...>
Message-ID:
    <6BEABCD5CA640A44A848448A42A03B7306154DC5@...>
Content-Type: text/plain;    charset="us-ascii"

Hi!

I am Evans from International Livestock Research Institute Kenya.

I have a TITERTEK MULTISKAN MC Plate reader which its display is not
coming on.

May you assist me with its manual so that I may know how to solve its
problem.

Thanks in advance.

Evans.

------------------------------

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End of Immuno Digest, Vol 51, Issue 1
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natural mouse TNF-alpha

2009-11-17T21:54:21Z

Hi!

I am Evans from International Livestock Research Institute Kenya.

I have a TITERTEK MULTISKAN MC Plate reader which its display is not
coming on.

May you assist me with its manual so that I may know how to solve its
problem.

Thanks in advance.

Evans.

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New Flow Cytometry software for ACS 1.0

2009-10-11T11:19:25Z

Hi,

I have developed a new flow cytometry program that can read a partial
list of the ACS 1.0 standard. The program can process parByte, int32,
and parShort list mode files. I have made it open and posted it on:

https://sourceforge.net/projects/kflowcacs/

Let me know what you think.

Diana
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Immunology Researchers: Access to over 10, 000 researchers from 95 countries and advanced research tools

2009-10-06T10:31:04Z

Dear Immunology Researcher,

I recently launched the research website, www.MyNetResearch.com. It
has since grown to over 10,000 members from 95 countries, who work on
their research projects, use specialist tools, and collaborate with
others from across the world. As a member of MyNetResearch.com, you
can:

• manage your research projects more effectively
• collaborate with thousands of other researchers on your research
papers
• conduct online surveys and citation searches
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• read about the latest research in hundreds of different subjects

I’m trying to let researchers know about this important resource and
new way of conducting research. Regular membership is completely,
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at:

https://www.mynetresearch.com/Signup/SignUp.aspx

Thanks,

Bay Arinze, Ph.D
Editor
MyNetResearch,
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Re: Immuno Digest, Vol 50, Issue 1

2009-10-02T13:47:46Z

Dear Eliza,
What do you want to convert. It have a large variable related with substrat_______________________________________________
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Request

2009-10-01T10:10:37Z

Hello,

could you help me convert 2.0 ng/l into pmol/L please?

It regards the blood test (very important to me).

Thanks in advance,
Eliza K.

----------------------------------------------------
Pokaż swojej dziewczynie zdjęcia z tym,
co robiłeś z kumplami na wakacjach:
http://klik.wp.pl/?adr=http%3A%2F%2Fpikeo.pl&sid=875

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BIT Life Sciences' 1st Annual World Congress of Immunodiseases and Therapy 2010 (WCIT-2010)

2009-09-27T19:01:01Z

WCIT is a focused conference to present and deliberate the advances in immunology, which is significant, timely and important to the health of humanity. The well selected topics cover across disciplinary breaking, from concept to therapy. It is anticipated over 500 global specialists to get together to present and share their cutting-edge technologies and up-to-date researches in an innovative and invigorating combination of presentations, symposia, discussions, panel discussion sessions and associated young scientist poster sessions along with exhibition of technology and new products. The comprehensive and intellectually stimulating scientific program of the first annual will embrace a broad spectrum of topics in basic science, medical research, diagnosis, prevention, clinical treatment and therapy of immune diseases. We believe that WCIT-2010 will definitely provide you an ideal platform for promoting ground breaking research and seeding potential collaborations for relevant parties of tailored interests not only in R & D areas but also the industrial and medical sectors.
The Initiative of WCIT-2010 will include both oral and poster presentations from academic and industrial settings. We hope to set a stage for all the medical doctors, the academicals, the clinical and the industrial researchers to make your research and innovations known with equal opportunity, means to join this event actively.
WCIT+2010-Invitation+Letter.doc

cell surface markers for THP-1 cells

2009-09-24T08:35:39Z

Hi,

I am currently working with THP-1 cells (ATCC TIB 202). I mainly work with this cells without differentiating them. I want to do some cell surface expression analysis of these cells using Flow Cytometry, and I am trying to find some good positive controls to perform the necessary compensation in flow cytometry.

Most of the literature I have found on flow cytometry conducted on THP-1 cells is performed on THP-1 cells which have been differentiated with either PMA or Vitamin D. And this differentiation causes a tremendous difference in the expression profile of THP-1 cells, especially CD14 which is not expressed in undifferentiated THP-1 cells.

I am wondering whether anybody can suggest a good marker for undifferentiated THP-1 cells.

Thanks.

Calvin.

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Submit Your Research Papers to be Read and Cited by Thousands of Researchers

2009-09-05T08:53:36Z

Dear Immunology Researcher,

Do you have working papers or conference articles that are not
available in online bibliographic databases but which you'd like to
share?

I invite you to upload your academic papers to the #1 research portal
– MyNetResearch.com. Over 9,500 members from all over the world will
be able to read and cite your work.

Store, retrieve, and share your articles with the largest and best-
known research community in the world. Search our new repository of
papers by subject area and keywords to find articles describing what
other researchers are doing in your area.

Join MyNetResearch for free and submit your articles today. Join at:
https://www.mynetresearch.com/Signup/SignUp.aspx

Sincerely,

Bay Arinze, Ph.D., Senior Editor
MyNetResearch.com
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Introduction to Bioinformatics, October 2009

2009-08-24T23:55:45Z

The online course, Introduction to Bioinformatics, is designed to
provide professional development for biologists working in a wide range
of different areas. For example, it would be suitable for anyone with
an interest in immunological bioinformatics or systems immunology.
Students successfully completing the assessments will receive a
certificate from The University of Manchester.

As for all our distance courses, teaching is delivered in a Virtual
Learning Environment that allows us to extend the classroom into the
web. We use a range of tools to support our online teaching activities.

Teaching and learning are focussed around tutor-supported exercises. In
this course, participants work together on the interpretation of their
results, before receiving feedback from the course tutor.

Week 1 Introduction to distance learning
Bioinformatics as a knowledge-based discipline

Weeks 2 and 3 Introduction to the sequence databases
Quality, redundancy and annotation
Sequence retrieval exercise and group discussion

Weeks 4 and 5 Nucleic acid sequence analysis

Weeks 6 and 7 Independent research and assessment

Weeks 8 and 9 Sequence alignment and database search

Weeks 10 and 11 Protein function prediction.

Weeks 12 and 13 Protein structure prediction
Practical exercise and group discussion

Weeks 14 to 16 Independent research for the second assessment

You will find information on all our courses, including fees and a link
to the online application form, here :
http://octette.cs.man.ac.uk/bioinformatics/index.html If you have any
questions, or need advice on the module options, please contact
Heather.Vincent from manchester.ac.uk

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cross-species single primary cell immunoglobulin production rates?

2009-07-21T05:10:05Z

I'm helping a modeler try to tackle a hairy vaccine dose prediction
problem, and need to scare up some data. Several hours of lit searches
have yielded some, but not enough information.

Once I got past all the hybridoma and tumor cell production rate
papers, I found several old papers on antibody production rates in
various primary cell systems. Most of it was ex vivo, but little was
done to try to correlate that data with in vivo observation (either
tissue-specific rates or peripheral blood concentration). Also, there
is a broad range of estimates depending on the analysis done to make
those estimates (2000 molecules/cell/min to about seven times that).

Is anyone aware if there are direct comparisons done on rodent v.
primate Ab production rates? In other words, is there a reason to
think that a maximally stimulated rodent plasma cell would produce
dramatically more or less IgM or IgG than a comparable human cell?
Thanks

v_a_x_j_o_b_s @ h_o_t_m_a_i_l_d_o_t_c_o_m_
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neutrophil isolation protocol

2009-07-14T12:55:47Z

Hello Bryan,

I read the protocol you posted online that describes how to isolate neutrophils from blood. Is it possible to use this protocol to isolate neutrophils from liver biopsies of people with chronic liver diseases?

Actually, I need to isolate neutrophils, DCs and Kupffer cells and I haven't found a protocol that I can use for the separation of the three types of cells. If you know a protocol that addresses this, I would greatly appreciate that information.

Thanks a lot,

Lorena Loarca
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searching for data on extinction coefficients for equine seric Fab, F(ab')2

2009-07-14T02:00:48Z

Dear all,

I would like to know about extinction coefficients for equine seric Fab,
F(ab')2.

Did you find it before?

How did you get it?

Thank you in advance

Susan

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hepatocytes and drug detoxification

2009-07-12T22:51:11Z

hi everybody,
any one working on hepatocyte culture and drug
detoxification? if yes we could discuss it thank you

ASHIS
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(no subject)

2009-06-17T12:17:20Z

Hi, I am Dilek Tiyekli from Trakya University in Turkey. I am master student at Immunology division.

I need some information about normal cytokine level in adults. Specially, IL1,IL6,IL10 and TNF alfa.

And ı need these cytokınes' half-life, diurnal rythm.

How long does it take until cytokine levels turn back normal after an infectious or inflamatory process?

Thanks everyone.

_________________________________________________________________
Teker teker mi, yoksa hepsi birden mi? Arkadaşlarınızla ilgili güncel bilgileri tek bir yerden edinin.
http://www.microsoft.com/turkiye/windows/windowslive/products/social-network-connector.aspx
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mpo

2009-06-16T01:01:06Z

Hello,
i am Esther Peltenburg from the Netherlands. I am working on a research lab and busy with myeloperoxidase assay.
We have some problems with reproduction of the results. I hope maybe you can help me by sending your methods for the assay and some results.

Best regards,

Esther

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fluorescent ELISA

2009-05-29T02:32:09Z

Hallo,
I would like to perform competitive ELISA with fluorescent labelled
antibody fragment (VHH exactly). Have anybody done such assays? Could you
provide me some tips which fluorescent dye should I use and which ELISA
plate to use - I would prefer stereptavidin coated, but have no idea if
they are suitable for fluorescent assays?
Will be grateful for some hints.
Magda

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Re: Immuno Digest, Vol 45, Issue 5

2009-05-20T22:36:14Z

Hi Snehal Mhatre,

You can use lactate dehydrogenase assay (colorimetric assay) to detect the NKCA.

regards,
Lit

--- On Thu, 21/5/09, immuno-request@... <immuno-request@...> wrote:

From: immuno-request@... <immuno-request@...>
Subject: Immuno Digest, Vol 45, Issue 5
To: immuno@...
Date: Thursday, 21 May, 2009, 1:07 AM

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Today's Topics:

   1. Non radioactive Cytotoxic activity assay (snehal mhatre)

----------------------------------------------------------------------

Message: 1
Date: Wed, 20 May 2009 21:00:30 +0530
From: snehal mhatre <snehalrm@...>
Subject: [Immunology] Non radioactive Cytotoxic activity assay
To: immuno@...
Message-ID:
    <36383dd50905200830n6be6b5f3sc206828d81b020c6@...>
Content-Type: text/plain; charset=ISO-8859-1

I am Snehal Mhatre, PhD student at IIH, Parel (Mumbai, India). I have to
work on the activity of NK cells and I am looking out for a
non-radioactivity method. Can you help me out me in this? Iwould like to
know the different methods which I can use and also the reproducibility of
the methods.

thank you

------------------------------

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End of Immuno Digest, Vol 45, Issue 5
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Non radioactive Cytotoxic activity assay

2009-05-20T08:30:30Z

I am Snehal Mhatre, PhD student at IIH, Parel (Mumbai, India). I have to
work on the activity of NK cells and I am looking out for a
non-radioactivity method. Can you help me out me in this? Iwould like to
know the different methods which I can use and also the reproducibility of
the methods.

thank you
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CD56CD16 bright, CD56CD16 dim

2009-05-14T19:06:01Z

We have a client who wishes to do a dot plot with CD56PE CD16PE on the y
axis vs CD3 on x axis. The client wants to separate CD56CD16 bright cells
from CD56CD16 dim cells. We have run some normal patients and found that
only one population of cells shows up. There is no clear delineation
between a bright and dim population for CD56CD16 staining. Has anyone done
this before and does anyone know how to set the gate so that one could
delineate bright and dim cells?

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Memorandum of Understanding for Confrence "Acapulco and Hawaii 2009 Immunology Conferences"

2009-05-12T21:33:00Z

Dear Sir/Madam,

We are pleased to inform you that "American Journal of Immunology" decided to dedicate a special issue for publication of all accepted articles presented at your conference for more citation and world wide readership.

American Journal of Immunology is available in both, online and print format with full length articles for free and unlimited data download.

American Journal of Immunology would bear printing and publication cost of all articles.

Would you please kind enough to spare some time to respond and forward this e-mail, add our name in sponsors list and share your ideas with us.

I look forward to hearing from you in very near future.

Regards

Editor
Science Publications
http://scipub.org

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RE: Immuno Digest, Vol 45, Issue 1

2009-05-07T20:38:18Z

Hi,

There are many anticogulants e.g haparin, ACD and EDTA are few of them.Usage of ACD/EDTA/heparin actually depends what you want to do with isolated neutrophiles.

In our experience of DNA typing, we are using ACD instead of EDTA. Acid Citrate Dextros has advantage especially in DNA amplification (PCR reactions) as EDTA cause amplification problem in amplification, and Heparin is expensive.

with regards

Raza Ali

Scientific research officer

SIUT.

> Date: Thu, 7 May 2009 12:04:00 -0500
> From: immuno-request@...
> Subject: Immuno Digest, Vol 45, Issue 1
> To: immuno@...
>
> Send Immuno mailing list submissions to
> immuno@...
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://www.bio.net/biomail/listinfo/immuno
> or, via email, send a message with subject or body 'help' to
> immuno-request@...
>
> You can reach the person managing the list at
> immuno-owner@...
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Immuno digest..."
>
>
> Today's Topics:
>
> 1. Human Peripheral blood's Neutrophil isolation (Faimola Guerrero)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 06 May 2009 12:25:02 -0500
> From: Faimola Guerrero <fguerrero@...>
> Subject: [Immunology] Human Peripheral blood's Neutrophil isolation
> To: <immuno@...>
> Message-ID: <C627321E.A0%fguerrero@...>
> Content-Type: text/plain; charset="US-ASCII"
>
> Hi I am trying to use your protocol to isolate neutrophils. What is the
> purpose of the ACD is it a substitute for heparin?
>
> --
> Faimola Guerrero
>
>
>
> ------------------------------
>
> _______________________________________________
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>
> End of Immuno Digest, Vol 45, Issue 1
> *************************************

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Human Peripheral blood's Neutrophil isolation

2009-05-06T10:25:02Z

Hi I am trying to use your protocol to isolate neutrophils. What is the
purpose of the ACD is it a substitute for heparin?

--
Faimola Guerrero

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help with specific ICS protocol

2009-04-22T08:28:36Z

Hello All,

I need to do some staining for mouse CD4, IFN-g, IL-17, and CCR6. I haven't
tried this combination before and have difficulty doing ICS in general (loss
of surface stains). Would anybody have any experience on a good clone and
fluor combination for these markers. I am really grateful for any help.
Thank you.

Justine Tigno-Aranjuez
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indirect ELISA protocol

2009-04-22T05:32:31Z

Dear all,
I am working on plant derived vaccines. I would like to know whether an
indirect ELISA can be standardized to quantify the amount of antigen
expressed in plants. Kindly let me know if any protocol is available.

Sandesh Rao,
Senior Research Fellow,
Plant vaccine Lab,
UAS, B'lore,India. - 560065.
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Ceramide ELISA

2009-04-21T09:24:50Z

Dear Henk,

I am very interested in the Ceramide ELISA. Did you develop the ELISA kit? Is it available in your lab or somewhere else?

I appreciate your guide about it.

Thank you!

Regards,

Xin Li, Ph.D.
Myeloma Institute for Research and Therapy
Winthrop P. Rockefeller Cancer Institute, Room 938
University of Arkansas for Medical Sciences
4301 W. Markham, Slot 776
Little Rock, Arkansas 72205
Tel: (501) 296-1503 ext. 1461
Fax: (501) 686-6442
E-mail: lixin@...<mailto:yaccobyshmuel@...>

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bacterias 41(DE3) e C43(DE3)

2009-04-20T15:12:05Z

Hi,

I am needing the C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS (E.
coli bacterias)
Does anybody have these bacterias?
//Can anybody help me?
Thank you very much,

**C41(DE3), C41(DE3)pLysS and C43(DE3), C43(DE3)pLysS
E. coli BL21(DE3) strains, like Lucigen's E. cloni EXPRESS Competent
Cells provide reliable expression of many proteins cloned into T7
expression vectors (e.g., pET or Lucigen's pSMART®-cDNA vectors).
However, in some cases expression is minimal or not detectable because
the recombinant protein, when expressed, is deleterious or lethal to
these standard BL21 strains. Examples of such toxic proteins include
many membrane proteins, some cytoplasmic proteins, and nucleases.
Unfortunately, successful expression of one or more toxic proteins is
often important to the experimental goal.

http://www.lucigen.com/catalog/index.php?cPath=47_58

sincerely

--
Itabajara da Silva Vaz Junior
Centro de Biotecnologia - UFRGS
C.P. 15005
Av. Bento Goncalves 9500
Predio 43421 - Campus do Vale
91501-970 Porto Alegre RS Brasil
Fone (51) 33086078 Fax (51)3308-73-09 [00 55 51 3308 7309]
http://www.ufrgs.br/depbiot/201/cbt201.htm
http://www.ufrgs.br/favet/imunovet

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RE: ELISA protocol

2009-04-17T14:50:17Z

Dear Dr Sudheesh

You may use both but the second one is better since some inespecific
stimulation can slightly increase the background in the ELISA. The best
is to have a group of sera and to use the mean and the STD to calculate
the cut off.

regards

Carlos Duarte

-----Original Message-----
From: immuno-bounces@...
[mailto:immuno-bounces@...] On Behalf Of m s sudheesh
Sent: Friday, April 17, 2009 1:27 PM
To: immuno@...
Subject: [Immunology] ELISA protocol

Dear All,
I am working on the development of nanoparticulate vaccine. While
performing ELISA potocol the end point titre was expressed as the
logarithm of the reciprocal of the last dilution which gave an optical
density at 490nm above the optical density of negative control. What
should be the negative control is it preimmune sera or the sera of
animals trated with blank nanoparticles or the well containing only
substrate. Kindly help me.

M.S.Sudheesh,Research Fellow,
Pharm Biotech Div, Department of Pharmaceutical Sciences, Dr. H.S.Gour
University, Sagar, M.P,India,470001 Phone +91-9826415402
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ELISA protocol

2009-04-17T10:26:37Z

Dear All,
I am working on the development of nanoparticulate vaccine. While performing ELISA potocol the end point titre was expressed as the logarithm of the reciprocal of the last dilution which gave an optical density at 490nm above the optical density of negative control. What should be the negative control is it preimmune sera or the sera of animals trated with blank nanoparticles or the well containing only substrate. Kindly help me.

M.S.Sudheesh,Research Fellow,
Pharm Biotech Div, Department of Pharmaceutical Sciences, Dr. H.S.Gour University, Sagar, M.P,India,470001
Phone +91-9826415402
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Plasma to serum protocols?

2009-04-15T08:14:05Z

Dear Sir,
I need this protocol. Please help me.
Thanks and best regards,
Dr Hemender Kumar
Manager QA/QC
Trinitron Biotech Limited,
Abuja, Nigeria
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A Useful Website for Immunology Researchers - MyNetResearch.com

2009-04-09T19:20:42Z

Dear Immunology Researcher,

With a team of academics, I recently launched the research website,
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MyNetResearch,
Empowering Collaboration™
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Q-dot

2009-03-26T12:29:23Z

I want to increase intensity of my Ab staining for flow. I'm using
anti-Rabbit FITC for my 2nd Ab. Has anyone found higher intensity with Q-dot
as a primary or Secondary Abs,could you let me know?
Thanks,
Min-Jung

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HyTest cardiac markers

2009-03-23T02:18:00Z

Hi,

HyTest offers immunological reagents for assay development and research
applications. The product portfolio includes reagents in such areas as
cardiac markers, infectious disease reagents, tumor markers, veterinary
reagents etc. Read more at http://www.hytest.fi/.

With best regards,

Katja Kivistö

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