Nabble - Bio.net - Microbio

Request :- Can anyone perform this experiment?

2009-12-11T06:05:38Z

Dear All,

Can anyone perform this experiment for me. I will add him/her as a co-author
to my paper on this experiment.

Details:-
---------------
Experiment to be carried out
Materials and Methods:
T-Antigen which is an antigen that requires the participation of T
lymphocytes even before an immune response could occur. As most antigens are
of this type, the antigen that requires the interaction between T and B
cells will be used to initiate antibody production. Viral stocks for the
analysis will be generated by transient transfection of 293T cells [Fouchier
RA et al 1997]. The virus will then be quantified by an enzyme-linked
immunosorbent assay (ELISA) for identifying the soluble concentration. In
order to generate high-titre viruses, the 293T cells can first transiently
be cotransfected with other virions [needs to be indentified and reviewed
carefully]. After infection, the input virus will be removed by extensive
washing with phosphate-buffered saline (PBS), and the T cells would be
incubated in fresh media for 36 hours to allow viral production to reach a
high level. The cells will then washed again with PBS and placed in the
appropriate medium for viral production. Further post-production, the
virus-containing supernatants would be centrifuged for 5 min at 500 x g and
filtered through 0.45-µm-pore-size filters. The infectivity of the viruses
will be determined by infection using logarithmic time scale. The T-Cells
within multicellular biological systems were infected with viruses in
different conditions and methods. There will also be several cases executed
based on which experiments will be conducted on cells. When an unplanned
event occurs with a cell such as pathogen infiltration, or high temperature,
etc then a cell is able to respond in a particular behaviour. Here we will
compare the human body (multi-cellular organism) and the cell (single cell
of human body) with their behaviour capabilities & try to answer the
questions in our work favourably. The behavioural aspects of a cell may also
be studied to understand more about them. Their vulnerabilities and coping
mechanisms could give us an idea of how cells resist diseases. In the
experiments mentioned in the methodology, the test cases may be observed or
improved to study the movements (or changes in the cell behaviour) in
different conditions. For example, how does a cell react when it is invaded
by a pathogen? what are the changes that happen to its structure and
functionality? Any such changes or irregularity can be studied here. We
believe comparing the test cases will reveal interesting observation about
the cell.

Reference:Fouchier, R. A., B. E. Meyer, J. H. Simon, U. Fischer, and M. H.
Malim. 1997. HIV-1 infection of non-dividing cells: evidence that the
amino-terminal basic region of the viral matrix protein is important for Gag
processing but not for post-entry nuclear import. EMBO J. 16:4531-4539
--------------

Regards,
Jitesh Dundas
+91-9561527190 / +91-9860925706
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: observing and counting bacteria within soft agar

2009-12-07T17:08:36Z

On 2009-12-07 14:05:19 -0500, Pavitra <pavitra.padmanabhan@...> said:

> Hi,
>
> Is there a simple way to count bacteria that are growing as a lawn in
> soft (top) agar? I'm working with E.coli. Would gram staining work?
>
> Thanks.

Your question shows that you don't quite understand the basics of
microbiology cultures. There are many methods of counting bacteria,
some of which are counting colonies on agar, others require developing
a growth curve with a spectrophotometer.

You need to go back to your basic micro book and look up the topic of
colony counts. Learn how to make serial dilutions in a broth that will
support your bacteria and then how to plate a small enough aliquot to
give you countable colonies. Then you can calculate your bacterial
count per ml or what ever you are trying to count.

By the way, a "lawn" of growth refers to a confluent growth across the
whole plate, and not countable discrete colonies.
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
yjgent@...

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

observing and counting bacteria within soft agar

2009-12-07T11:05:19Z

Hi,

Is there a simple way to count bacteria that are growing as a lawn in
soft (top) agar? I'm working with E.coli. Would gram staining work?

Thanks.

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Question on Enumeration of PAH degrading bacteria

2009-12-06T04:23:49Z

Greetings!

Enumeration of PAH degrading bacteria can be based on turbidity in spectrophotometry readings. But how to avoid any turbidity related to PAH itself?
My readings are not consistent and when there is high turbidty,basically i think its because of the oil that has been pipetted together during sampling from flask. Moreover, the use of blank here doesnt really solve the problem for me to assume there is no interference from oil.

Secondly, can a free floating powder medium eg bushnell haas, that are not 100% dissolved can interrupt the turbidity also?

Hope to get a hand!

Cheers,Shalini.
_________________________________________________________________
New Windows 7: Simplify what you do everyday. Find the right PC for you.
http://windows.microsoft.com/shop_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

16s rRNA sequencing-doubt

2009-12-04T03:40:12Z

Devi,
Try a tree-based method such as http://www.hpa-bioinfotools.org.uk/sequence_entry_16s_MAFFT_frame.html. It could help clarify things.

Best wishes,
Vince

Vincent Mulholland
Molecular Biology Unit Manager - Diagnostics & Molecular Biology Section
Science and Advice for Scottish Agriculture (SASA)

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

16s rRNA sequencing-doubt

2009-12-03T03:39:26Z

hi every one

i did sequence bacteial 16s rRNA gene for identification
manually assembled the full length sequence and blasted in NCBI website.

i did it this sequence for two bacterial sample

1.
in one sample the blast result shown 100 number of similar bacteria name and
all of these have 98% identity with the query.
if i see species there is around 4 species which occurs repeatedly among 100

i have doubt which bacteria is mine query belongs to
i thought of doing biochemical test to confirm
but all are showing 98% so, which one to select???

2.
in seecond sample all blast result showing 93% identity
so, here also i have doubt
whether i can consider this 93% identity or not??
if i consider which one i can select???

can anyone help me???

thank u so much

devi

--
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Brownian motion

2009-12-02T18:14:36Z

Dear Dr Alan J. Cann,

Could you tell me some information about the Brownian motion video (rate of frames, dimension of image, ...)? I would like to do some homework about that video with my students, and I need these informations.

Thank you very much.

Stéphane Houle
Coordonnateur du département de physique
Collège Jean-de-Brébeuf
Tél: 514-342-1320 #5462
Courriel: Stephane.Houle@...

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Microbio Digest, Vol 55, Issue 1

2009-12-02T10:06:23Z

You could try 16S rDNA sequencing from PCR products. There are also
commercial labs that will do that as well, such as MIDI Labs
(http://www.midilabs.com/).

Good luck

-----------------------------------------------
Eric T. Gillock, Ph.D.
Associate Professor
Department of Biological Sciences
Fort Hays State University
Hays, KS 67601
-----------------------------------------------

From: microbio-request@...

To: microbio@...

Date: 12/02/2009 11:06 AM

Subject: Microbio Digest, Vol 55, Issue 1

Sent by: microbio-bounces@...

Send Microbio mailing list submissions to
microbio@...

To subscribe or unsubscribe via the World Wide Web, visit
http://www.bio.net/biomail/listinfo/microbio
or, via email, send a message with subject or body 'help' to
microbio-request@...

You can reach the person managing the list at
microbio-owner@...

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Microbio digest..."

Today's Topics:

1. Info on ID (Krishna Sharath)
2. Seeking open innovation ideas in malaria and tuberculosis
(Sciclips)

----------------------------------------------------------------------

Message: 1
Date: Tue, 1 Dec 2009 14:04:30 -0500
From: "Krishna Sharath" <SKrishna@...>
Subject: [Microbiology] Info on ID
To: "microbio@..." <microbio@...>
Message-ID:
<B7FF8D4C259B5442BCC354A568C0FD791024C9E29B@informa-jhpvcrb>
Content-Type: text/plain; charset="us-ascii"

I have found bacteria in very toxic body fluids of large animals, but could
not identify them. Can anyone help me with ID ing these bacteria? May be
using molecular techniques.

S. Krishna

------------------------------

Message: 2
Date: Tue, 1 Dec 2009 16:46:11 -0800 (PST)
From: Sciclips <kar.sanchayita@...>
Subject: [Microbiology] Seeking open innovation ideas in malaria and
tuberculosis
To: microbio@...
Message-ID:

<c3e643fb-54a7-490b-8d70-582b27eed83a@...>
Content-Type: text/plain; charset=windows-1252

Sciclips is seeking open innovation ideas in mosquito-borne diseases
(like malaria) and tuberculosis. This is a unique opportunity for all
scientists world-wide, irrespective of their filed of research, to
contribute multidisciplinary ideas to find cure for malaria and
tuberculosis. We call this opportunity as "Scientific Philanthropism”:
donate your ideas and help millions of people. Please visit
www.sciclips.com
to see currently posted open innovation ideas.

------------------------------

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

End of Microbio Digest, Vol 55, Issue 1
***************************************

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Seeking open innovation ideas in malaria and tuberculosis

2009-12-01T16:46:11Z

Sciclips is seeking open innovation ideas in mosquito-borne diseases
(like malaria) and tuberculosis. This is a unique opportunity for all
scientists world-wide, irrespective of their filed of research, to
contribute multidisciplinary ideas to find cure for malaria and
tuberculosis. We call this opportunity as "Scientific Philanthropism”:
donate your ideas and help millions of people. Please visit www.sciclips.com
to see currently posted open innovation ideas.
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Info on ID

2009-12-01T11:04:30Z

I have found bacteria in very toxic body fluids of large animals, but could not identify them. Can anyone help me with ID ing these bacteria? May be using molecular techniques.

S. Krishna
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: question

2009-11-23T14:42:56Z

Shalini selvam wrote:
>
> Hello there!
>
> Thank you Mr John and Bob for the previous reply.
>
> Regarding the question on how to construct microbial consortia, im trying to
> construct microbial consortia for bioremediation of hydrocarbon
> contaminated soil. All the papers that i came across grew the inoculum
> separately and then combined them.

What microbes comprise your consortium? Or rather, which are
you planning to use? Contrary to popular belief, microbes aren't
plug-and-play, things like competition for e- donors/acceptors
comes into play once you leave the realm of the Pure Culture...
there's considerably more to the process than simply bunging
bugs in a bottle and awaiting results.

> Can we combine them directly and then test for the abiltiy of bacteria to degrade oil ? Eg lets
> say flask 1 contains 1ml oil+100ml media+2ml inoculum of one type and
> flask 2 contains 1ml oil+100ml media+ 1ml each of 2 different inoculum
> for the testing?

Hydrocarbons (which includes the volatile organics) in general
or oil, in particular? And if only oil, then what sort of oil? Crude,
fuel, clean, dirty, high or low sulphur content?

IMO, the experiment you propose has a lot of variables which have
to be wrangled into submission before you'll get anything like a
reproducible result. I wish you the best, however.

Deirdre

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

question

2009-11-23T01:15:58Z

Hello there!

Thank you Mr John and Bob for the previous reply.

Regarding the question on how to construct microbial consortia, im trying to
construct microbial consortia for bioremediation of hydrocarbon
contaminated soil. All the papers that i came across grew the inoculum
separately and then combined them.

Can we combine them directly and then test for the abiltiy of bacteria to degrade oil ? Eg lets
say flask 1 contains 1ml oil+100ml media+2ml inoculum of one type and
flask 2 contains 1ml oil+100ml media+ 1ml each of 2 different inoculum
for the testing?

Thank you!

Sincerely,Shalini

_________________________________________________________________
New Windows 7: Simplify what you do everyday. Find the right PC for you.
http://windows.microsoft.com/shop_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: question

2009-11-22T18:16:51Z

On 2009-11-22 10:37:25 -0500, Shalini selvam <lovey5dovey@...> said:

>
> Hello there!To anyone concern:
>
> I have a question.
>
> How long can a media last after being autoclaved and where should we store
> it?can we autoclave it twice?
>
> And how do we set up microbial consortia?
>
> Thank.
>
> Regards,Shalini
>
> _________________________________________________________________
> Windows 7: Simplify what you do everyday. Find the right PC for you.
> http://windows.microsoft.com/shop

Generally media that has been dispensed into screw capped tubes will
last a lot longer, especially if the caps are screwed tight after the
media has cooled. Petri dish media dries out and needs to be sealed in
a bag or container and usually requires refrigeration.
If you add blood to a media, it will shorten the "expriation date"
significantly. I wouldn't trust blood media past 4 to 6 weeks.
If you make the media in large flasks, you must cool the agar to about
50C and then pour into whaterver vial or plate you intend to use. It is
not recommended to autoclave again since that could carmalize some of
the sugars and may change some chemical properties and pH. You can
remelt media by bringing it above the melting temp of agar which is
around 97C, but don't go over that, just enough to get it all to melt.
There are a few good references for media preparation including the
Difco Manual and the American Society of Micribiology Manual of
Clinical Micribiology. These are the references I've used in my many
years of media making and storing.

--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
yjgent@...

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

question

2009-11-22T07:37:25Z

Hello there!To anyone concern:

I have a question.

How long can a media last after being autoclaved and where should we store it?can we autoclave it twice?

And how do we set up microbial consortia?

Thank.

Regards,Shalini

_________________________________________________________________
Windows 7: Simplify what you do everyday. Find the right PC for you.
http://windows.microsoft.com/shop_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Request For A partner on my projects

2009-11-16T23:25:15Z

Dear Sir/Madam,

I am looking for a research partner who could help me perform wet lab
experiments. I do not have access to wet lab experiment equipments and that
is the only thing that stops me from completing my work. I have 2 papers
that need some biology inputs.

Please let me know if there is any enthusiastic and willing scientist who is
ready to help me in my work. I will be happy to make him my co-author in all
the research papers that are involved ( for the current two papers and other
papers in future too )

I look forward to a positive reply from some experts in this group.

Regards,
Jitesh Dundas
http://jiteshbdundas.blogspot.com
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

RE: constitutive promoters needed

2009-11-11T10:11:39Z

I do not know of a database but you may want to look at (for E.coli at least):
-Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning.
Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR.
Biotechniques. 2008 Sep;45(3):335-7.
-The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters.
Jensen PR, Hammer K.
Appl Environ Microbiol. 1998 Jan;64(1):82-7.
-Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering.
De Mey M, Maertens J, Lequeux GJ, Soetaert WK, Vandamme EJ.
BMC Biotechnol. 2007 Jun 18;7:34.

The first two papers include sequence info of the promoter sets.

Best,

Koen

-----Original Message-----
From: microbio-bounces@... [mailto:microbio-bounces@...] On Behalf Of Holger Merker
Sent: Wednesday, November 11, 2009 11:18 AM
To: microbio@...
Subject: [Microbiology] constitutive promoters needed

Dear all,

I am looking for constitutive prokaryotic promoters (especially for
E.coli, B.subtilis, Pseudomons fluorescens and putida, Acinetobacter)
differing in their transcriptional activity except for the lac-promoter
or the beta-lactamase promoter. I only found this webpage
(http://margalit.huji.ac.il/) but I would like to know whether the
specific promoter is a weak or strong promoter (beside the fact that it
is constitutive).
So, does anyone of you know a database containing such information? or
could you suggest me some further constitutive promoters with regard to
their transcriptional activity?

Any suggestion/hint/comment is welcome. Thx a lot for your help.

All the best, Holger

--
Dipl.Biol. Holger Merker

Max-Planck-Institut für Chemische Ökologie
Abteilung für Bioorganische Chemie (Prof. Boland)

Hans-Knöll-Strasse 8
07745 Jena
Germany

Tel.: +49-(0)3641-571212

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

constitutive promoters needed

2009-11-11T09:18:04Z

Dear all,

I am looking for constitutive prokaryotic promoters (especially for
E.coli, B.subtilis, Pseudomons fluorescens and putida, Acinetobacter)
differing in their transcriptional activity except for the lac-promoter
or the beta-lactamase promoter. I only found this webpage
(http://margalit.huji.ac.il/) but I would like to know whether the
specific promoter is a weak or strong promoter (beside the fact that it
is constitutive).
So, does anyone of you know a database containing such information? or
could you suggest me some further constitutive promoters with regard to
their transcriptional activity?

Any suggestion/hint/comment is welcome. Thx a lot for your help.

All the best, Holger

--
Dipl.Biol. Holger Merker

Max-Planck-Institut für Chemische Ökologie
Abteilung für Bioorganische Chemie (Prof. Boland)

Hans-Knöll-Strasse 8
07745 Jena
Germany

Tel.: +49-(0)3641-571212

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

acetobacter xylinum - where can I get it?

2009-11-11T06:29:09Z

Hannes,

Do not try buying, or otherwise obtaining, a bacterial culture if you do not have the experience or facilities
to grow it (responsible organisations would not give you a culture in any case). Find a local microbiologist
who can help you. He/she will know how to obtain this bacterium and will have access to the laboratory
facilities you need. A biologist at the LSE might know someone local who can help you.

Best wishes,

Vince

Vincent Mulholland
Molecular Biology Unit Manager - Diagnostics & Molecular Biology Section
Science and Advice for Scottish Agriculture (SASA)
SASA is a Division of the Scottish Government Rural Payments and Inspections Directorate

Correspondents should note that all communications to or from SASA (Science and Advice for Scottish Agriculture a Division of the Rural Payments and Inspections Directorate of the Scottish Government) may be automatically logged, monitored and/or recorded for lawful purposes.

The original of this email was scanned for viruses by the Government Secure Intranet (GSi) virus scanning service. On leaving the GSi this email was certified virus-free.

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: acetobacter xylinum - where can I get it?

2009-11-10T17:21:04Z

On 2009-11-10 10:30:15 -0500, <H.Steen-Thornhammar@...> said:

> Hi,
>
> My name is Hannes Steen Thornhammar and I am student at the London
> School of Economics. I am currently rsearching topics in sustainable
> futures and came across your name in regards to acetobacter xylinum.
> Would you be able to tell me how I can actually attain samples of the
> bacteria. Can I grow it myself? Can I buy it in the UK, London?
>
> Your assisstance in this matter would be greatly appreciated
>
> Best,
>
> Hannes
>
> Please access the attached hyperlink for an important electronic
> communications disclaimer:
> http://www.lse.ac.uk/collections/secretariat/legal/disclaimer.htm

Are you studying bacteriology or economics? Do you have any experience
in handling bacteria? Do you have access to laboratory facilities that
will allow you to manage a bacterial culture? Do you have a professor
who is mentoring you?

Handling bacteria can be a very dangerous proposition if not done
properly. Even so called non-pathogenic bacteria can cause some kind of
havoc if it gets into the wrong place.

There are places where you can purchase bacterial stocks, but you have
to be associated with a certified laboratory or educational facility
with proper labs to handle it. How are you planning to pay for it?

Lots of questions, hope you have a plan.
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
yjgent@...

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

acetobacter xylinum - where can I get it?

2009-11-10T07:30:15Z

Hi,

My name is Hannes Steen Thornhammar and I am student at the London School of Economics. I am currently rsearching topics in sustainable futures and came across your name in regards to acetobacter xylinum. Would you be able to tell me how I can actually attain samples of the bacteria. Can I grow it myself? Can I buy it in the UK, London?

Your assisstance in this matter would be greatly appreciated

Best,

Hannes

Please access the attached hyperlink for an important electronic communications disclaimer: http://www.lse.ac.uk/collections/secretariat/legal/disclaimer.htm

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

a question

2009-11-09T03:31:02Z

Dear colleagues
I appreciate if anybody can help me in the following problem:
I used Lpp'-ompA system (developed by Georgio et al., 1996) for surface display of a bacterial metallothioneine fused to chitin binding domain and 6Xhis-tag (on the surface of E.coli BL21). The length of this passenger protein is approximately 15kDa. We did metal binding assay using whole cell and we measured adsorption using a solution of cadmium Cd(NO3)2 in Tris-Hcl pH7.0. But it didn't show any adsorption. Hydropathy plot showed the passenger proteins are hydrophile and also it didn't show any transmembrane domain. I would like to know why does not this protein show any adsorption?
I appreciate your help.
Best Wishes
Reza

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Looking for Cell with unique Composition

2009-11-05T16:27:00Z

In article <hcvo89$stc$1@...>,
amdx <nojunk@...> wrote:
>Just so I'm clear, that is a single cell and the cilia are part of that
>cell.
>

Now, now, don't presume on my good nature.

--bks

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Looking for Cell with unique Composition

2009-11-05T15:48:30Z

"Bradley K. Sherman" <bks@...> wrote in message
news:hcvggu$s8i$1@......

> In article <hcvfdo$huh$1@...>,
> amdx <nojunk@...> wrote:
>>High School project, looking for something different.
>>Do you know of any cells that I can research to make
>>my project different than the usual.
>>Need to make a 3D model.
>> The Mav
>>
>>
>
> Surprisingly, each cell that exists, and has ever existed, has
> unique composition.
>
> Check out these cells:
> <http://www.microscope-microscope.org/applications/pond-critters/protozoans/ciliphora/euplotes.htm>
>
> --bks
>

Thanks Bradley,
That's neat, looks so much like an insect walking on that bubble.
Just so I'm clear, that is a single cell and the cilia are part of that
cell.
Many more on the site that I'll check out too.
Thanks,
The Mav

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Looking for Cell with unique Composition

2009-11-05T13:36:30Z

In article <hcvfdo$huh$1@...>,
amdx <nojunk@...> wrote:
>High School project, looking for something different.
>Do you know of any cells that I can research to make
>my project different than the usual.
>Need to make a 3D model.
> The Mav
>
>

Surprisingly, each cell that exists, and has ever existed, has
unique composition.

Check out these cells:
<http://www.microscope-microscope.org/applications/pond-critters/protozoans/ciliphora/euplotes.htm>

--bks

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Looking for Cell with unique Composition

2009-11-05T13:17:50Z

High School project, looking for something different.
Do you know of any cells that I can research to make
my project different than the usual.
Need to make a 3D model.
The Mav

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

inquiry about Aspergillus fumigatus and penicillium crustosum

2009-10-29T22:19:44Z

Dear All
hope you are fine and doing well
I want to ask if there is any record about using Aspergillus fumigatus and penicillium crustosum
in composting. if there is any reference please let me know
thanks
yours
Mohamed

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

New Academia.edu feature for Microbiology Usenet

2009-10-28T16:33:58Z

Dear Microbiology Usenet members,

I want to tell you about a new feature on Academia.edu that can
connect you with your mailing list. Academia.edu launched 12 months
ago and now helps 300,000 academics a month answer the question 'who's
researching what?'

And now we've built a dedicated page on Academia.edu for the
Microbiology Usenet mailing list:

http://lists.academia.edu/UsenetBionetMicrobiology

Here you'll learn about fellow members of Microbiology Usenet on
Academia.edu. You'll be able to see their research interests, papers,
and loads of other useful information.

Visit the link above, sign up with Academia.edu, and share your
research interests with fellow members of the Microbiology Usenet
mailing list.

Richard

Dr. Richard Price, post-doc, Philosophy Dept, Oxford University.
Founder of Academia.edu
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

USP Microbial Limits Prep Test

2009-10-21T07:40:11Z

Good Day,

I read your article and I have a question please? When you perform your validation test, for how long you keep your product in contact with microorganism in your diluents and why?

I appreciate your reply in advance.

Pharmascience Inc
Saeid Hashemi
Microbiology Analyst
shashemi@...<mailto:shashemi@...>

AVIS DE CONFIDENTIALITE :
Ce message est confidentiel et est a l'usage exclusif du destinataire. Toute autre personne est par la presente avisee qu'il lui est strictement interdit de le diffuser, distribuer ou reproduire. Si vous avez recu ce message par erreur, veuillez nous en aviser immediatement par retour de
courrier electronique et detruire ce message.

NOTICE OF CONFIDENTIALITY:
This communication is intended only for the party to whom it is addressed and contains confidential information. Any other delivery, distribution, copying or disclosure is strictly prohibited. If you have received this communication in error, please notify the sender immediately by return electronic mail and destroy this message.

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Microbio Digest, Vol 53, Issue 5

2009-10-07T15:04:01Z

Unlike my arrogant colleague?- I know contact plates are in current and common?use in hygienic manufacturing application and counts are related to the surface area of contact with the contact plates.? The data are quirte relevant to clean room hygiene.

No - as is obvious - there is no way to take TNTC? to a quantitation.??If you experience spreaders - you take the same precautions as in any culture - medium that diminshes, early counts, even overlay (tho you need a steady hand).

-----Original Message-----
From: microbio-request@...
To: microbio@...
Sent: Wed, Oct 7, 2009 1:08 pm
Subject: Microbio Digest, Vol 53, Issue 5

Send Microbio mailing list submissions to
microbio@...

To subscribe or unsubscribe via the World Wide Web, visit
http://www.bio.net/biomail/listinfo/microbio
or, via email, send a message with subject or body 'help' to
microbio-request@...

You can reach the person managing the list at
microbio-owner@...

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Microbio digest..."

Today's Topics:

1. Re: Rodac Plates (John Gentile)

----------------------------------------------------------------------

Message: 1
Date: Tue, 6 Oct 2009 19:49:53 -0400
From: John Gentile <yjgent@...>
Subject: [Microbiology] Re: Rodac Plates
To: microbio@...
Message-ID: <2009100619495316807-yjgent@nospamcoxnet>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

On 2009-10-05 11:41:10 -0400, "Cherie Parker"
<cherie_parker@...> said:

> We use Rodac (contact) plates for our environmental monitoring. Is there a
> method to estimate TNTC plates? How do you read spreader colonies on a
> contact plate? Is there a standard method for counting colonies on a Rodac
> plate?
>
>
>
> Cherie Parker
>
> Amsino Medical USA
>
> 1150 Antioch Pike, Suite 200
>
> Nashville, TN 37211
>
> Ph:615-332-9959
>
> Fax:615-831-1817
>
> cherie_parker@...
>
>
>
>

I thought contact plates went out with bleeding times. I don't think
there are any clear meaninful guidelines for how to quantify plates.
What may be an acceptable count now may be TNTC in a few hours. The
environment is rather dirty so what is the purpose? To try to educate
someone on how to clean? It might be more important to examine what
cleaning solutions they are using. Most cleaning crews I've run into
seem to make the strongest formula they can instead of going by the
manufacturers directions. How many times have you walked into a public
bathroom and been assaulted by the fumes of the disinfectant? If a
solution works best at 2% it doesn't
work better at 10 or 20%, but look
at what they are doing. Even in our lab, most of our bleach solutions
were much more than the 5% recommended. After washing a bench down, it
sometimes smelled like an over chlorinated pool!
We stopped doing any environmental cultures because no one could
interpret them.
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
yjgent@...

------------------------------

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

End of Microbio Digest, Vol 53, Issue 5
***************************************

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Rodac Plates

2009-10-06T16:49:53Z

On 2009-10-05 11:41:10 -0400, "Cherie Parker"
<cherie_parker@...> said:

I thought contact plates went out with bleeding times. I don't think
there are any clear meaninful guidelines for how to quantify plates.
What may be an acceptable count now may be TNTC in a few hours. The
environment is rather dirty so what is the purpose? To try to educate
someone on how to clean? It might be more important to examine what
cleaning solutions they are using. Most cleaning crews I've run into
seem to make the strongest formula they can instead of going by the
manufacturers directions. How many times have you walked into a public
bathroom and been assaulted by the fumes of the disinfectant? If a
solution works best at 2% it doesn't work better at 10 or 20%, but look
at what they are doing. Even in our lab, most of our bleach solutions
were much more than the 5% recommended. After washing a bench down, it
sometimes smelled like an over chlorinated pool!
We stopped doing any environmental cultures because no one could
interpret them.
--
John Gentile MS, M(ASCP)
Laboratory Information Mgr.
VA Medical Center
Providence, RI
yjgent@...

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Rodac Plates

2009-10-05T08:41:10Z

We use Rodac (contact) plates for our environmental monitoring. Is there a
method to estimate TNTC plates? How do you read spreader colonies on a
contact plate? Is there a standard method for counting colonies on a Rodac
plate?

Cherie Parker

Amsino Medical USA

1150 Antioch Pike, Suite 200

Nashville, TN 37211

Ph:615-332-9959

Fax:615-831-1817

cherie_parker@...

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

(no subject)

2009-10-02T07:58:14Z

I am using an E. coli BL21(DE3) clone with IPTG as an inducer. However though there is significant expression at the flask level, very little or no expression in 1L fed batch fermentations. Any suggestions?

_________________________________________________________________
Live Search extreme As India feels the heat of poll season, get all the info you need on the MSN News Aggregator
http://news.in.msn.com/National/indiaelections2009/aggregator/default.aspx_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Microbio Digest, Vol 53, Issue 1

2009-10-01T16:31:14Z

agree with limbic - we've enough BS melodrama around already.

-----Original Message-----
From: microbio-request@...
To: microbio@...
Sent: Thu, Oct 1, 2009 1:04 pm
Subject: Microbio Digest, Vol 53, Issue 1

Send Microbio mailing list submissions to
microbio@...

To subscribe or unsubscribe via the World Wide Web, visit
http://www.bio.net/biomail/listinfo/microbio
or, via email, send a message with subject or body 'help' to
microbio-request@...

You can reach the person managing the list at
microbio-owner@...

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Microbio digest..."

Today's Topics:

1. Re: Rising Plague (N10)

----------------------------------------------------------------------

Message: 1
Date: Thu, 1 Oct 2009 00:26:08 +0100
From: "N10" <limbic_lesion@...>
Subject: [Microbiology] Re: Rising Plague
To: microbio@...
Message-ID: <mrudnXnyTKGJdF7XnZ2dnUVZ8rCdnZ2d@...>

You would do better to go get a Doctorate in Pharmacology or Microbiology
and do something about this problem you percevive than actually prophiting
from writing about it.

N10

"sbm" <remailer@...> wrote in message
news:W9QH9LTC40076.9594328704@......

> "Rising Plague: The Global Threat from Deadly Bacteria and
> Our Dwindling Arsenal to Fight Them" by Brad Spellberg
> (Amazon.com: http://xrl.us/RisingPlague )
>
> (M&C) - Meant as a serious wakeup call to professionals and
> laypersons alike, this is a well researched yet
> approachable examination of a frightening future as the
> adaptability of bacteria threatens to overtake humanity's
> ability to create effective antibiotics. As drug companies
> continue to focus on the more lucrative markets of erectile
> dysfunction or overactive bladders, the number of new
> antibiotics under development has dwindled to a pitiful
> few. This, at a time when numerous strains of bacteria have
> developed alarming resistance to every antibiotic currently
> available and kill over 100,000 Americans annually..
>
> Continued: http://xrl.us/RisingPlague2
>
>
>

------------------------------

_______________________________________________
Microbio mailing list
Microbio@...
o.net
http://www.bio.net/biomail/listinfo/microbio

End of Microbio Digest, Vol 53, Issue 1
***************************************

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Rising Plague

2009-09-30T16:26:08Z

You would do better to go get a Doctorate in Pharmacology or Microbiology
and do something about this problem you percevive than actually prophiting
from writing about it.

N10

"sbm" <remailer@...> wrote in message
news:W9QH9LTC40076.9594328704@......

_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio

Re: Can any body give some suggetion about growing biofilm on RO membrane

2009-09-22T06:53:53Z

sajeesh k wrote:
> Respected Reserchers,
> I am trying to set a chemostat experimental set up using CDC reacter for
> growing Aeromonas hydrophila biofilm on Reverse osmosis membrane.
>
> Can any body give suggetion about the strength of LB media sutable for the
> experimnet and the dilution facter.Also how long i shuld run the chemostat
> to get a robust biofilm on RO surface.

Not to be nasty, but the entire purpose of experimentation
is to try variations and find out what works on your own...
if someone tells you what or how to do it, you're not de-
signing an experiment, you're following a protocol.

Deirdre
_______________________________________________
Microbio mailing list
Microbio@...
http://www.bio.net/biomail/listinfo/microbio