Nabble - Bio.net - Toxicol

Predictive Human Toxicity and ADME/Tox Studies 2010

2009-10-22T06:54:26Z

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Predictive ADMET Workshop: Application of Predictive ADME and Toxicology methods to drug discovery and development, Oxford July 27-31, 2009

2009-04-22T08:30:14Z

I am posting information on a 5 day workshop taking place at Oxford
University in July 2009.

27-31 July, 2009
Predictive ADMET Workshop: Application of Predictive ADME and
Toxicology methods to drug discovery and development, a Hands-on 5 Day
eCheminfo Workshop. Case Study Approach on ADME and Tox endpoints and
datasets.
http://echeminfo.com/comty_oxfordadmet09

Medical Sciences Teaching Centre,
Oxford University, Oxford, UK
Bursarys award available (deadline February 20, 2009)
facilitated by Barry Hardy
eCheminfo Community of Practice

These workshops are aimed to provide a set of stimulating workshops
using latest advanced in silico techniques of relevance to chemists,
life scientists, clinical researchers, toxicologists and modellers
working in drug discovery and development. Participants should return
to their labs with new ideas, best practices and software experiences
to maximise productivity in their own drug discovery and development
research activities.

Workshop groups will study problems with hands-on examples using
leading-edge software and discuss complex issues highlighted by
examples and case studies presented by instructors. A case study set
will be used to link all workshop activities through the week.

Workshop Instructors
Barry Hardy (Douglas Connect), Ismael Zamora (Pompeu Fabra University
and Lead Molecular Design), Christoph Helma (in silico toxicology),
David Leahy (University of Newcastle), Philip Judson (Lhasa), Anton
Hopfinger (University of New Mexico), Mark Cronin (Liverpool John
Moores University), Olga Obrezanova (BioFocus DPI), Glen Myatt
(Leadscope), Judith Madden (Liverpool John Moores University), Arianna
Bassan (S-IN)

Software packages and an IT classroom will be used by instructors and
participants to work through Predictive ADME and Toxicology problems.
Participants may propose problems and issues to the faculty ahead of
the workshop. Participants will also have ample opportunity to discuss
their perspectives and criticisms of the methods studied and should
take-away key nuggets of understanding from these intensive sessions.

A related workshop will run the week before
20-24 July, 2009
Drug Discovery Design Methods & Applications: Virtual screening,
structure-based drug design, cheminformatics and molecular modelling
supporting drug discovery and design, a Hands-on 5 Day eCheminfo
Workshop. Case Study approach including Kinases.
http://echeminfo.com/comty_oxfordworkshop09

For further information and questions on the Workshop programs, please
contact Dr. Barry Hardy at: barry.hardy -[at]- douglasconnect.com,
Tel: +41 61 851 0170
For registrations, please contact Nicki Douglas, nicki.douglas -[at]-
douglasconnect.com, Tel: +41 61 851 0461

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Alcohol death

2009-03-05T12:25:22Z

Case is a young man starting drinking at 10:00 pm and reported to have stopped drinking at 10:30. It is alleged that he did not consume any additioanl alcohol after that time. He arrived home at about midnight and died at 3:50 am with a BA of .37.

In my conclusion correct in that he would have had to drank additional alcohol between 10:30 and midnight?

Can his BA have been going down at the time of his death or does death occur at his peak of .37?

Thanks
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(no subject)

2008-12-10T02:40:38Z

Dear all,

It is known that my heavy metal ions are toxic to animals and plants. But
why are they toxic? Can your guys explain a little bit in detail about the
mechanisms that why they can cause toxicity.

Thank you very much.

Xiang Ming
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Determination of oxytocin in biological samples by isocratic high-performance liquid chromatography with coulometric detection using C18 solid-phase extraction and polyclonal antibody-based immunoaffinity column purification

2008-11-24T15:59:18Z

Determination of oxytocin in biological samples by isocratic high-performance liquid chromatography with coulometric detection using C18 solid-phase extraction and polyclonal antibody-based immunoaffinity column purification

by Mark A. Kukucka and Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University, Blacksburg 24061

ABSTRACT

A specific high-performance liquid chromatographic (HPLC) method is described for the reliable quantitation of oxytocin using culture media supernatants. The procedure employs solid-phase extraction, antibody-based immunoaffinity purification and isocratic HPLC with dual channel coulometric detection (ED). The lower limit of detection for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative simplicity, specificity and precision, the HPLC-ED of oxytocin is an accurate and attractive alternative to many existing quantitative methods.

CITATION
Kukucka, Mark A. and Hara P. Misra. 1994. Determination of oxytocin in biological samples by isocratic high-performance liquid chromatography with coulometric detection using C18 solid-phase extraction and polyclonal antibody-based immunoaffinity column purification. J Chromatogr B Biomed Appl 653(2):139-45.

HPLC determination of an oxytocin-like peptide produced by isolated guinea pig Leydig cells: stimulation by ascorbate

2008-11-24T15:57:12Z

HPLC determination of an oxytocin-like peptide produced by isolated guinea pig Leydig cells: stimulation by ascorbate

by Mark A. Kukucka and Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University, Blacksburg 24061

ABSTRACT

Highly purified populations of guinea pig Leydig cells were incubated with a maximally stimulating dose of 100 ng/mL LH for 24 h in the presence of increasing concentrations of sodium ascorbate. Sample supernatants were extracted, concentrated under vacuum, and reconstituted with acidified absolute ethanol. Samples were analyzed for oxytocin using high-performance liquid chromatography with electrochemical detection and known concentrations of an authentic oxytocin standard. Leydig cells stimulated with 0, 25, and 50 microM ascorbate produced and secreted 40.1 +/- 1.23, 77.4 +/- 13.8, 74.2 +/- 26.3 pg of an oxytocin-like peptide, respectively, per 1 x 10(6) cells. These results indicate that guinea pig Leydig cells are capable of producing an oxytocin-like peptide de novo and that low concentrations of ascorbate stimulate the production of this peptide in Leydig cells cultured in vitro.

CITATION
Kukucka, Mark A. and Hara P. Misra. 1992. HPLC determination of an oxytocin-like peptide produced by isolated guinea pig Leydig cells: stimulation by ascorbate. Arch Androl 29(2):185-90.

Isolation and culture of highly enriched populations of Leydig cells from guinea-pig (Cavia porcellus) testes

2008-11-24T15:55:13Z

Isolation and culture of highly enriched populations of Leydig cells from guinea-pig (Cavia porcellus) testes

by Mark A. Kukucka and Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University, Blacksburg 24061

ABSTRACT

Leydig cells were isolated from adult male guinea-pig testes using a multi-step procedure involving enzymatic dissociation and Percoll-gradient centrifugation. The following description is the first account of a successful isolation of adolescent guinea-pig Leydig cells. The enriched Leydig-cell preparation routinely isolated from six intact testicles yielded approximately 5.0 x 10(6) +/- 0.7 x 10(6) (+/- SEM) Leydig cells with a viability of 98.0 +/- 0.4% as determined using the trypan-blue exclusion method. The purity of the isolated cell population as assessed by 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) staining averaged 82.5 +/- 0.8%. Under light microscopy, guinea-pig Leydig cells were polyhedral in shape with a large prominent nucleus and a distinct nucleolus. The acidophilic cytoplasm contained numerous lipid-filled vesicles. Ultrastructurally, guinea-pig Leydig cells displayed an eccentrically located ovoid nucleus with dark-staining peripheral heterochromatin. Large quantities of mitochondria, smooth endoplasmic reticulum and particulate-laden lipid droplets were also evident. The steroidogenic potential of the isolated Leydig cells was verified using a maximally stimulating dose of ovine LH (100 ng ml-1) and human CG (200 mIU ml-1). Leydig cells incubated in a shaking (120 cycles min-1) water bath for 3 h at 37 degrees C in capped polypropylene microcentrifuge tubes produced 233 +/- 21 ng and 223 +/- 18 ng testosterone per 1 x 10(6) cells when maximally stimulated with oLH or hCG, respectively. The inclusion of low (1-5 microM) levels of sodium ascorbate during culture enhanced significantly Leydig-cell viability vs. control values.

CITATION
Kukucka, Mark A. and Hara P. Misra. 1994. Isolation and culture of highly enriched populations of Leydig cells from guinea-pig (Cavia porcellus) testes. Andrologia 26(4):217-24.

Elevated concentrations of ascorbate and normoxia suppress testosterone production in cultured guinea pig Leydig cells

2008-11-24T15:53:08Z

Elevated concentrations of ascorbate and normoxia suppress testosterone production in cultured guinea pig Leydig cells

by Mark A. Kukucka and Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University, Blacksburg 24061

ABSTRACT

In recent years, several metabolic roles have been proposed for vitamin C. Recent information suggests a strong causal relationship between high endogenous levels of ascorbic acid and changes in normal reproductive biology. Using highly enriched populations of guinea pig Leydig cells, we have found that elevated levels (50 to 500 microM) of ascorbate significantly (P < 0.01) depressed testosterone production in a dose-dependent manner while low levels (0 to 10 microM) were without effect. Leydig cells incubated under hypoxic (3% oxygen) culture conditions produced significantly (P < 0.01) more testosterone than similar cells cultured under normoxic (19% oxygen) conditions. The results of this study suggest that high concentrations of ascorbate and normoxic culture conditions suppress testosterone production in isolated Leydig cells. Thus, it would seem that there exists a delicate balance between normal metabolic requirements for vitamin C and excessive ascorbate levels that might alter normal gonadal reproductive events.

CITATION
Kukucka, Mark A. and Hara P. Misra. 1994. Elevated concentrations of ascorbate and normoxia suppress testosterone production in cultured guinea pig Leydig cells. Reprod Toxicol 8(4):333-9.

The antioxidant defense system of isolated guinea pig Leydig cells

2008-11-23T15:42:56Z

The antioxidant defense system of isolated guinea pig Leydig cells

by Mark A. Kukucka and Hara P. Misra
Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University, Blacksburg 24061

ABSTRACT

Utilization of highly enriched preparations of steroidogenic Leydig cells have proven invaluable for studying the direct effects of various hormones and agents on Leydig cell function in vitro. However, recent work indicates that isolated Leydig cells are often subjected to oxygen (O2) toxicity when cultured at ambient (19%) oxygen concentrations. Because intracellular antioxidants play an important role in protecting cells against oxygen toxicity, we have investigated the intracellular antioxidant defense system of isolated Leydig cells. The cellular levels of several antioxidants including catalase, glucose-6-phosphate dehydrogenase (G-6-PDH), superoxide dismutase (SOD) of the Cu/Zn & Mn variety, glutathione peroxidase, glutathione reductase and total glutathione were quantitated using enriched populations of Leydig cells isolated from adult male guinea pig testes. Compared to whole testicular homogenates, Leydig cells contained significantly (P < 0.01) less G-6-PDH, total SOD, glutathione reductase and total glutathione, but significantly (P < 0.001) more glutathione peroxidase. Compared to hepatic values previously reported in the guinea pig, Leydig cells contain nearly 400 times less catalase, about 14 times less glutathione peroxidase and almost 11 times less glutathione reductase. Since G-6-PDH and glutathione reductase are both necessary to regenerate reduced glutathione (GSH) which couples with glutathione peroxidase to breakdown hydrogen peroxide (H2O2) under normal conditions, it is plausible that the oxygen toxicity observed in isolated Leydig cells is due to the intracellular accumulation of H2O2. Using the dichlorofluorescin diacetate (DCF-DA) assay, we found that Leydig cells incubated in the presence of 19% O2 produced significantly (P<0.001) higher levels of H2O2 with time in culture compared to Leydig cells maintained at 3% O2. These results support the hypothesis that the increased susceptibility of isolated Leydig cells to oxygen toxicity may be due, in part, to decreased amounts of certain antioxidant defenses and an increased production of the reactive oxygen species H2O2.

CITATION
Kukucka, Mark A. and Hara P. Misra. 1993. The antioxidant defense system of isolated guinea pig Leydig cells. Mol Cell Biochem 126(1):1-7.

Please help me network with Toxicologist professionals - Katrina Henderson, Recruiting Project Coordinator

2008-10-16T12:52:34Z

*Miller Ag is currently networking for top talent in the ag chemical
industry, and we'd like your help! Below is a summary of our highest
priority positions – please let us know if you are interested or forward
them on to a friend or colleague who might be qualified. Also visit our
website for more details on our openings in R&D, sales and marketing,
manufacturing, regulatory professionals, and executive management in the ag
and specialty chemical industries. *

* *

*If not for you, maybe you know someone? Please tell a friend!*

* *

*"All inquiries are 100% in confidence"** *

*Senior Mammalian Toxicologist*

*· **MS with 10-15 yrs experience or Ph.D. in Toxicology or Pharmacy
with 5-10 yrs experience in the field of Mammalian toxicology with
publication record*

*· **Expert knowledge of GLP toxicology studies for regulatory
agencies*

*· **Experience in project management, effective team player, and
customer focused attitude*

* *

*Mammalian Toxicologist*

*· **M.S. with 3-10 years experience in toxicology hazard assessment,
data analysis, GLP study design and monitoring*

* *

*Senior Ecological Toxicologist*

*· **MS in ecotoxicology, ecology, or environmental science with
10-15 years relevant experience, or PhD in ecotoxicology, ecology, or
environmental science with 7-10 years experience.*

*· **Demonstrated expertise in Aquatic, Avian, or Terrestrial
ecotoxicology of agricultural products*

*· **Experience writing dossiers, overviews, and interpretive
scientific papers for registration submissions, as well as knowledge of
regulatory and GLP requirements for ecotoxicology studies***

*Ecological Toxicologist***

*· **Entry level Ph.D. or Masters Degree in Environmental Toxicology,
Environmental Sciences, or equivalent with 3-5 years experience in
conducting or monitoring ecotoxicology studies conducted under GLP in an
industrial or regulatory setting.*

*· **Advanced understanding of ecology, chemical fate and transport
processes and experience designing and conducting ecotoxicology studies to
address regulatory requirements in the U.S. and other regulatory agencies
globally. *

*· **Must be competent with all aspects of report preparation, which
includes background research, data evaluation, report writing, table
preparation and deliverable completion with intermittent review by team
leaders. *

*· **Willing to travel up to 15%. ***

*Risk Assessment Specialist***

*· **B.S. degree in Toxicology, Environmental Sciences, Agricultural
Sciences, Nursing or equivalent with 5-10 years of relevant experience,
or M.S. degree with 3-5 years experience in toxicology hazard assessment,
data analysis, GLP study design, study monitoring. *

*· **Familiar with global e-fate, modeling, human health and risk
assessment guidance. *

*· **Evaluation of scientific data, study monitoring or GLP studies,
risk assessment and knowledge of GLPs, toxicology methods, laboratory or
study audits. *

*· **Excellent technical writing skills, statistical background, and
interpersonal communication skills*

*· **Willing to travel up to 15%*

* *

*Environmental Fate Chemist*

*· **MS in chemistry, environmental science, metabolism, residue or
ecology, with minimum 5-10 years relevant experience, OR PhD in
environmental science, metabolism, residue or ecology with 3+ years
experience.*

*· **3+ yrs experience and broad scientific knowledge in
environmental fate or residue chemistry, or similar field.*

*· **Knowledge of regulatory and GLP requirements for e-fate,
metabolism, and residue chemistry studies for agricultural chemicals.*

*· **Project management skills and demonstrated ability to
effectively manage work performed at contract labs and with external
experts.*

* *

* *

Our clients pay all fees, relocation costs if needed and offer an excellent
Corporate benefits package.

If interested in any of these or other positions with Miller Ag, please send
your resume in confidence to us. If these don't fit your needs, please share
them with others who might be qualified!

See www.MillerAg.com <http://www.millerag.com/> to find more great Ag
Chemical related positions in the regulatory field, R&D, engineering and
more!

*Send resumes in confidence to: *

*Katrina Henderson at katrina@... <katrina@...> *

*615-686-8669*
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eUpdate for BCERF, September 2008

2008-09-26T10:50:22Z

Program on Breast Cancer and Environmental Risk Factors (BCERF)
The Sprecher Institute for Comparative Cancer Research
Cornell University

BCERFs Web site: http://envirocancer.cornell.edu

Providing science-based information on cancer and environmental risk factors

==========================================

WELCOME to eUpdate. This update is provided by BCERF to alert you to the latest information on cancer and environment from our program. Find out the details and more by following the links to our web site.

==========================================

What is NEW from BCERF

NEWEST EDITION OF THE RIBBON NEWSLETTER
Volume 13, Number 3, Summer 2008

Download the ENTIRE ISSUE as a PDF file at:
<http://envirocancer.cornell.edu/newsletter/pdf/v13i3.pdf>

This issue of The Ribbon features:

RESPONDING TO CANCER AND ENVIRONMENTAL CONCERNS
An interview with Dr. Elizabeth Lewis-Michl, of the Community Exposure Research Section, Bureau of Environmental and Occupational Epidemiology, Center for Environmental Health, at the New York State Department of Health, discusses her programs approach to responding to cancer and environmental concerns.
<http://envirocancer.cornell.edu/Newsletter/articles/v13Approach.cfm>

SUMMARY of the ROCHESTER SPRING REGIONAL CANCER AND ENVIRONMENT FORUM <http://envirocancer.cornell.edu/Newsletter/articles/v13Spring08.cfm>

CORNELL CHRONICLE ARTICLE ON THE BREAST CANCER RISK OF FIREFIGHTERS
This reprinted article discusses BCERF translational research and our new brochure, Alert for Women Firefighters, on chemical exposures in firefighting that may increase the risk of breast cancer. <http://envirocancer.cornell.edu/Newsletter/articles/v13Firefighters.cfm>

==========================================

SPANISH VERSION of BROCHURE FOR WOMEN FIREFIGHTERS NOW AVAILABLE

The BCERF ALERT brochure for women firefighters is now available in Spanish. This brochure emphasizes the importance of wearing protective equipment, especially Self-Contained Breathing Apparatus (SCBA), at all phases of firefighting, since there may be exposure to chemicals that may increase the risk of breast cancer during firefighting activities.

SPANISH (Español) VERSION (with references) in HTML
<http://envirocancer.cornell.edu/learning/alert/fire08_espanol.cfm>

To request copies of the printed, full-color BCERF ALERT brochure for women firefighters (color brochure currently available only in English), please contact Lyn Netter, BCERF Administrative Assistant, at 607 254-2893 or by email at lcn4@....

==========================================

FALL REGIONAL CANCER AND ENVIRONMENT FORUM ON LONG ISLAND

Our next Regional Cancer and Environment Forum will take place at Stony Brook University, on Monday, November 3, 2008, from 1:00 pm to 7:00 pm. For more information please go to:
<http://envirocancer.cornell.edu/program/meetings.cfm>

Presentation topics include:

- Contaminants emerging in our water
- Partnerships in childrens environmental health
- Genetic variation, environmental factors, and breast cancer risk
- The Estrogen Connection: Environmental estrogens in everyday products

In addition to these presentations, during the dinner program the three BCERF Estrogen Connection videos will be shown and discussed. Young women are especially encouraged to attend and participate.

The program has a strict participant limit. Please call or email Carmi Orenstein if you are interested in attending, at (607) 255-1185 or cso1@....

==========================================

BCERF strives to provide information on environmental risk factors and the risk of breast cancer and other hormonally dependent cancers. We provide information in a variety of forms, helping our readers make informed decisions for risk reduction of breast cancer and other cancers.

If you no longer wish to receive the eUpdate please notify the list manager by sending an email to Lyn Netter at lcn4@.... Type "remove from eUpdate" in the subject line. If you know of someone who might like to receive the eUpdate, ask them to email the list manager, Lyn Netter at lcn4@.... Include "add to eUpdate" in the subject line.

This issue of eUpdate was written by Suzanne M. Snedeker, BCERF Associate Director for Translational Research. Listserve manager is Lyn Netter.

BCERF
Vet Box 31
College of Veterinary Medicine
Cornell University
Ithaca, NY 14853-6401

Phone: (607) 254-2893
Fax: (607) 254-4730
Web: http://envirocancer.cornell.edu
Program email: breastcancer@...
eUpdate list manager: lcn4@...

September 2008 edition of eUpdate

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Predictive Human Toxicity and ADME/Tox Studies 2009

2008-09-24T05:12:09Z

Depiction of the antioxidant enzyme defense system

2008-09-19T11:54:00Z

Please forgive, but I lifted this abbreviated antioxidant enzyme
defense system (in ASCII form) scheme from Dr. Mark Kukucka's PhD
dissertation because I couldn't find anything else like it online!

Mechanisms by which hypoxia augments Leydig cell viability and
differentiated cell function in vitro

by Mark A. Kukucka, MS, DVM, PhD

Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University
Blacksburg, Virginia 24061-0442

found at:
http://scholar.lib.vt.edu/theses/available/etd-06062008-170416/

H2O
/\
|
| Catalase
|
oxidases SOD | Fe+2
O2 --------> O2*- --------> H2O2 ----\----> OH* --------> H2O
| Fe+3
|
| 2GSH
| / /\
| Glutathione Glutathione
| peroxidase reductase
| \/ /
| GSSG
\/
H2O

Figure 1. The Antioxidant Enzyme Defense System Scheme

Other publications by Dr. Mark A. Kukucka
http://www.nextbio.com/b/literature/literature.nb?author=MA+Kukucka

eCheminfo Community of Practice InterAction Meeting October 14-17, Philladelphia - Latest Advances in Drug Discovery Informatics

2008-09-18T05:34:00Z

I am posting details for the eCheminfo Community of Practice
InterAction Meeting in October 2008 in Bryn Mawr, Philadelphia.
Please note that abstracts are still being accepted for posters.
Latest Advances in Drug Discovery Informatics

eCheminfo Autumn Community of Practice InterAction Meeting October
14-17, 2008 Bryn Mawr College, Bryn Mawr, Philadelphia, USA

Conference Link:
http://echeminfo.com/comty_conferences

Themes: Cheminformatics, Bioinformatics, Medicinal Chemistry, Drug
Discovery Innovation, Structure-based Drug Design, Screening, Docking,
Structural Biology, Predictive Toxicology, Predictive ADME,
Chemogenomics

Program Summary
Docking & Scoring, chaired by Chaya Duraiswami (GlaxoSmithKline)
Application of MM-PBSA Free Energy Methods in Drug Discovery, chaired
by Judith Lalonde (Bryn Mawr College)
Accurate Calculation of pKas, chaired by Paul Labute (Chemical
Computing Group)
In Silico-based Chemogenomics, chaired by Fabrice Moriaud (MEDIT)
PDB Ligands: Analysing their Structure & Binding Data, chaired by Marc
Nicklaus (National Institutes of Health)
Predictive ADME, chaired by Anthony E. Klon (Pharmacopeia Drug
Discovery)
Predictive Toxicology, chaired by Artem Cherkasov (University of
British Columbia)

Pre-Conference Workshop, 13 October
Best Practices Virtual Screening Workshop chaired by Barry Hardy
(Douglas Connect)

Speakers
John Irwin (UCSF), Georgia McGaughey (Merck), Johannes H. Voigt
(Schering Plough), Lance Westerhoff (Quantum Bio), Zsolt Zsoldos
(SimBioSys), Alexey Ornufriev (Virginia Tech), David Case (Rutgers
University), Rommie Amaro (USCD), Peter Coveney (Univ. College
London), Anna Kohlmann (Ariad Pharmaceuticals), Scott Brown (Abbott),
Emil Alexov (Clemson University), Jens Erik Nielsen (University
College Dublin, Ireland), Darren Flower (Jenner Institute, UK), Maja
Mihajlovic (City
College of New York), Michael Keiser (UCSF), Brian Marsden (University
of Oxford, UK), Alex Tropsha (UNC), John Westbrook (Rutgers), Howard J
Feldman (CCG), Igor V. Filippov (NCI), Raul Cachau (ATP, SAIC-
Frederick), Vincent T. Moy (University of Miami), Paul Hawkins
(OpenEye), Yulia Borodina (NCBI), Gerhard Wolber (Inte:Ligand,
Austria), Marc Nicklaus (NCI), James P. Snyder (Emory), Anne Chaka
(NIST), Esther Kellenberger (Univ. Strasbourg, France), Renxiao Wang
(SIOC, Shanghai, PR China), Jim Dunbar (University of Michigan), Janna
Wehrle (NIGMS), Anton Hopfinger (University of New Mexico), Heidi
Einolf (Novartis), Yojiro Sakiyama (Pfizer), Olga Obrezanova (BioFocus
DPI, UK), Anthony E. Klon (Pharmacopeia), Artem Cherkasov (University
of British Columbia, Canada), Ann Richards (US EPA), Curt Breneman
(RPI)

CFP **Abstracts are still being considered for posters**
Poster Session
All InterAction Meeting registrants are eligible to present a
Conference Poster. The Poster Sessions will take place in the evenings
in Thomas Great Hall on campus, where refreshments and dinner are also
served. Poster Abstracts (300-500 words) with Title, Institution,
Authors and Contact Information should be submitted to barry.hardy -
[at]-
douglasconnect.com Abstracts will be considered based on date of
submission and quality, and will be reviewed and accepted as they are
received. To be considered for the formal program, they should be
submitted as soon as possible.

Contact:
Program: Dr. Barry Hardy, eCheminfo Community of Practice, Douglas
Connect. Tel: +41 61 851 0170. barry.hardy -[at]- douglasconnect.com

Registration Enquiries: Nicki Douglas, Douglas Connect, Baermeggenweg
14, 4314 Zeiningen, Switzerland. Tel: +41 61 851 0461. echeminfo -
[at]-
douglasconnect.com

More information at:

InnovationWell Program
http://innovationwell.net/COMTY_conferences
http://innovationwell-bm810.eventsbot.com/

eCheminfo Program
http://echeminfo.com/COMTY_conferences
http://echeminfoBM810.eventsbot.com

Rob Reedijk
eCheminfo Community of Practice

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Potential relationship between non-enzymatic antioxidants vitamin C and oxytocin

2008-09-16T13:44:15Z

This is the first report regarding quantitation of enzymatic (eg. superoxide dismutase, glutathione reductase & glucose-6-phosphate dehydrogenase) and non-enzymatic (eg. glutathione) antioxidant levels in isolated Leydig cells that I can find in the scientific literature:

Mechanisms by which hypoxia augments Leydig cell viability and differentiated cell function in vitro (PhD Dissertation, 1993)

by Mark A. Kukucka, MS, DVM, PhD

Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University
Blacksburg, Virginia 24061-0442

ABSTRACT:

The 1980s heralded the discovery and identification of extra-pituitary sources of the neurohypophysial hormone oxytocin in non-neural tissues of several animal species. The presence, location and biosynthesis of significant amounts of oxytocin in the ovarian corpus luteum was followed by the immunocytochemical demonstration of an oxytocin-like peptide in the testicular interstitial cells. Leydig cells, which comprise up to 80% of the testicular intertubular cell population, are known to synthesize testosterone in situ. Indirect evidence indicated that an oxytocin-like peptide was also present in Leydig cells. The question arose whether this peptide was synthesized de novo by Leydig cells or was taken up and stored by the cells following biosynthesis at some other intra- and/or extra-gonadal source(s). Since luteinizing hormone (LH) and ascorbate are known to augment the production of oxytocin in ovarian granulose cells, varying concentrations of these two stimulants were used to monitor the biosynthesis of oxytocin from isolated Leydig cells in culture.

Highly enriched populations of guinea pig Leydig cells were isolated using a method that employed enzymatic dissociation and Percoll gradient centrifugation. Since ambient oxygen tensions are toxic to cultured Leydig cells leading to decreased steroidogenic capacity, the antioxidant defense system of isolated Leydig cells was discerned. Decreased levels of several antioxidants including superoxide dismutase, glutathione reductase, glucose-6-phosphate dehydrogenase and total glutathione were measured. Using the dichlorofluorescin (DCF-DA) assay, it was determined that isolated Leydig cells were capable of accumulating hydrogen peroxide (H2O2). Leydig cells maintained in an atmosphere composed of 19% oxygen produced H2O2 at a faster rate than similar cells incubated at 3% oxygen.

Using a polyclonal antibody (Ab)-based immunoaffinity column, oxytocin biosynthesis was monitored in Leydig cells incubated with a mildly stimulating dose (0.1 ng/ml) of ovine LH for 24, 48 and 72 hours in the presence of increasing concentrations of sodium ascorbate (1- 500 mM) under culture conditions of hypoxia and normoxia. Following solid phase extraction and immunoaffinity purification, sample supernatants were analyzed for both testosterone and oxytocin content as measured by radioimmunoassay (RIA) and high performance liquid chromatography-electrochemical detection (HPLC-ECD) respectively. Hypoxic culture conditions and low (1-10 mM) concentrations of sodium ascorbate augmented the production of oxytocin from Leydig cells in culture. Higher (50-500 mM) levels of ascorbate and normoxic culture conditions suppressed both testosterone and oxytocin production in isolated Leydig cells. Because oxytocin synthesis was found to be cycloheximide-sensitive, we conclude that Leydig cells possess the biosynthetic machinery necessary to manufacture oxytocin. The isolated oxytocin peptide was purified by HPLC with fraction collection followed by polyclonal-Ab immunoaffinity column chromatography. Comparison of the amino acid sequence of the isolated octapeptide with authentic oxytocin provides unequivocal evidence that Leydig cells synthesize oxytocin de novo. Considering the widespread use of vitamin C as a dietary supplement, the research reported yields valuable mechanistic information on the reproductive biologic role of vitamin C in gonadal steroid and peptide hormone metabolism.

http://scholar.lib.vt.edu/theses/available/etd-06062008-170416/

Vitamin C (aka ascorbate) is another non-enzymatic antioxidant found in significant levels within the testes. As an antioxidant, ascorbate's primary role is donate electrons to neutralize reactive species of oxygen including superoxide (to H2O2) and hydroxyl free radicals (to H2O). When ascorbate acts as a scavenger (by donating an electron to a free radical), ascorbate is oxidized in the process to the ascorbate free radical and dehydro-ascorbate. The ascorbate free radical and the dehydro-ascorbate are reduced back to ascorbate either by NADH catalyzed by semidehydroascorbate reductase (and forming NAD) or reduced glutathione (GSH) catalyzed by dehydroascorbate reductase (and forming oxidized glutathione (GSSG)).

n.b. Vitamin C also works along with glutathione peroxidase (a major free radical-fighting enzyme) to revitalize vitamin E.

Interestingly, Kukucka et. al. reported finding significant levels of oxytocin (a disulfide containing octapeptide) in isolated Leydig cells. Kukucka theorized in the introduction of his PhD dissertation (back in 1993) that open chain oxytoceine (the reduced form of oxytocin) may also act as a scavenger (by donating an electron to a free radical), oxytoceine may then be oxidized back to oxytocin. As noted above, the ascorbate free radical and the dehydro-ascorbate are reduced back to ascorbate either by NADH catalyzed by semidehydroascorbate reductase (and forming NAD) or reduced glutathione (GSH) catalyzed by dehydroascorbate reductase (and forming oxidized glutathione (GSSG)).... why couldn't the ascorbate free radical and dehydro-ascorbate be reduced back to ascorbate by reduced oxytoceine (forming closed-ring oxytocin)?

Thus, the redox potential of oxytocin <---> oxytoceine may drive ascorbate <---> dehydro-ascorbate or vice versa as part of the non-enzymatic antioxidant defense system.

Other publications by Dr. Mark A. Kukucka
http://www.nextbio.com/b/literature/literature.nb?author=MA+Kukucka

The International NanoScience Community

2008-07-29T04:44:55Z

The International NanoScience Community (http://www.nanopaprika.eu/)
is a website founded on November 2007. It's similar to others
community website, but this website is totally dedicated to the
worldwide NanoScience community. You can share your stuff with the
world here: photos, videos, art, thoughts, discussions, you name it,
just make sure it's related to NanoScience.

András Paszternák is the creator and editor of The International
NanoScience Community. András is a chemistry PhD student in Budapest,
Hungary. The comunity's first target group was the "nano" researcher
from Hungary. But he had already moved towards global network. The
community currently has more than 850 members, researchers, students,
industrial partners from Europe, India, USA and 50 others countries.

With the domain name nanopaprika.eu, (paprika is favorite spice /
Hungary) the community is aiming into nanobio area. The community is
fully equip with various functions like chat, scientific forum, 26
thematic groups (SPM, Nanotoxicity, etc.), photos, videos, e-shop,
games and so on. Hence, it's definitely a good website for member to
discuss, share infomation and develop ideas

The International NanoScience Community

--
András Paszternák
creator and editor of The International NanoScience Community
English - Web: http://www.nanopaprika.eu
German - Web:
http://www.nanopaprika.de
E-mail: editor@...

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Computational Biologist / Bioinformatician Sought for Biotech Start-up

2008-07-25T05:23:11Z

Dear member,

Looking to spend time in an interesting location? SimuGen is a
toxicogenomics company originating in Cambridge (UK), now establishing some
operations in Kuala Lumpur (Malaysia) with the aim of launching the first
product from Malaysia within a year. It'll be a web-based tool using
proprietary dose-response models of gene expression phenotypes from human
cell cultures exposed to chemicals. The aim being to help pharma develop
safer drugs. This has already attracted significant attention
internationally, and the intention is to begin a collaboration with a large
pharma company in the near future.

The company is looking for a highly driven, independent bioinfomatician /
computational biologist to conduct the R&D through to product implementation
in Malaysia. This post will last 8 months and may be suited to somebody who
is technically very good but still junior in industry, looking for a
shorter-term challange. However we would also consider somebody with more
experience and ambitions of eventually leading much of the company's R&D as
a 'product architect' in the UK or internationally. This has the potential
to grow into an exciting position, with highly competitive rewards for
achieving crucial company milestones. In the first instance the core
expectations would be:

(1) Manage the quality control of all data (from benchtop to analysis).
(2) Do extensive literature and database searches for toxicicologically
relevant candidate genes.
(3) Format and manage gene expression data for analysis.
(4) Work closely with the Chief Scientific Officer (based in the UK) and
the S-plus server team to set up the web-tool for customers.
(5) Help manage the collaboration with the pharma company.

In terms of basic skills we are looking for:

(1) Gene expression analysis (microarrays, qPCR) - crucial
(2) Ability to do database and literature searches for candidate genes -
crucial
(3) R programming skills - highly valued

The intention is to start this position in September, with a competitive
salary based on experience.

Interested persons can contact Dr Wills (CSO) with informal inquiries at
quin.wills@.... A brief list of questions they might have, in
addition to a very brief CV clearly demonstrating skill sets, project
management and research potential would be most useful.We regret that we
will not respond to queries where the candidate clearly does not have the
correct background/skills.
Best regards,

Matt van Leeuwen
Operations Director
SimuGen Asia
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Gasoline vapors

2008-03-28T21:18:14Z

I have been working in a small room where 50 plus gallons of gasoline, Lacquer thinner, mineral spirits, and other solvents are stored. There is some ventilation drawn a few inches from the floor, in the corner, through a 12"x12" vent pipe. I have started to have reoccurring medical problems, mainly repertory. I would like to know what tests I should have. To determine if any real damage has been done. What toxins are in these products? what effects does prolonged exposure have on the human body? Any information would be helpful. Robert Mango Rmango@...
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Bacitracin Given Intravenously

2007-11-15T03:41:20Z

During my wife's knee replacement surgey yesterday bacitracon was
incorrectly given intravenously instead in a wound flush.
Are there any known dangers that may occur as a result of this error?

Bud

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HFC-134a in Cars - Can it make you Crash?

2007-10-19T13:15:28Z

My father was in a motor vehicle accident 2 years ago. He just purchased
a brand new Chevy truck and was leaving the dealership when he noticed
that the air conditioner was not producing cool air. Upon inspection by
the service department they found a leak in the condenser. They did not
have the parts in stock to repair so they refilled the air system told
him to come back next week and he has on his way. My father does
remember the trip home - 45 minute ride. He was in a head on collision
and there was a fatality in the other car. Witnesses have a dad's head
down and to the left. There is no question he was out but could r134a
have done this? Could r134a find its way into the truck compartment? The
dealership and technician deny recharging the system. My father is not a
lair and has very limited knowledge of air conditioning systems. Could
you offer me any advice or help? He goes to court next week to defend
his honor. His son dan.......

Dan Delarosbil
303-477 Queen St. E.
Sault Ste.Marie, On. P6A 1Z5

Office:(705) 256-5618 x246
Fax: (705) 256-1766
Cell: (705) 542-6769

The contents of this communication, including any attachment(s), are
confidential and may be privileged. If you are not the intended
recipient (or are not receiving this communication on behalf of the
intended recipient), please notify the sender immediately and delete or
destroy this communication without reading it, and without making,
forwarding, or retaining any copy or record of it or its contents. Thank
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Note: We have taken precautions against viruses, but take no
responsibility for loss or damage caused by any virus present.

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simethicone toxicity

2007-10-09T20:37:26Z

Hi, sorry to bother you but I'm looking to find some info on simethicone as
it is the active ingredient in baby 'wind drops'.

I couldn't come up with much on the web but I did find your message asking
for info and thanking someone called Jason.

Could you please share anything you know about this substance?

Thank you

Sharon Rubina

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dmso

2007-10-09T13:09:15Z

Hello,

I have reviewed the information for DMSO on www.dmso-use.com
and I was wondering if somebody has experienced this
medicine. Can we consider this as a common way of healing?

Thank you.http://dmso-use.com

Re: Pesticide residue in cotton products

2007-09-15T00:39:34Z

See: http://www.epa.gov this might help you.

TradeNET

"David Kenny" <kennyfrankfurt@...> wrote in message
news:mailman.1492.1181940599.5139.toxicol@......
Sir,Can anyone point me in the right direction...I urgently require a source
of reliable data regarding analysis of the pesticide levels in products and
byproducts of processing raw cotton through to cotton cloth or knitted
fabrics or cotton paper. Many thanks,David Kenny

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Re: Faculty Toxicology U. Texas-Austin

2007-08-23T02:47:05Z

Sherri James wrote:

> I am looking for a Toxicologist that can read a toxicology report for my attorney. I was in an auto accident and had used meth and marijuana the day before. We are need to find out what the levels mean, if they contributed to the accident.
>
> Could you please help
>
> Thank you
>
> Sherri
>
>
> ---------------------------------
> Looking for a deal? Find great prices on flights and hotels with Yahoo! FareChase.

Hi, Serri, I am a graduate at Toxicology of Drugs.
I know the effect of the marijuana, but the concentration of marijuana
contributed to the accident, is dependent of physiology of person and
habit to use the marijuana. In this last cases, the effect of
marijuana on person, is dependent of habit and frequency to abuse it.
The marijuana is detectable in urine after 4 day from administration.
The level of marijuana necessary to determinate a accident is not
important because the marijuana is illegal and any concentration is
illegal and make a risk. I know that in 1 cigarette of marijuana there
are 50 mg of active substance, but only 12 mg is absorption and make
the toxic and neurological effect.

Bye

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Faculty Toxicology U. Texas-Austin

2007-08-21T14:12:10Z

I am looking for a Toxicologist that can read a toxicology report for my attorney. I was in an auto accident and had used meth and marijuana the day before. We are need to find out what the levels mean, if they contributed to the accident.

Could you please help

Thank you

Sherri

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Dr amzad

2007-07-29T07:49:42Z

*Subject: Application for Post-doctoral/Research Associate/Scientist *

* *

Dear Sir,

Good morning. I would like to introducing myself, I am Dr. Md. Amzad
Hossain, a Bangladeshi National. My specialty is on synthetic organic
Chemistry (Flavonoids Chemistry) and Natural Products Chemistry (Isolation
and Characterization of the chemical constituents of medicinal plant or
fruits and vegetables such as flavonoids, terpenoids and caffeic acid
derivatives; i.e. polyphenolic compounds). I have already finished my 2
years Visiting Post-Doctoral Fellowship on medicinal plant in the School of
Pharmaceutical Sciences, Universiti Sains Malaysia, Malaysia. I have also
finished my doctoral degree on flavonoids chemistry. On the other hand, in
my country I am working in the Bangladesh Atomic Energy Commission as a
Principal Scientist on the same field. In addition, in my institute I am
also working on the environmental chemistry on the identification and
quantification of toxic polycyclic and polyphenolic organic compounds in
vegetable, fish and drinking water and also on natural products chemistry. I
have about 60 publications (both national and International Journals) is my
credit on the same field. Now I am very much interested to perform more
advanced research on the same field or any other related field. I have seen
your on going research area is more related to my field. For your
information, I am sending herewith my details bio-data with attached file
for your kind consideration and necessary action. In this context, I am
requesting your good selves to consider my appeal.

Hence, I pray your highness to kindly grant my appeal and oblige thereby for
which act of kindness I will be ever grateful to you.

Looking forward to hearing from you as early as possible.

Thanking you

Sincerely yours,

Dr. Md. Amzad Hossain

---------------------------------

Dr. Md. Amzad Hossain

Senior Scientific Officer

Chemistry Division

Atomic Energy Centre

G.P.O. Box 164, Ramna, Dhaka-1000

Bangladesh

e-mail: dramzadh@...

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application fr postdoc fellowship

2007-07-26T00:09:32Z

Dear Sir,
I would like to introduce myself as Ravinder Pal. I recently submitted my Ph. D. thesis and am looking for post doctoral position in well reputed lab to continue my research career on the subject. During my Ph. D. I worked on Role of garlic and carotenoids in isoniazid - rifampicin induced hepatic injury in rats. The work has got great attention and resulted in several publications of international repute; explaining the role of dietary nutrients (garlic and carotenoids) in preventing hepatotoxicity in rats.
From early interest in biological science, I had been inclined to perform research in the area of oxidative stress, antioxidants and cancer. In this regard, during my Ph.D I had been fortunate enough to get experience of molecular, biochemical and cellular approaches of antioxidants.
I believe that using my knowledge about human biology, my past eight years of experience and with your guidance I can make significant contribution to the field, since I have great expertise on most of basic techniques. So this is the right thing for me to start my postdoctoral career and learn the insights of research. I am sending an application for the post of postdoctoral research fellow, if u have position in urs lab. I am highly interested in your research work and wish to join your research group.
I am willing to work hard and contribute positively to your esteemed research group. I am sure that if given opportunity, I will leave nothing to prove my best. Please refer to my CV for your perusal.

Thank you so much for your time and am looking forward for your positive reply

---------------------------------
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Brass poisoning

2007-07-19T08:22:07Z

I am a physician who sees musicians with a wide range of disorders. I retired from the full time faculty at Univ. of Nebraska medical center in 1996. Also, I write a regular column for the "Horn Call", the Journal of the International Horn Society.

I am revisiting the question of "Brass poisoning". I did an extensive literature search and review of the clinical literature in the 1960s when I was a resident in Denver. I did not find any real evidence of "brass poisoning" as experienced by musicians using nonplated mouthpieces. Recently, I have had people ask me about Brass poisoning.

I saw your line in Google. I would like to get your review of the problem.

Thanks

Glenn

Glenn V. Dalrymple, M.D., FACR
Adj Clinical Prof of Radiology and Radiation Oncology.
Univ. of Nebraska Medical Center
Omaha, NE

gdalrymple@...
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TERA Meeting: Adult-Child Differences in Inhalation Dosimetry of Gases

2007-07-19T08:17:18Z

-----Original Message-----
From: General Toxicology Discussions on behalf of Ann Parker
Sent: Thu 7/19/2007 7:33 AM
To: TOXLIST@...
Subject: TERA Peer Consultation Meeting

TERA to Conduct Peer Consultation on a Draft Framework to Evaluate
Adult-Child Differences in Inhalation Dosimetry of Gases: Application to
Selected Systemically-Acting Volatile Organic Chemicals

Toxicology Excellence for Risk Assessment (TERA) will conduct a peer
consultation on a Draft Framework to Evaluate Adult-Child Differences in
Inhalation Dosimetry of Gases: Application to Selected
Systemically-Acting Volatile Organic Chemicals. A panel of expert
scientists will discuss and evaluate the revised draft framework and
provide direction for finalizing it.

In 2005, the same panel of experts met and held a peer consultation on
the original draft framework. The authors have revised the framework
based on the feedback received during the 2005 peer consultation. The
original and revised framework and background materials were prepared by
scientists from Toxicology Excellence for Risk Assessment (TERA) and
include ideas from several leading researchers in toxicokinetics and
physiologically-based pharmacokinetic (PBPK) modeling. The outcome of
this peer consultation will be used to further refine the process of
children's risk assessment. Funding for the peer consultation is
through a cooperative agreement between the U.S. EPA and TERA.

The peer consultation will be a web meeting, using a conference call for
the audio, and linking to a web meeting program site for the
presentation slides. The web meeting will be on Tuesday, August 21,
2007, from 9:00 a.m. to 1:00 p.m. The meeting will be open to the
public.

TERA has established a website for meeting registration at
http://www.tera.org/peer/adultchildtk/actkwelcome.htm. The revised
framework, panel membership, and additional meeting details will be
posted on the website when they become available. Interested persons
are encouraged to submit written technical comments for the panel's
consideration. A specific time during the meeting will be set to allow
any observers to ask clarifying technical questions or make comments.

TERA Contact: Ms. Patricia Nance: phone 513-542-7475, ext. 25; email
nance@...

Alternate Contact: Ms. Jacqueline Patterson: 513-521-7426; email
patterson@...

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Pesticide residue in cotton products

2007-06-15T13:13:54Z

Sir,Can anyone point me in the right direction...I urgently require a source of reliable data regarding analysis of the pesticide levels in products and
byproducts of processing raw cotton through to cotton cloth or knitted
fabrics or cotton paper. Many thanks,David Kenny
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Job Posting

2007-06-05T20:08:15Z

Science (Biotechnology) Curriculum Writers

Rebirth Biosciences, Inc., is seeking science (biotechnology)
curriculum/scientific/technical writers with five (5) or more years
experience. The persons should have experience in education curriculum
development; writing text or science books; research and writing; education
standards; British and\or American Grammar and English; writing for K12 and
college; and trained in biotechnology, biochemistry, etc.

Further, the writers maybe graduate students; teaching assistants;
postdoctoral fellows; professors; high school teachers or scientific
professionals in his/her respective fields.

Also, the potential writers should have experience in one or more of the
following areas:
Teaching (Microbiology, Biology, etc)
Curriculum Development (science)
Research (Biotechnology, bioscience, etc)
Plant Sciences
Animal Sciences
Environmental Sciences
Drugs development
Product Development
Assay
Manufacturing

Rebirth is seeking only experienced writers to create and develop
biotechnology curriculum for Kindergarten through high school.
Must be experienced in designing and writing standards-based science
curriculum, instruction, and student assessment. In addition to the above,
you should be experienced in biotechnology, chemistry, biology, molecular
biology, or physics. Must have at least a Bachelor Degree.
As a consultant, you will work from your place of convenience. Both part-time
and full-time positions are available.
Send resume, cover letter, references, curriculum writing samples, protocols,
etc to: info@...

--

Jackie Hermon
Human Resources
Rebirth BioScience, Inc
P. O. Box 22078
West Palm Beach, Florida 33416-2078
USA
Email: info@...

This message and any attachments are protected by federal and state laws.If
you are not the intended recipient do not open, rather,
return to the sender and delete from your machine.
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Wanted:Scientist / Senior Scientist - Toxicology, Cambridge MA

2007-05-23T06:39:09Z

Scientist / Senior Scientist - Toxicology

Responsibilities:

Responsible for conducting a wide variety of in vivo and in vitro
regulatory toxicology and safety pharmacology studies for anti-viral
compounds in various stages of preclinical research and development.
Serving as Study Monitor, this individual will identify CROs for studies
(single and repeat dose toxicity, DART, genotoxicity, carcinogenicity
studies, etc.); oversee protocol design and negotiate budgets; ensure
their timely conduct and delivery of reports; interpret and edit study
reports; and prepare summary reports for regulatory submissions. Will
assist in the preparation of the pharmacology/toxicology sections for
annual reports, IBs, INDs and NDAs, and will participate in generating
written responses to inquiries received from regulatory agencies.

Requirements:
PhD in Toxicology, Pharmacology or DVM, VMD. A minimum of 5-years
post-doctoral industrial experience (pharmaceutical, biotechnology,
contract research organizations) with a proven record of designing,
implementing, managing and analyzing in vivo and in vitro preclinical
toxicology and safety pharmacology studies to support small molecule
developmental candidates. Significant experience serving as Study
Monitor for studies placed at various contract research organizations
(CROs)

Working knowledge of good laboratory practice (GLP) regulations and
federal and global regulatory (FDA, ICH, OECD, etc.) guidances and
guidelines. Experience in filing INDs and other regulatory submissions,
and interfacing with regulatory authorities.

Post-doctoral training/experience in ADME/PK, immunology or infectious
diseases preferred.

Idenix offers an excellent benefits package including competitive
salary, equity compensation, major medical insurance, dental insurance,
401(k) plan participation, and group life and disability insurance.

To be considered for this position, please send your resume by email to
hr@..., or by mail to:
Human Resources
Idenix Pharmaceuticals
60 Hampshire Street
Cambridge, MA 02139

www.idenix.com

Idenix Pharmaceuticals, Inc., headquartered in Cambridge, MA, is a
biopharmaceutical company engaged in the discovery, development and
commercialization of drugs for the treatment of human viral and other
infectious diseases. Idenix's current focus is on the treatment of
infections caused by hepatitis B virus, hepatitis C virus and human
immunodeficiency virus. For more information about Idenix
Pharmaceuticals, please visit our website at www.idenix.com
<http://www.idenix.com/> .

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Academic programs in Toxicology Courses

2007-05-23T05:36:50Z

Hi Dr, Jose M Cobo

May I have list for PhD program in toxicology an/or environmental
toxicology in these countries:

Australia, Canada , UK, and US

What do you prefer for studying PhD (which universities do you prefer
and country for international student)?

Thanks

Hattan Ahmed AL-Harbi

food Sector

* Tel. No. +9661-275-9222 Ext. 217

6 Fax: +9661-275-7238

* Email: haharbi@...

* P.O.Box 84983 Riyadh 11681

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post doc position

2007-05-15T21:20:53Z

A research postdoctoral position is available in Dr. Shashi Ramaiah’s
laboratory in the Departments of Pathobiology and Interdisciplinary
Program in Toxicology to study molecular mechanisms underlying
inflammatory alcoholic liver and pancreatic disease. NIH-funded
Research involves identification of novel interacting proteins and
biochemical characterization of signal transduction pathways governing
inflammation in rodent and in vitro models.

Qualifications: A highly competent motivated PhD is sought with a solid
background in cell biology and expertise in molecular biological methods
for the study of inflammation in liver and pancreas. Experience in
molecular and cellular biology, signaling transduction, inflammation
biology, cell culture and rodent models is required. Recent graduates
preferred and proficiency in written and spoken English required. Please
submit curriculum vitae, summary of research experience, and contact
information of three references. Send all inquiries to Shashi Ramaiah,
DVM, Ph.D., Department of Pathobiology, MS 4467, College of Veterinary
Medicine, Texas A&M University, College Station, TX, email:
Sramaiah@...

**********************************************************
Shashi K. Ramaiah, DVM, PhD, DACVP, DABT
Assistant Professor
Faculties of Pathobiology and Toxicology
Texas Veterinary Medical Center
College of Veterinary Medicine
Texas A&M University
College Station, TX 77843-4467

Tel: 979-458-4725 (Off); 979-845-4672 (Lab)
Fax: 979-845-9972
sramaiah@...
**********************************************************

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Re: TCDD and Sex Ratio

2007-05-11T10:38:34Z

Thank you very much sir, I find out a lot of interesting sources..I think
that this is a really great argument..thinking also to the fact that Dioxin
is always more present in our environment.
Thank you very much!
Fabio from Italy

"Miller, Charles A III" <rellim@...> ha scritto nel messaggio
news:mailman.1047.1178802875.5139.toxicol@......
There is a growing body of literature on the subject of dioxin and related
chemicals and their effects on the ratios of male to female offspring.

Below are just a few examples.

Charles A. (Chuck) Miller III
Environmental Health Sciences Dept.
374 J.B. Johnston Bldg.
Tulane University Health Sciences Center
1430 Tulane Ave.
New Orleans, LA 70112
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1: Ishihara K, Warita K, Tanida T, Sugawara T, Kitagawa H, Hoshi N. Related
Articles
Does Paternal Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) affect
the Sex Ratio of Offspring?
J Vet Med Sci. 2007 Apr;69(4):347-52.
PMID: 17485921 [PubMed - in process]

2: Rowlands JC, Budinsky RA, Aylward LL, Faqi AS, Carney EW. Related
Articles, Links
Sex ratio of the offspring of Sprague-Dawley rats exposed to
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in utero and lactationally in a
three-generation study.
Toxicol Appl Pharmacol. 2006 Oct 1;216(1):29-33. Epub 2006 Jun 5.
PMID: 16740287 [PubMed - indexed for MEDLINE]

3: Ikeda M, Tamura M, Yamashita J, Suzuki C, Tomita T. Related Articles,
Links
Repeated in utero and lactational 2,3,7,8-tetrachlorodibenzo-p-dioxin
exposure affects male gonads in offspring, leading to sex ratio changes in
F2 progeny.
Toxicol Appl Pharmacol. 2005 Aug 15;206(3):351-5.
PMID: 16039946 [PubMed - indexed for MEDLINE]

4: Ikeda M, Mitsui T, Setani K, Tamura M, Kakeyama M, Sone H, Tohyama C,
Tomita T. Related Articles, Links
In utero and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin in
rats disrupts brain sexual differentiation.
Toxicol Appl Pharmacol. 2005 May 15;205(1):98-105.
PMID: 15885269 [PubMed - indexed for MEDLINE]

5: Pesatori AC, Consonni D, Bachetti S, Zocchetti C, Bonzini M, Baccarelli
A, Bertazzi PA. Related Articles, Links
Short- and long-term morbidity and mortality in the population exposed to
dioxin after the "Seveso accident".
Ind Health. 2003 Jul;41(3):127-38. Review.
PMID: 12916742 [PubMed - indexed for MEDLINE]

6: Ryan JJ, Amirova Z, Carrier G. Related Articles, Links
Sex ratios of children of Russian pesticide producers exposed to dioxin.
Environ Health Perspect. 2002 Nov;110(11):A699-701.
PMID: 12417498 [PubMed - indexed for MEDLINE]

7: Gale RW, Bergeron JM, Willingham EJ, Crews D. Related Articles, Links
Turtle sex determination assay: mass balance and responses to
2,3,7,8-tetrachlorodibenzo-p-dioxin and 3,3',4,4',5-pentachlorobiphenyl.
Environ Toxicol Chem. 2002 Nov;21(11):2477-82.
PMID: 12389929 [PubMed - indexed for MEDLINE]

8: James WH. Related Articles, Links
Parental exposure to dioxin and offspring sex ratios.
Environ Health Perspect. 2002 Sep;110(9):A502; discussion A502. No abstract
available.
PMID: 12204835 [PubMed - indexed for MEDLINE]

9: Schnorr TM, Lawson CC, Whelan EA, Dankovic DA, Deddens JA, Piacitelli LA,
Reefhuis J, Sweeney MH, Connally LB, Fingerhut MA. Related Articles, Links
Spontaneous abortion, sex ratio, and paternal occupational exposure to
2,3,7,8-tetrachlorodibenzo-p-dioxin.
Environ Health Perspect. 2001 Nov;109(11):1127-32.
PMID: 11712997 [PubMed - indexed for MEDLINE]

10: Moshammer H, Neuberger M. Related Articles, Links
Sex ratio in the children of the Austrian chloracne cohort.
Lancet. 2000 Oct 7;356(9237):1271-2. No abstract available.
PMID: 11072968 [PubMed - indexed for MEDLINE]

11: Trasler J. Related Articles, Links
Paternal exposures: altered sex ratios.
Teratology. 2000 Aug;62(2):77-8. No abstract available.
PMID: 10931503 [PubMed - indexed for MEDLINE]

12: Mocarelli P, Gerthoux PM, Ferrari E, Patterson DG Jr, Kieszak SM,
Brambilla P, Vincoli N, Signorini S, Tramacere P, Carreri V, Sampson EJ,
Turner WE, Needham LL. Related Articles, Links
Paternal concentrations of dioxin and sex ratio of offspring.
Lancet. 2000 May 27;355(9218):1858-63.
PMID: 10866441 [PubMed - indexed for MEDLINE]

13: Rogan WJ, Gladen BC, Guo YL, Hsu CC. Related Articles, Links
Sex ratio after exposure to dioxin-like chemicals in Taiwan.
Lancet. 1999 Jan 16;353(9148):206-7. No abstract available.
PMID: 9923880 [PubMed - indexed for MEDLINE]

14: Michalek JE, Rahe AJ, Boyle CA. Related Articles, Links
Paternal dioxin and the sex of children fathered by veterans of Operation
Ranch Hand.
Epidemiology. 1998 Jul;9(4):474-5. No abstract available.
PMID: 9647916 [PubMed - indexed for MEDLINE]

15: Vartiainen T, Jaakkola JJ, Saarikoski S, Tuomisto J. Related Articles,
Links
Birth weight and sex of children and the correlation to the body burden of
PCDDs/PCDFs and PCBs of the mother.
Environ Health Perspect. 1998 Feb;106(2):61-6.
PMID: 9432971 [PubMed - indexed for MEDLINE]

16: Mocarelli P, Brambilla P, Gerthoux PM, Patterson DG Jr, Needham LL.
Related Articles, Links
Change in sex ratio with exposure to dioxin.
Lancet. 1996 Aug 10;348(9024):409. No abstract available.
PMID: 8709758 [PubMed - indexed for MEDLINE]

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