Nabble - Bio.net - Virology

Most cited research reagents and kits

2009-12-01T16:50:02Z

An interactive graph showing most cited research reagents and kits
that are
currently available in the market. The data is based on recent
published journal articles, patents and patent applications. The
current research reagent/kits listings are: Transfection reagents, RT-
PCR, Real-time PCR, RNA purification, cAMP assays, Cytotoxicity assays
and Western blot reagents. Please visit:

http://www.sciclips.com/sciclips/most-cited-reagents.do?cat=TransfectionReagents\
&catId=1501

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Theories on why RNA viruses enter the Nucleus

2009-11-27T06:54:34Z

And no, I don't mean retroviruses. I'm talking about flaviviruses like West Nile Virus. WNV uses importin to enter the nucleus even though its an RNA virus. anyone have any ideas on why?

retrovirus

2009-10-09T06:35:41Z

Dear Sir or Madam,

I have been hearing recently that it is a retrovirus that maybe the
cause of ME/CFS.

Abstract:

In today's issue of Science Express, researchers at the Whittemore-
Peterson Institute (WPI), the Cleveland Clinic and the National Cancer
Institute report that 67% of 101 chronic fatigue syndrome (CFS)
patients tested positive for infection with xenobiotic murine
retrovirus (XMRV), a gammaretrovirus associated with a subset of
prostate cancer. Only 3.7% of 218 healthy subjects tested were
positive for the virus. Read the joint press release issued at 2:00pm
today.

Can you tell me:

Are all retrovirii contagious?

Is the XMRV xenobiotic murine retrovirus contagious?

I found this link: http://www.bio.net/bionet/mm/virology/1995-December/005003.html
which was helpful in explaining what a retrovirus is.

How is a retrovirus contracted, with emphasis on the contagious nature
of the XMRV?

Thank you very much for your help.

Yours sincerely

Marianne Gutierrez NAMMT, MICHT
www.mariannegutierrez.com

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University of Texas: It Takes $5 to Help Fund an HIV Vaccine! (no spam)

2009-10-01T08:37:05Z

Help support the research of Dr. Sudhir Paul of The University of
Texas Houston Medical School, who has published the development of a
chemically activated prototype vaccine that causes production of
protective antibodies against genetically-diverse strains of HIV in an
animal model.

Now he needs your help to continue his research so they can raise the
money to begin human trials. It’s easy, just go to endHIV.com to
donate.

A video has been created to spread the word about Dr. Paul’s work, and
we need your help.

http://www.youtube.com/watch?v=JjP_H5n33Eo

The video features several celebs (Wilson Cruz, Lady Bunny, Angie
Pontani, Thea Gill, Clifford Banagale, Kristen Renton and more) and a
prominent HIV activist who was born HIV positive (Hydeia Broadbent).

Visit this web-site and DONATE: http://covalentimmunology.org/sections/donate.html

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Lifespan of a Virus

2009-09-25T18:32:09Z

Hello,

Holy crap is this site ever old. Does anyone maintain this? Anyway
I was wondering if you ever got an answer to your Virus Lifespan question,
and if so could you show me where to look. I've alway been curious about
this one too.

Sincerely,

Graham Neil
Calgary, Canada.
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high/low pathogenicity of flu virus

2009-08-06T16:06:18Z

Dear all,

I have a very naive question... How to classify high and low
pathogenicity of influenza viruses? And where can I obtain such a
list of each category?

Thanks in advance!
Ming

==
Ming Zhang
T-6/CNLS, K710
Los Alamos National Lab
Los Alamos, NM 87544

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RE: When a Herpes Simplex Virus "dies" (M2)

2009-08-05T14:49:54Z

The viruses are not really "alive", as the only way they can replicate themselves is by hijacking the cellular machinery of the host. In order to do this, they need to be able to enter the cells. This is a complicated process and typically glycoproteins present in the envelope of enveloped viruses (such as herpesviruses) play a role in this step. So, damage to the envelope would result in the inability of the virus to enter the cell and therefore the virus would be considered "dead" as it cannot propagate itself any longer. It may still "look" like an infectious virus, e.g. has the typical herpesviral structure under electron microscope, but pretty much what's remaining is a mixture of viral proteins, lipids and sugars, which in time would "disappear" as any organic matter does...

This is why enveloped viruses are typically pretty fragile and do not survive well outside their hosts - most of them can be easily "killed" with common disinfectants. In general, they also do not survive well drying or heating (e.g. sunlight), but may retain their ability to infect new hosts for longer (e.g. days) in the dark, humid environment (e.g. a cave, a shaded water trough etc).

Magda Dunowska, LW (vet), PhD
Senior Lecturer in Veterinary Infectious Diseases (Virology)
Institute of Veterinary, Animal and Biomedical Sciences
Te Kura Mātauranga Kararehe
Massey University
Palmerston North
New Zealand

Phone : (06) 356-9099 ext 7571
Website : http://ivabs.massey.ac.nz

-----Original Message-----
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Sent: Thursday, 6 August 2009 5:04 a.m.
To: virology@...
Subject: Virology Digest, Vol 39, Issue 1

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Today's Topics:

1. When a Herpes Simplex Virus "dies" (M2)

----------------------------------------------------------------------

Message: 1
Date: Tue, 04 Aug 2009 22:54:48 -0500
From: M2 <M2slo2cht@...>
Subject: [Virology] When a Herpes Simplex Virus "dies"
To: bionet-virology@...
Message-ID: <lpvh75t7abkvumpol4pq2f1jucbo2cb25s@...>
Content-Type: text/plain; charset=us-ascii

I'm a layman with a question.
I've been told that a Herpes Simplex Virus dies almost immediately
when it sloughs off the surface of the skin. But I've also been told
that a virus isn't actually a living organism. So my question is, what
actually happens to HSV when it "dies"? I've always assumed it's
envelope disintegrates and it simply falls apart and disappears.. Am
I close?
Thanks in advance

------------------------------

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When a Herpes Simplex Virus "dies"

2009-08-04T20:54:48Z

I'm a layman with a question.
I've been told that a Herpes Simplex Virus dies almost immediately
when it sloughs off the surface of the skin. But I've also been told
that a virus isn't actually a living organism. So my question is, what
actually happens to HSV when it "dies"? I've always assumed it's
envelope disintegrates and it simply falls apart and disappears.. Am
I close?
Thanks in advance

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about PhD programme

2009-07-28T03:58:39Z

Dear sir namaste

I am microbiologist completed Masters in medical microbiology from Tribhuvan university.
Now i am working as microbiologist at National Public Health laboratory. Here i have been involved to perform RT-PCR for Pandemic influenza A/H1N1 2009, to orient Laboratory personnel about new influenza virus and HIV Viral load.We are using primers provided by CDC and follow same protocol.I have been working here from last 2 years.From last two years we are running Antimicrobial Resistant programme of selected pathogens.

I am much interested for further study in the related field especially for PhD programme.
Actually i am seeking for scholarship programme. Please kindly provide me detail information about scholarship programme for PhD at your university.

I have attached my CV along with this mail please find it.

Hope for positive consideration

Bal Ram Adhikari
Microbiologist
National Public Health Laboratory
Teku, Kathmandu

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Biocontainment Facilities Inquiry

2009-05-05T23:14:05Z

Hello,

I am working on a research paper about biocontainment facilities and
known or suspected incidents which may have affected the communities
which facilities reside in.

Do you know of (or where I may find) a comprehensive list of
biocontainment facilities?

Are you aware of any biocontainment failure resources which may be of
interest?

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Lifespan of a Virus

2009-05-01T16:39:00Z

Great question - it's one I've been curious about for many years. I was
just looking for the answer and found your question.
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proteasome/luciferase

2009-03-16T01:44:49Z

Dr Alain Kohl,

I saw your posting at www.bio.net that you made some years ago. We are
also trying to use luciferase as a proteasome reporter and we are also
getting some really weird stuff.

Did your work lead to a publication in this area? What do you think is
going on?

- Joshua Kruger, UCSD Graduate Student

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Re: Viral Load levels of HIV and HHV-6A

2009-03-03T17:23:08Z

In article <mailman.394.1236118182.13724.virology@...>,
marla rea <msrea@...> wrote:
>I am trying to reach James Scutero regarding his Lysine chart. This
>was the email address that came up. If it is correct, could he please
>respond to this email?

Scutero reportedly died in 1998.

Ian
--
Ian York (iayork@...) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England

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Viral Load levels of HIV and HHV-6A

2009-03-02T21:25:49Z

I am trying to reach James Scutero regarding his Lysine chart. This
was the email address that came up. If it is correct, could he please
respond to this email?

Thank you

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ambisense RNA

2009-02-01T10:49:02Z

How can you tell if ssRNA is ambisense, or negative sense, or positive sense in the lab?

penton and hexon number

2009-01-28T12:15:16Z

Dear Paul Clark: I am working with the possibility of interrupting the rhinovirus at one of it's developmental stages by binding it with a charged particle in vivo. However, I have not been able to find the correct hexon or penton number with the accuaracy that you have. If you don't mind me asking, which method did you use.

Tim O'Brien

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Hepatitis B virus. need some help

2009-01-07T01:44:17Z

HBV has 4 groups of proteins-polymerase. core,surface and x protein. what is the order of thier expression. I it is hard to find an article about it because of the lack of in vitro systems in the research of this virus
thanks

short courses

2008-12-10T16:25:54Z

I finsihed my PhD few years ago. I have been working with energy
metabolism and gene expression. However life and work moved me to a
new field: virology. I am starting to work with marine viruses.
Unfortunately, this is a new field and i lack the knowledge of several
things related to viruses. But I am delighted with this "life" forms.
I am very interested to attend an intensive course or a short course
of basic virology. Does anyone knows if somebody is offering such a
course? I would really appreciate this information.

Thanks in advance for your kind attention

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Re: The emergence of hepatitis C infection - please help

2008-10-25T19:18:36Z

On Mon, 20 Oct 2008 13:32:50 -0700 (PDT), biostudent
<mp00010@...> wrote:

>
>Hi people,
>
>I'm doing a piece of work at university which asks me to explain the
>"emergence of hepatitis C infection and show how human activities have
>contributed to its transmission across the centuries".
>
>Try as I might I cannot find any journals or information about the history
>of hepatitis C except its discovery in 1989, which I already know about. It
>must have been around for centuries in different countries etc...
>
>Can anyone give me any titles of appropriate journals or give me any search
>tips.

It would help if you gave an idea of what you have tried.

So let's start at the beginning. You've searched PubMed for something
like
hepatitis C history
??

The number of hits may be large, so you might choose "reviews".

Of course, you can vary that search. "transmission" might be a good
term.

You know that Hep-C used to be called non-A non-B -- before the C
virus was actually isolated?

I am surprised you can't figure out how it used to be transmitted by
reading recent info, even in textbooks, on how it is now transmitted.

"Emergence" is tricky. Look how hard it has been to figure out how HIV
emerged in humans -- and this is rather recent. You'll need to try to
trace it as best you can, learn about other hosts, and then somehow
suggest a story.

Some thoughts to start with.

bob

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The emergence of hepatitis C infection - please help

2008-10-20T13:32:49Z

Hi people,

I'm doing a piece of work at university which asks me to explain the "emergence of hepatitis C infection and show how human activities have contributed to its transmission across the centuries".

Try as I might I cannot find any journals or information about the history of hepatitis C except its discovery in 1989, which I already know about. It must have been around for centuries in different countries etc...

Can anyone give me any titles of appropriate journals or give me any search tips. Seems really hard to find.

Many thanks for any help

Cervical Swabs for HPV PCR

2008-07-30T01:11:49Z

Hello,I would like to hear from anyone who is actually doing PCR for Human
Papillomaviruses on DNA from cells obtained by cervical swabs, cytobrush,
cervical lavage etc. Basically, what works best for you? For example,
which do you recommend.. brush or swab or?,is the swab, brush etc. left dry
in collection tube?, in a transport media?, at what temperature? How best
to separate cells? extraction procedure? and so on.
Many thanx for any good info.
Dr.Sumeyra kocturk from Turkey

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virus life

2008-06-24T08:29:01Z

Hello Philipp,

Didi you get any reply on this question. I was too looking for the same.

It would be great if you reply

Thanks

Ekta
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From Paul Tucker

2008-06-02T04:29:59Z

Dear Colleague,

I'd like to remind you of the

*VIZIER / SPINE2 Workshop on “Structural Virology*

taking place 14th - 16th July, 2008, Vienna, Austria

see: http://www.projects.mfpl.ac.at/structural-virology/

We can accept up to 100 participants (on a first come first serve
basis) and although we're not there yet the number is starting to
increase.

On behalf of the Organizers,

Paul Tucker

************************************************************************
Dr. Paul A. Tucker

Group Leader

EMBL Hamburg Outstation Tel: +49 40 89902129
c/o DESY, Notkestrasse 85 FAX: +49 40 89902149
D22603 Hamburg Email tucker@...
Germany

************************************************************************

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Re: Molecular weight of influenza virus proteins

2008-05-31T20:43:49Z

On Wed, 28 May 2008 15:14:12 +0300, "Sagit Ziv-Sefer"
<ziv@...> wrote:

>Does some one know the molecular weight of Influenza virus proteins (HA,
>NP, M, NS etc.) and how do they shown on an SDS-PAGE gel?

http://www.pnas.org/cgi/content/abstract/75/8/3625

The paper should be freely available there.

bob

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Molecular weight of influenza virus proteins

2008-05-28T05:14:12Z

Does some one know the molecular weight of Influenza virus proteins (HA,
NP, M, NS etc.) and how do they shown on an SDS-PAGE gel?

Thanks

Sagit

Ziv@...

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RE: RE: particle to pfu ratio

2008-05-11T19:27:17Z

Magda,

Thanks for the correction. I was confusing genome size with virion diameter. Parvovirus is much smaller (approximately 20 nm versus 50 nm).

The low sensitivity of electron microscopy is an issue for clinical diagnosis but should not be for virus grown in culture. I do not have personal experience in quantitation or the accuracy thereof.

I will be interested in data on the accuracy of PFU measurements too. Since it involves serial dilution of a stock the accuracy would depend on how many dilution steps are used before actual plating.

Parmjeet

Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237

-----Original Message-----
From: virology-bounces@... [mailto:virology-bounces@...] On Behalf Of Dunowska, Magda
Sent: Sunday, May 11, 2008 5:56 PM
To: Randhawa, Parmjeet; virology@...; virology@...
Subject: RE: [Virology] RE: particle to pfu ratio

Hi everybody,
With respect to Parmjeet's email below: I got the 5.2 from a description in a veterinary virology book, but after your email I did check that info. So, parvovirus genomes are 5 kbp long according to the latest report on virus taxonomy (http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.050.1.01.htm) and the available full genome sequences of porcine parvovirus on GenBank are about this length (a link to a recent submission of a whole sequence of 5075 bp: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9628826).

Also, I am not sure how accurate the EM would be for quantifying viral particles, as it is a pretty insensitive tool and one needs at least 10*6 particles/mL to be even able to see them under EM, but I would be very interested to know whether or not anybody out there have used it for this purpose?

Magda Dunowska, LW (vet), PhD
Senior Lecturer in Veterinary Infectious Diseases (Virology)
Institute of Veterinary, Animal and Biomedical Sciences
Te Kura Mātauranga Kararehe
Massey University
Palmerston North
New Zealand

Phone : (06) 356-9099 ext 7571
Website : http://ivabs.massey.ac.nz

-----Original Message-----
From: Randhawa, Parmjeet [mailto:randhawapa@...]
Sent: Saturday, 10 May 2008 5:43 p.m.
To: Dunowska, Magda; virology@...; virology@...
Subject: RE: [Virology] RE: particle to pfu ratio

I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus
particles could also be quantitated by electron microscopy but that will
not distinguish viable from non-viable virions.

Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237

-----Original Message-----
From: virology-bounces@...
[mailto:virology-bounces@...] On Behalf Of Dunowska,
Magda
Sent: Friday, May 09, 2008 7:14 PM
To: virology@...; virology@...
Subject: [Virology] RE: particle to pfu ratio

Doria,
If you have a pure virus prep, maybe you could titrate it (to get PFU/mL
value), and then extract viral DNA and estimate the number of viral
particles based on the amount of viral DNA present, knowing that
parvoviruses have a single-stranded DNA genome about 5.2 kbp in size?
Just a thought...

Magda

----------------------------------------------------------------------

Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers@...>
Subject: [Virology] particle to pfu ratios
To: <virology@...>
Message-ID:

<783CE756031AB141B53257D89039DD75075EFFA3@...>
Content-Type: text/plain; charset="US-ASCII"

We are wondering what the particle:PFU ratio is for porcine parvovirus.

Any ideas how to acquire this statistic?

Doria Bowers

------------------------------

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***************************************

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RE: RE: particle to pfu ratio

2008-05-11T14:55:49Z

Hi everybody,
With respect to Parmjeet's email below: I got the 5.2 from a description in a veterinary virology book, but after your email I did check that info. So, parvovirus genomes are 5 kbp long according to the latest report on virus taxonomy (http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.050.1.01.htm) and the available full genome sequences of porcine parvovirus on GenBank are about this length (a link to a recent submission of a whole sequence of 5075 bp: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9628826).

Also, I am not sure how accurate the EM would be for quantifying viral particles, as it is a pretty insensitive tool and one needs at least 10*6 particles/mL to be even able to see them under EM, but I would be very interested to know whether or not anybody out there have used it for this purpose?

Magda Dunowska, LW (vet), PhD
Senior Lecturer in Veterinary Infectious Diseases (Virology)
Institute of Veterinary, Animal and Biomedical Sciences
Te Kura Mātauranga Kararehe
Massey University
Palmerston North
New Zealand

Phone : (06) 356-9099 ext 7571
Website : http://ivabs.massey.ac.nz

-----Original Message-----
From: Randhawa, Parmjeet [mailto:randhawapa@...]
Sent: Saturday, 10 May 2008 5:43 p.m.
To: Dunowska, Magda; virology@...; virology@...
Subject: RE: [Virology] RE: particle to pfu ratio

I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus
particles could also be quantitated by electron microscopy but that will
not distinguish viable from non-viable virions.

Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237

-----Original Message-----
From: virology-bounces@...
[mailto:virology-bounces@...] On Behalf Of Dunowska,
Magda
Sent: Friday, May 09, 2008 7:14 PM
To: virology@...; virology@...
Subject: [Virology] RE: particle to pfu ratio

Doria,
If you have a pure virus prep, maybe you could titrate it (to get PFU/mL
value), and then extract viral DNA and estimate the number of viral
particles based on the amount of viral DNA present, knowing that
parvoviruses have a single-stranded DNA genome about 5.2 kbp in size?
Just a thought...

Magda

----------------------------------------------------------------------

Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers@...>
Subject: [Virology] particle to pfu ratios
To: <virology@...>
Message-ID:

<783CE756031AB141B53257D89039DD75075EFFA3@...>
Content-Type: text/plain; charset="US-ASCII"

We are wondering what the particle:PFU ratio is for porcine parvovirus.

Any ideas how to acquire this statistic?

Doria Bowers

------------------------------

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Could this be a possible treatment for HIV/AIDS?

2008-05-10T15:08:56Z

Could this be a possible treatment for HIV/AIDS:

Synthesize a peptide nucleic acid antisense oligonucleotide for HIV’s
gag gene’s mRNA. The reason for using PNA is that it is substantially
more resistant to enzyme degradation by nucleases and proteases. The
reason for choosing the gag gene is that it’s proteins, p24, p17, p7,
and p6, code for the basic physical infrastructure of HIV; w/o these
key proteins, there is no HIV. Then, encapsulate the oligonucleotides
in liposomes studded w/ anti-CD4 antibodies. This will ensure 1)
toxicity is limited—cf Ambisome, the liposomal preperation of
amphotericin B—2) the biologic goes only where it’s needed, which is
the cytoplasm of CD4+ T-cells. Decorating the liposome w/ anti-CD4
antibodies will trigger endocytosis, in my limited knowledge, at
least. I predict low toxicity and high efficacy in reducing the
patient’s viral load to hopefully 0 copies/ml.

Maybe, when I'm doing my PhD or MD dissertation --a long ways off,
see
below--, I could do it on this, patent the resulting product --ie the
PNA oligonucleotide itself--, and license it to Genentech or Hybridon
for further development into a marketable biologic.

What are your thoughts? Should LNA instead of PNA be used? Cell-
penetrating peptides vs. liposomes? Targeting the gag gene vs.
targeting the pol gene? Using anti-CD4 mAbs vs. anti-gp160 mTcRs as a
drug targeting device? Are there any publications about this subject?

Another drug could be a mTcR for gp160, linked to FasL...this should
cause formation of DISC, leading to the cell's eventual apoptosis. And
since it's linked to an mTcR that only recognizes gp160, only HIV-
infected cells will undergo apoptosis. And since apoptosis is an
organized "format c:" of the cell, the virons will also be
destroyed!!!

In your expert opinions, do you believe these will cause remission of
HIV/AIDS? Partial or complete?

And, just for fun, what do you think the generic names would be for
these biologics? Brand names?

BTW, if this helps you answer, I'm a 16.5 year old community college
freshman who hopes to have a career as a physician-scientist.

Thanks!!!!

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RE: RE: particle to pfu ratio

2008-05-09T22:43:16Z

I think parvovirus is smaller than 5.2 kb, more like 2-3 kb. Virus
particles could also be quantitated by electron microscopy but that will
not distinguish viable from non-viable virions.

Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237

-----Original Message-----
From: virology-bounces@...
[mailto:virology-bounces@...] On Behalf Of Dunowska,
Magda
Sent: Friday, May 09, 2008 7:14 PM
To: virology@...; virology@...
Subject: [Virology] RE: particle to pfu ratio

Doria,
If you have a pure virus prep, maybe you could titrate it (to get PFU/mL
value), and then extract viral DNA and estimate the number of viral
particles based on the amount of viral DNA present, knowing that
parvoviruses have a single-stranded DNA genome about 5.2 kbp in size?
Just a thought...

Magda

----------------------------------------------------------------------

Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers@...>
Subject: [Virology] particle to pfu ratios
To: <virology@...>
Message-ID:

<783CE756031AB141B53257D89039DD75075EFFA3@...>
Content-Type: text/plain; charset="US-ASCII"

We are wondering what the particle:PFU ratio is for porcine parvovirus.

Any ideas how to acquire this statistic?

Doria Bowers

------------------------------

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End of Virology Digest, Vol 29, Issue 1
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RE: particle to pfu ratio

2008-05-09T16:14:16Z

RE: Virology Digest, Vol 29, Issue 1

2008-05-09T15:49:55Z

Doria,
If you have a pure virus prep, maybe you could titrate it (to get PFU/mL value), and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought...

Magda

-----Original Message-----
From: virology-bounces@... [mailto:virology-bounces@...] On Behalf Of virology-request@...
Sent: Thursday, 8 May 2008 5:04 a.m.
To: virology@...
Subject: Virology Digest, Vol 29, Issue 1

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----------------------------------------------------------------------

Message: 1
Date: Wed, 7 May 2008 12:26:10 -0400
From: "Bowers, Doria" <dbowers@...>
Subject: [Virology] particle to pfu ratios
To: <virology@...>
Message-ID:
<783CE756031AB141B53257D89039DD75075EFFA3@...>
Content-Type: text/plain; charset="US-ASCII"

We are wondering what the particle:PFU ratio is for porcine parvovirus.

Any ideas how to acquire this statistic?

Doria Bowers

------------------------------

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End of Virology Digest, Vol 29, Issue 1
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particle to pfu ratios

2008-05-07T09:26:10Z

We are wondering what the particle:PFU ratio is for porcine parvovirus.

Any ideas how to acquire this statistic?

Doria Bowers

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VIZIER / SPINE2 Workshop on Structural Virology

2008-04-15T14:56:15Z

Dear Virologist,

Registrations are now being accepted for the

*VIZIER / SPINE2 Workshop on Structural Virology*

taking place from 14th - 16th July 2008, in Vienna, Austria

This workshop will cover recent advances in our understanding of viral
entry, assembly, replication and pathogenesis principally, but not
exclusively, based upon information derived from structural studies. The
planned sessions are on viral assembly, double-stranded RNA viruses, the
flavivirus replication machinery, the coronavirus replication machinery,
virus/host interactions including aspects of viral entry, negative
strand RNA viruses and viruses of prokaryotes and archea.

In addition to the invited speakers, oral presentations will be selected
from the submitted abstracts. The sessions are not exclusive and any
topic relevant to the structural biology of viruses will be considered
for a poster or oral presentation. For this reason early registration
and abstract submission is strongly encouraged.

The workshop is part of the training and dissemination activities of the
Vizier and Spine2Complexes projects. The Department of Biomolecular
Structural Chemistry, Max F. Perutz Labs at University of Vienna is a
Training and Dissemination Centre for the EU 6th Framework VIZIER
project (http://www.vizier-europe.org <http://www.vizier-europe.org/>).

*
INVITED SPEAKERS INCLUDE*:
*
Virus assembly*:
Alasdair Steven (NIAMS)

*Double-stranded RNA viruses*:
Jon Grimes (Oxford), B.V.V. Prasad (Baylor College of Medicine)

*Flavivirus replication machinery*:
Martino Bolognesi (Milano), Bruno Canard (Marseilles), Hongmin Li (NY
State Dept. of Health)

*Coronavirus replication machinery*:
Rolf Hilgenfeld (Lubeck)

*Viral entry/Virus host interactions*:
Felix Rey (Institute Pasteur), Katsumi Maenaka (Fukuoka), Winfried
Weissenhorn (Grenoble)

*Negative strand RNA viruses*:
Rob Ruigrok (IVMS Grenoble), Stephen Graham (Oxford), Zihe Rao
(Tsinghua)

*Viruses infecting bacteria and archea*:
Herman van Tilbeurgh (Orsay), Denis Bamford (Helsinki)

The number of participants is limited to 100, the applicants will be
accepted on a first-come first-served basis.

More information on this Workshop (all details, description,
registration) are available at:
http://www.projects.mfpl.ac.at/structural-virology/

******Deadline for abstract submission and registration is 15th June
2008.

We strongly encourage you to register soon, even if you do not have all
registration details in hand, and BOOK a hotel ASAP! Vienna is a busy
city in summer.

You can complete/confirm your registration afterwards, until the
registration deadline June 15th, 2008, using the link that will be
sent to you via
e-mail.

Please feel free to forward this email to anyone you think might be
interested.

Paul Tucker

************************************************************************
Dr. Paul A. Tucker

Group Leader

EMBL Hamburg Outstation Tel: +49 40 89902129
c/o DESY, Notkestrasse 85 FAX: +49 40 89902149
D22603 Hamburg Email tucker@...
Germany

************************************************************************

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International Science and Engineering Fair - Call for Judges

2008-03-31T11:09:52Z

The 59th ISEF will be held this year in Atlanta, Georgia May
11th-17th. I am looking for volunteers to serve as Grand Awards
Judges in the Microbiology Category. Last year's ISEF in Albuquerque,
New Mexico, over 45 different countries sent 1510 secondary school
finalists to compete. We expect the same turn out at this year's
Fair. I am looking for judges to choose the top finalists who will
compete for thousands of dollars in prizes and awards. Grand Awards
Judges will arrive in Atlanta on Tuesday, May 13 for orientation and
pre-judging and will judge on Wednesday, May 14. This is the premier
worldwide science research competition for high school students grades
9-12 and we need your support. If you are interested in serving as a
judge at this year's ISEF, please go to the following website and
register:

http://www.intelisef2008.org/judging.html

If you need more information, don't hesitate to contact me at
drkjroberts@...

Karl J. Roberts, Ph.D.
Co-Chair, Microbiology Division
International Science and Engineering Fair

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TCID50

2008-03-25T05:10:24Z

Hi,

Any recommendations. I am doing so viral replication assays under
hypoxia and normoxia. This is causing my cells to grow a different
rates through the time points.

I am trying to nomalise the data. Should i normalise to cells at time
of infection as standard. At 24 hours, for cell count at each time
point or use a ration of cell growth under different conditions. I
have counted cell number under different conditions at all time
points.

Stuggling to find any detail in the literature to support this.

Many Thanks

Crispin

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