Blue high MW electrophoresis tracking dye?

[Raymond Dalgleish-2]

Last week, a laboratory practical that I teach was ruined because our
Central Teaching Unit provided blue loading dye that clearly did not
migrate on 1% agarose with the 300 bp mobility expected of bromophenol
blue. The dye was perhaps a little darker than BPB and appeared to have
a mobility around 10 kb. The consequence was that all of the PCR
products ran off the bottom of the gel.

Any ideas about what the blue dye might have been in this incorrect
loading dye?

Many thanks,

Raymond Dalgleish

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[Christian Praetorius]

Raymond Dalgleish <rwmd1@...> wrote:

>Any ideas about what the blue dye might have been in this incorrect
>loading dye?

This sounds very much like Xylene cyanol. Our loading biuffer contains
both dyes.

Christian

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X-no-Sig: yes
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