hey there,
thanks a lot for your quick replies and possible solutions! I am
probably going to try the Catterall dissociation technique, but as I
am under some time pressure it would be better if I could solve my
problem with Ca2+ current recording in the acute hippocampal slices.
Has anyone experienced that in slices, Ca2+ currents are insensitive
to channel blockers, e.g. conotoxin? Currents show an initial rundown
but after that, a stable baseline normally persists. I have ruled out
an application error, and Na+ and K+ currents are sufficiently
blocked. Series resistance doesn't increase a lot during the
experiments.
Again, I'd be delighted if someone had a possible explanation!
Sibylle
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