Continuous method for the large-scale extraction of plasmid DNA by modified boiling lysis (Nature)

[Aseemmello]

Uni-BT (BIB)
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-4GGWG44-3&_user=616141&_coverDate=08%2F22%2F2005&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000032320&_version=1&_urlVersion=0&_userid=616141&md5=8054ef27b5f0c8ea142fc5a0fda66f4d#SECX19

Als PDF:
 
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3C-4GGWG44-3-F&_cdi=4943&_user=616141&_orig=browse&_coverDate=08%2F22%2F2005&_sk=998819996&view=c&wchp=dGLbVzW-zSkWz&md5=c2197cdff173187bd2a9bf921ef2b523&ie=/sdarticle.pdf

Nature
http://www.nature.com/nprot/journal/v1/n6/abs/nprot.2006.452.html

lysis at 70°C -> extraction

10l/h => ca. 100 mg plasmid DNA

Stichwort: DNA