If you know the size in base pairs of the virus, you can calculate the
molecular weight of the viral DNA (1 nucleotide PAIR= 660 daltons).
Viral DNA concentration (calculated from OD 260 value) can then be
converted to viral particles using Avogadro's number (1 Gram MW=
6.02e+23 viral particles).
Parmjeet Randhawa, M.D.
Professor of Pathology,
Division of Transplant Pathology,
University of Pittsburgh, Department of Pathology,
E737 UPMC-Montefiore Hospital,
3459 Fifth Ave, Pittsburgh, PA 15213.
Phone: 412 647 7646
Fax: 412 647 5237
-----Original Message-----
From:
virology-bounces@...
[mailto:
virology-bounces@...] On Behalf Of bob
Sent: Saturday, March 22, 2008 12:18 PM
To:
bionet-virology@...
Subject: [Virology] Re: Determination of particle/pfu ratio
On Mon, 17 Mar 2008 14:21:36 -0700 (PDT), ukats <
ukats02@...>
wrote:
>Dear all,
>
>I have OD 260 value and PFU/ml value of purified virus
>sample. How then do I calculate particle/pfu ratio
>using these values?
>
So, do you know how many virus particles corresponds to 1 OD unit?
If not, you will need to measure it. Electron microscopy? Or some
labeling.
bob
_______________________________________________
Virology mailing list
Virology@...
http://www.bio.net/biomail/listinfo/virology_______________________________________________
Virology mailing list
Virology@...
http://www.bio.net/biomail/listinfo/virology
