Exonerate and GFF3 for GBrowse??

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Exonerate and GFF3 for GBrowse??

by Bob Freeman-2 :: Rate this Message:

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Does anyone know how to go from Exonerate output to GFF3? Currently, it only outputs GFF2.

I thought I read that importing GFF2 into Chado is not a good idea, esp. when trying to represent ESTs and mRNAs (cDNAs) aligned against scaffolds. So, I'm hunting around for a way to convert my Exonerate EST/mRNAs alignments to GFF3 for importing into Chado.

Or am I going about this bass-ackwards?

TIA,
Bob

-----------------------------------------------------
Bob Freeman, Ph.D.
Acorn Worm Informatics, Kirschner lab
Dept of Systems Biology, Alpert 524
Harvard Medical School
200 Longwood Avenue
Boston, MA  02115
617/432.2293, vox

The only other person I've apologized to is my mother and that was court ordered.
-- Karen Walker from Will & Grace




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Parent Message unknown Re: Exonerate and GFF3 for GBrowse??

by Don Gilbert-6 :: Rate this Message:

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Bob,

Jason Stajich wrote a useful process_exonerate_gff3 perl; I've a variant of that
I use, here:
http://eugenes.org/gmod/genogrid/scripts/process_exonerate_gff3.perl
which avoids use of bioperl and is somewhat faster.

- Don Gilbert
-- d.gilbert--bioinformatics--indiana-u--bloomington-in-47405
-- gilbertd@...--http://marmot.bio.indiana.edu/

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Re: Exonerate and GFF3 for GBrowse??

by Bob Freeman-2 :: Rate this Message:

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Thanks, Don. You're a life saver. All the links pointing to Jason's  
old script are dead. Either that, or I haven't search far enough.

-b

On Nov 9, 2009, at 5:38 PM, Don Gilbert wrote:

>
> Bob,
>
> Jason Stajich wrote a useful process_exonerate_gff3 perl; I've a  
> variant of that
> I use, here:
> http://eugenes.org/gmod/genogrid/scripts/process_exonerate_gff3.perl
> which avoids use of bioperl and is somewhat faster.
>
> - Don Gilbert
> -- d.gilbert--bioinformatics--indiana-u--bloomington-in-47405
> -- gilbertd@...--http://marmot.bio.indiana.edu/

-----------------------------------------------------
Bob Freeman, Ph.D.
Acorn Worm Informatics, Kirschner lab
Dept of Systems Biology, Alpert 524
Harvard Medical School
200 Longwood Avenue
Boston, MA  02115
617/432.2293, vox

The only other person I've apologized to is my mother and that was  
court ordered.
        -- Karen Walker from Will & Grace




------------------------------------------------------------------------------
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trial. Simplify your report design, integration and deployment - and focus on
what you do best, core application coding. Discover what's new with
Crystal Reports now.  http://p.sf.net/sfu/bobj-july
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Re: Exonerate and GFF3 for GBrowse??

by Jason Stajich-3 :: Rate this Message:

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sorry bob - I am guessing don's will work fine. some things are  
defunct when my former inst stopped supporting some of my websites.

trying to migrate important things to git when I am reminded they are  
misising - this folder has some variants of that script in there  
including a + and - bioperl one.
  http://github.com/hyphaltip/genome-scripts/tree/master/data_format/

-jason
On Nov 9, 2009, at 3:45 PM, Bob Freeman wrote:

> Thanks, Don. You're a life saver. All the links pointing to Jason's
> old script are dead. Either that, or I haven't search far enough.
>
> -b
>
> On Nov 9, 2009, at 5:38 PM, Don Gilbert wrote:
>
>>
>> Bob,
>>
>> Jason Stajich wrote a useful process_exonerate_gff3 perl; I've a
>> variant of that
>> I use, here:
>> http://eugenes.org/gmod/genogrid/scripts/process_exonerate_gff3.perl
>> which avoids use of bioperl and is somewhat faster.
>>
>> - Don Gilbert
>> -- d.gilbert--bioinformatics--indiana-u--bloomington-in-47405
>> -- gilbertd@...--http://marmot.bio.indiana.edu/
>
> -----------------------------------------------------
> Bob Freeman, Ph.D.
> Acorn Worm Informatics, Kirschner lab
> Dept of Systems Biology, Alpert 524
> Harvard Medical School
> 200 Longwood Avenue
> Boston, MA  02115
> 617/432.2293, vox
>
> The only other person I've apologized to is my mother and that was
> court ordered.
> -- Karen Walker from Will & Grace
>
>
>
>
> ------------------------------------------------------------------------------
> Let Crystal Reports handle the reporting - Free Crystal Reports 2008  
> 30-Day
> trial. Simplify your report design, integration and deployment - and  
> focus on
> what you do best, core application coding. Discover what's new with
> Crystal Reports now.  http://p.sf.net/sfu/bobj-july
> _______________________________________________
> Gmod-gbrowse mailing list
> Gmod-gbrowse@...
> https://lists.sourceforge.net/lists/listinfo/gmod-gbrowse

--
Jason Stajich
jason.stajich@...
jason@...


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Re: Exonerate and GFF3 for GBrowse??

by Bob Freeman-2 :: Rate this Message:

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Thanks, Jason. I didn't want to bug you until I needed it ... getting the Exonerate data in has been on the back burner for some time.

In any case, I'll download this as well.

Congrats, btw, on the faculty job!

Best,
Bob

On Nov 11, 2009, at 2:43 AM, Jason Stajich wrote:

sorry bob - I am guessing don's will work fine. some things are
defunct when my former inst stopped supporting some of my websites.

trying to migrate important things to git when I am reminded they are
misising - this folder has some variants of that script in there
including a + and - bioperl one.
http://github.com/hyphaltip/genome-scripts/tree/master/data_format/

-jason
On Nov 9, 2009, at 3:45 PM, Bob Freeman wrote:

Thanks, Don. You're a life saver. All the links pointing to Jason's
old script are dead. Either that, or I haven't search far enough.

-b

On Nov 9, 2009, at 5:38 PM, Don Gilbert wrote:


Bob,

Jason Stajich wrote a useful process_exonerate_gff3 perl; I've a
variant of that
I use, here:
http://eugenes.org/gmod/genogrid/scripts/process_exonerate_gff3.perl
which avoids use of bioperl and is somewhat faster.

- Don Gilbert
-- d.gilbert--bioinformatics--indiana-u--bloomington-in-47405
-- gilbertd@...--http://marmot.bio.indiana.edu/


-----------------------------------------------------
Bob Freeman, Ph.D.
Acorn Worm Informatics, Kirschner lab
Dept of Systems Biology, Alpert 524
Harvard Medical School
200 Longwood Avenue
Boston, MA  02115
617/432.2293, vox

The only other person I've apologized to is my mother and that was court ordered.
-- Karen Walker from Will & Grace




------------------------------------------------------------------------------
Let Crystal Reports handle the reporting - Free Crystal Reports 2008 30-Day
trial. Simplify your report design, integration and deployment - and focus on
what you do best, core application coding. Discover what's new with
Crystal Reports now.  http://p.sf.net/sfu/bobj-july
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