We are developing a reverse genetics strategy for finding mutations in
specific genes. Presently we are using uv mutagenesis but would like to
try other mutagens to get a broader spectrum of mutants. We have tried a
frameshift mutagen, ICR-191. Does anyone have any experience to maximize
the mutation rate using ICR-191? Is there other frameshift mutagens we
could try with chlamy?
Thanks,
Marilyn
--
<>-<>-<>-<>-<>-<>-<>-<>-<>-
Marilyn Kobayashi
Niyogi Lab
441 Koshland Hall
Plant and Microbial Biology
University of California
Berkeley, CA 94720
510-643-6604
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