Lectins

[hippie dream]

Hello All,

I recently purchased some TRITC conjugated Lectin from Triticum vulgaris from Sigma (L5266-2MG). I intend to use this lectin to label EPS components in a river biofilm. The lectin is shipped as a lyophilized powder. I following the instruction of Neu & Lawrence (Methods in Microbiology, 2004). They suggest preparing a stock solution by adding 1mL of buffer for every 1mg of lectin.

My issue is that they suggest using either filter sterilized water or a buffer. My quandary is that I don't know which to use. I don't even really know how to assess which one to use and if I am using a buffer other than water which do I use? If anyone has any ideas that would be fantastic.

Thanks!

Sam

[Nick Theodorakis-2]

On Jun 23, 2:43 pm, hippie dream <tonightstheni...@...> wrote:

> Hello All,
>
> I recently purchased some TRITC conjugated Lectin from Triticum vulgaris
> from Sigma (L5266-2MG). I intend to use this lectin to label EPS components
> in a river biofilm. The lectin is shipped as a  lyophilized powder. I
> following the instruction of Neu & Lawrence (Methods in Microbiology, 2004).
> They suggest preparing a stock solution by adding 1mL of buffer for every
> 1mg of lectin.
>
> My issue is that they suggest using either filter sterilized water or a
> buffer. My quandary is that I don't know which to use. I don't even really
> know how to assess which one to use and if I am using a buffer other than
> water which do I use? If anyone has any ideas that would be fantastic.
>

Check the product sheet that came with the sample. If it was
lyophilized from buffer, then all you need to add is water because
buffer should be reconstituted along with the lectin.

Otherwise, choose conditions that are most compatibile with the
solubility of the lectin and won't interfere with downstream
applications.

Nick

--
Nick Theodorakis
nick_theodorakis@...
contact form:
http://theodorakis.net/contact.html
_______________________________________________
Methods mailing list
Methods@...
http://www.bio.net/biomail/listinfo/methods

[hippie dream]

On Jun 23, 2:43 pm, hippie dream <[e-mail]> wrote:
> Hello All,
>
> I recently purchased some TRITC conjugated Lectin from Triticum vulgaris
> from Sigma (L5266-2MG). I intend to use this lectin to label EPS components
> in a river biofilm. The lectin is shipped as a  lyophilized powder. I
> following the instruction of Neu & Lawrence (Methods in Microbiology, 2004).
> They suggest preparing a stock solution by adding 1mL of buffer for every
> 1mg of lectin.
>
> My issue is that they suggest using either filter sterilized water or a
> buffer. My quandary is that I don't know which to use. I don't even really
> know how to assess which one to use and if I am using a buffer other than
> water which do I use? If anyone has any ideas that would be fantastic.
>

>Check the product sheet that came with the sample. If it was
lyophilized from buffer, then all you need to add is water because
buffer should be reconstituted along with the lectin.


Thanks. Yeah it was definitely lyophilized from the buffer so all I had to do was add water. Sorry for the simple question. Thanks alot for the help.

Sam

[Dr Engelbert Buxbaum]

Am 24.06.2009, 00:29 Uhr, schrieb hippie dream  
<tonightsthenight@...>:


>> Check the product sheet that came with the sample. If it was
> lyophilized from buffer, then all you need to add is water because
> buffer should be reconstituted along with the lectin.
>
>
> Thanks. Yeah it was definitely lyophilized from the buffer so all I had  
> to
> do was add water. Sorry for the simple question. Thanks alot for the  
> help.

If you plan longer-term storage of the stock solution and the product  
specifications do not state otherwise, consider adding an equal amount of  
glycerol to the solution and store at -20 celsius. Because of the glycerol  
the solution will not freeze, avoiding damage by freeze-thaw cycles.
_______________________________________________
Methods mailing list
Methods@...
http://www.bio.net/biomail/listinfo/methods

[hippie dream]

>
> Check the product sheet that came with the sample. If it was
>>>
>> lyophilized from buffer, then all you need to add is water because
>> buffer should be reconstituted along with the lectin.
>>
>>
>> Thanks. Yeah it was definitely lyophilized from the buffer so all I had to
>> do was add water. Sorry for the simple question. Thanks alot for the help.
>>
>
> If you plan longer-term storage of the stock solution and the product
> specifications do not state otherwise, consider adding an equal amount of
> glycerol to the solution and store at -20 celsius. Because of the glycerol
> the solution will not freeze, avoiding damage by freeze-thaw cycles.
>
>

Thanks for the suggestion. I think that storing the stock solution at -20
will be an issue like you said but since I am going to be using the lectin
for only a couple months I think it will be fine to just store it at 4
degrees.
_______________________________________________
Methods mailing list
Methods@...
http://www.bio.net/biomail/listinfo/methods