Hello All,
We would like to use a luciferase expression vector to tag cells in
zebrafish embryos and were wondering if anyone has tried to use
vectors commercially available from Promega (pRL-CMV) or New England
BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the
luciferase coding region need to be inserted into a Xenopus or
Zebrafish specific vector for expression of the luciferase in the
embryo?
Kristine Griffett
University of South Florida
Department of Cell Biology, Microbiology and Molecular Biology
4202 E Fowler Ave
BSF 218
Tampa, FL 33620
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