MEG vs. PEG

[Bari Zahedi]

Has anyone used PEG to reduce melting temperature of their primers for PCR? We are working with very GC rich DNA and have been using Betane but we have read that MEG works better. We don't have any MEG but we do have PEG and were wondering if it could be substituted for MEG. Thanks in advance.

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[David-Paul Minde]

how about DMSO ? if available, I think the additive of choice (because about
everybody uses it and therefore tons of experience reports on it around).
cheers,
David

2009/10/27 Bari Zahedi <bzahedi@...>

> Has anyone used PEG to reduce melting temperature of their primers for PCR?
> We are working with very GC rich DNA and have been using Betane but we have
> read that MEG works better. We don't have any MEG but we do have PEG and
> were wondering if it could be substituted for MEG. Thanks in advance.
>
> _______________________________________________
> Methods mailing list
> Methods@...
> http://www.bio.net/biomail/listinfo/methods
>

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