Mitochondrial isolation for mt DNA extraction from Earthworms

[luiscunhamx]

Dear collegues
I wonder if anyone ever tried to isolate mitochondrias from earthworms for posterior mtDNA extraction, could anyone sen me a good protocol? I am having a lot of trouble doing that, probably due to soil contamination in the digestive tract, even after depuration and I am getting very low yield of mtDNA. Any help?? I am working with Pontoscolex corethrurus, my model organism :).

Best regards

--
Luís Cunha
(Msc, Phd student)
Centro de Investigação em Recursos Naturais, Departamento de Biologia – Universidade dos Açores, Rua da Mãe de Deus, 13A – Apartado 1422, 9501-855 Ponta Delgada
Açores, Portugal
Tel: [phone] (ext. 1476)
Fax: [phone]
Tlm ([phone]
E-mail: luisnevescunha@...
http://www.pherg.org

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