NGS data

Hi,

I'm trying out GBrowse2 to visualise some ChIPseq short read data. Our
collaborators want to be able to look at the individual short reads
(which after filtering is about 13mill ChIP reads and 11mill Input
reads). According to a previous thread
(http://www.nabble.com/NGS-in-GGB-td22477878.html): "with GBrowse2,
incorporating nextgen data should be fairly easy." Can anyone elaborate
on this? Should I be using wig files? If so, can I divvy up the original
file into small chunks and make separate binary wig and gff files for
each one? Will a MySQL database loaded with bp_seqfeature_load.pl cope
with 13 million features  or will it grind to a halt?

Thanks!
Cass

--
Dr. Caroline Johnston

0207 848 5306

James Black Centre
Centre for the Cellular Basis of Behaviour
Department of Neuroscience
Institute of Psychiatry
Kings College London
125 Coldharbour Lane
London SE5 9NU

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