PAGE gel sticked on both plates

[Nicosnake]

HI everyone, i've been having this problem whit my acrylamide gels: after acrylamide is polymerized between the two glass plates it's really hard to separate both plates and after a long effort i can take them appart only to realize that the gel has sticked to BOTH plates, the gel is actually cut in half. I treat surfaces of both plates, one with bind silane (so the acrylamide sticks to this one) and the other with Sigmacote to prevent the gel from sticking. But still the gel sticks to both surfaces and after i separate the glases it's useless. Has anyonehad this kind of trouble?? ANy suggestion is accepted.

[jianhao cao]

how about trying to soak the gel and plates in buffer for seperation

2008/12/8 Nicosnake <chamosilencio@...>:

>
> HI everyone, i've been having this problem whit my acrylamide gels: after
> acrylamide is polymerized between the two glass plates it's really hard to
> separate both plates and after a long effort i can take them appart only to
> realize that the gel has sticked to BOTH plates, the gel is actually cut in
> half. I treat surfaces of both plates, one with bind silane (so the
> acrylamide sticks to this one) and the other with Sigmacote to prevent the
> gel from sticking. But still the gel sticks to both surfaces and after i
> separate the glases it's useless. Has anyonehad this kind of trouble?? ANy
> suggestion is accepted.
> --
> View this message in context: http://www.nabble.com/PAGE-gel-sticked-on-both-plates-tp20888761p20888761.html
> Sent from the Bio.net - Methods mailing list archive at Nabble.com.
>
> _______________________________________________
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> Methods@...
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[Patricia C Arbour-Reily]

1) I have had other brands of "Sigmacote" go bad......2)Rain-X , for
your car windows , works OK but you must treat more often...its a bit
more green  and will do in a pinch while you replace Sigmacote , if gone
bad. 3) We have had plates get gunk on them from something in the wash
up...one time, a student ran all  plates thru a dishwasher and used the
correct soap for a dishwasher but the wrong soap for the plates ,
obviously...... I don't know how that was corrected
Pat


-----Original Message-----
From: methods-bounces@...
[mailto:methods-bounces@...] On Behalf Of Nicosnake
Sent: Sunday, December 07, 2008 8:44 PM
To: methods@...
Subject: PAGE gel sticked on both plates


HI everyone, i've been having this problem whit my acrylamide gels:
after
acrylamide is polymerized between the two glass plates it's really hard
to
separate both plates and after a long effort i can take them appart only
to
realize that the gel has sticked to BOTH plates, the gel is actually cut
in
half. I treat surfaces of both plates, one with bind silane (so the
acrylamide sticks to this one) and the other with Sigmacote to prevent
the
gel from sticking. But still the gel sticks to both surfaces and after i
separate the glases it's useless. Has anyonehad this kind of trouble??
ANy
suggestion is accepted.
--
View this message in context:
http://www.nabble.com/PAGE-gel-sticked-on-both-plates-tp20888761p2088876
1.html
Sent from the Bio.net - Methods mailing list archive at Nabble.com.

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[Mahesh Reddy]

Hi
When u use plates first time..u need to wash the plates with NaOH.Wash the plates with 100% Etoh before making the gel.After making gel..store in Fridge until you run the gel.you can store the gel 20 days in fridge. If u still getting problem with plates..u have to check ur buffer s. I never get any problem with my plates.

Best wishes
Mahesh

Nicosnake wrote:

HI everyone, i've been having this problem whit my acrylamide gels: after acrylamide is polymerized between the two glass plates it's really hard to separate both plates and after a long effort i can take them appart only to realize that the gel has sticked to BOTH plates, the gel is actually cut in half. I treat surfaces of both plates, one with bind silane (so the acrylamide sticks to this one) and the other with Sigmacote to prevent the gel from sticking. But still the gel sticks to both surfaces and after i separate the glases it's useless. Has anyonehad this kind of trouble?? ANy suggestion is accepted.

[Nicosnake]

Hello everybody, thanks for all your advice and recommendations. Fortunately, the sticking problem seems to be solved. After posting the original message, i tried treating both plates with Sigmacote, to see if the bind silane was the problem. I expected the gel separate from both plates but surprisingly it stayed sticked to the one that had been previously treated with bind silane. So i guess, as one of you said it might have been too much of bind silane. I'll folow the recommendation of coating just one plate with sigmacote and leaving the other one "clean" so the gel sticks to this one.
Thanks for all your help, i really appreaciate it.

[allisonh]

Nicosnake wrote:
> HI everyone, i've been having this problem whit my acrylamide gels: after
> acrylamide is polymerized between the two glass plates it's really hard to
> separate both plates and after a long effort i can take them appart only to
> realize that the gel has sticked to BOTH plates, the gel is actually cut in
> half. I treat surfaces of both plates, one with bind silane (so the
> acrylamide sticks to this one) and the other with Sigmacote to prevent the
> gel from sticking. But still the gel sticks to both surfaces and after i
> separate the glases it's useless. Has anyonehad this kind of trouble?? ANy
> suggestion is accepted.

I clean my plates with Windex every time just before using them - works
fine to prevent sticking!
Allison
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