PCR Lab Design

[Aqua Gem]

Dear Mr. Carson,

 

Please allow me to introduce myself. My name is Andrea Budd and I am working
for the Belize Agricultural Health Authority as the Coordinator of the
Aquatic Health Management Unit. A major component of this management unit is
to set up and have functional diagnostic labs for disease monitoring and by
extension - farm certification.

 

I have been searching the net for any information I could find on planning
and designing a PCR lab and it was through this means I came across your
message regarding the same topic (at www.bio.net/). Though it seems this
message is a bit dated I am hoping to make contact with you and ask for some
insight on PCR Lab design.

 

Grateful if you could respond.

 

Sincerely,

 

Andrea Budd.

 

--

Andrea Budd (Mrs.)

Aquatic Health Unit

Animal Health Department

Belize Agricultural Health Authority

 

Tel: [phone]-4457

Fax: [phone]

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[Vince Mulholland]

Dear Andrea,

In case you didn't get a reply I have attached some links to relevant publications on PCR lab design (see below). I designed a PCR lab a few years ago, and the main constraints on your design will be the space available plus any budgetary limits. The important thing in the design of PCR work spaces is compartmentalization; at least 2 rooms are required, but if you are going to be doing large-scale routine PCR you'd probably need to set the lower limit at 3 rooms.

Regards,
Vince

Links:
www.hpa-standardmethods.org.uk/documents/qsop/pdf/qsop38.pdf
www.biosupplynet.com/pdf/01_PCR_Primer_p.5_14.pdf
genome.cshlp.org/content/3/2/S2.full.pdf


Vincent Mulholland
Molecular Biology Unit Manager -  Diagnostics & Molecular Biology Section
Science and Advice for Scottish Agriculture (SASA)

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