Dear Dr. Gegenheimer,
I hope this e-mail finds you well.
I sought that you gave some reasons to explain why palindromic recognition sequences.
I found that you answer is very reasonable and indeed, because the risk or having this flanks the bacteria avoid those sequences. However, what about non-palindromic restriction sites? Those are avoided too, but are those as important as palindromic sequences? The non-symmetric restriction sites will be target of a single-stranded cleavage or same as palindrome, the enzymes can cut both DNA strands?
I will really appreciate your input.
Ana Luisa
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Ana Luisa Maldonado-Contreras
PhD Candidate
University of Puerto Rico
Rio Piedras Campus
Microbial Ecology Lab
Phone: 787 764 0000 ext. 4883
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