Sir/Madam,
I, Anthony
Palathingal, am currently doing Masters in Biotechnology at the University of
Essex, Uk. I have reached the final semester of my course which is undertaking a
project titled “Characterisation of the plant enzyme isoprene synthase and
studying its behaviour in bacterial cells”.
I would like to
enhance my knowledge in the field of Plant sciences by pursuing a PhD in the
same. I have always been interested in the genetic manifestations of the plant
& microbial kingdoms. Understanding their metabolic pathways in their
survival has always intrigued my ever inquisitive nature.
In the Masters
dissertation, I have been working on the enzyme isoprene synthase by
characterizing it in the bacterial specie E.coli. I have been able to
confirm the conserved residues in the Kudzu Isoprene synthase according to
Sharkey (2005) and have carried out site directed mutagenesis to bring about
point mutations. The study further involves checking if there lies any
difference between the amount of isoprene production between the wild type and
the mutant constructs.
The project has
helped me in understanding and practising various biological techniques, like
cloning, PCR, transformation, automated sequencing, electrophoresis, gas
chromatography, mass spectrometry.
My personal
strengths have always been about understanding the subject thoroughly before I
get down to the practical work. I am hard-working and can easily adapt to the
given situation, irrespective of the level of hardness. I would be highly
obliged if you could let me know of available PhD studentships.
Looking forward to
your positive response,
Thanking
you,
Anthony
Palathingal
Note: Please find
attached resume