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PhiX174 resistance E. coli strain?

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	PhiX174 resistance E. coli strain? by Yoram Gerchman :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Greetings all I am looking for PhiX174 resistance E. coli strain. Does anyone know of such strain? Many thanks Yoram ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	Re: PhiX174 resistance E. coli strain? by Duncan Clark :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Historians believe that in newspost <mailman.62.1255029029.1133.methods@...> on Thu, 8 Oct 2009, Yoram Gerchman <gerchman@...> penned the following literary masterpiece: >I am looking for PhiX174 resistance E. coli strain. Does anyone know of such >strain? The original hosts for phiX174 production were E.coli C derivatives. No idea if it goes into E.coli B or K12 strains. If it's genome has an EcoB or EcoK site in the sequence then that would answer the question. I think a Pubmed search is the best way to go. Duncan -- I love deadlines. I especially like the whooshing noise they make as they go flying by. Duncan Clark GeneSys Ltd. _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	Re: Re: PhiX174 resistance E. coli strain? by Yoram Gerchman :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Thanks Dunken. Could you kindly elaborate on the EcoB/EcoK issue? Since the lysis system (E protein) seem to work in K12/coli B whay would not the phage propagate in them? Many thanks Yoram > Date: Mon, 12 Oct 2009 15:24:57 +0100 > From: Duncan Clark <blackhole@...> > Subject: Re: PhiX174 resistance E. coli strain? > > Historians believe that in newspost > <mailman.62.1255029029.1133.methods@...> on Thu, 8 Oct 2009, > Yoram Gerchman <gerchman@...> penned the following > literary masterpiece: > >I am looking for PhiX174 resistance E. coli strain. Does anyone know of such > >strain? > > The original hosts for phiX174 production were E.coli C derivatives. No > idea if it goes into E.coli B or K12 strains. If it's genome has an EcoB > or EcoK site in the sequence then that would answer the question. > > I think a Pubmed search is the best way to go. > > Duncan > -- > I love deadlines. I especially like the whooshing noise they make as > they go flying by. > > Duncan Clark > GeneSys Ltd. ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	Re: PhiX174 resistance E. coli strain? by Duncan Clark :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Historians believe that in newspost <mailman.88.1255374269.1133.methods@...> on Mon, 12 Oct 2009, Yoram Gerchman <gerchman@...> penned the following literary masterpiece: >So EcoB and EcoK are >Type I restriction enzymes and will degrade the phiX174 genome Only if the genome contains a recognition site for those enzymes. If it doesn't I can't see offhand (bar any particular gene requirement) why it shouldn't infect a B or K12 strain. I wonder if the very first papers on phiX174 show strain information with regards E.coli C, K12 and B. Quite often that was one thing that was published, especially for say T-odd or T-even phages. Bit more digging required I think :-) Duncan -- I love deadlines. I especially like the whooshing noise they make as they go flying by. Duncan Clark GeneSys Ltd. _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods