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Problem with cultivation on anaerobic solid media

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	Problem with cultivation on anaerobic solid media by Luzan Tatiana :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message In my lab we use hungate bottles only for the liquid anaerobic media and bi- layer solid media. We have to do inoculations on solid meida only using Petri dishes in the anaerobic jar supplemented with gas-packs or controlled atmosphere anaerobic chamber. THere is a problem in the chamber: the cag mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While incubating plates inside, the media dries up very quickly and an enourmous condensation is forming! IT is almost a lake of water there. IS there any product which would absorb an acess condensate? Thanks a lot -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 _______________________________________________ Microbio mailing list Microbio@... http://www.bio.net/biomail/listinfo/microbio
	Re: Problem with cultivation on anaerobic solid media by N10 :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message "Luzan Tatiana" <txl8326@...> wrote in message news:mailman.615.1195074105.23109.microbio@...... > In my lab we use hungate bottles only for the liquid anaerobic media and > bi- > layer solid media. We have to do inoculations on solid meida only using > Petri dishes in the anaerobic jar supplemented with gas-packs or > controlled > atmosphere anaerobic chamber. THere is a problem in the chamber: the cag > mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While > incubating plates inside, the media dries up very quickly and an enourmous > condensation is forming! IT is almost a lake of water there. > > IS there any product which would absorb an acess condensate? > Thanks a lot > > -- > > Tanya Luzan > Research Assistant > Department of Biology > University of Louisiana at Lafayette > Phone (Office): 482-5056 > Hi Luzan How long are you incubating for ? I use a similar system and have no problems for up to 48 hours at 37'c You could try a Petri dish containg a layer of Silica gel ( dried) to absorb the humidity. Obvioulsy check for zero effects on colony yield :) Best Des _______________________________________________ Microbio mailing list Microbio@... http://www.bio.net/biomail/listinfo/microbio
	Re: Problem with cultivation on anaerobic solid media by Mad Twins Dad :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Luzan Tatiana wrote: In my lab we use hungate bottles only for the liquid anaerobic media and bi- layer solid media. We have to do inoculations on solid meida only using Petri dishes in the anaerobic jar supplemented with gas-packs or controlled atmosphere anaerobic chamber. THere is a problem in the chamber: the cag mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While incubating plates inside, the media dries up very quickly and an enourmous condensation is forming! IT is almost a lake of water there. IS there any product which would absorb an acess condensate? Thanks a lot -- Tanya Luzan Research Assistant Department of Biology University of Louisiana at Lafayette Phone (Office): 482-5056 _______________________________________________ Microbio mailing list Microbio@net.bio.net http://www.bio.net/biomail/listinfo/microbio Tanya, I would agree with using sterile selica, another option you have - just dry your plate for a while. I guess it will be rather enough to leave 1.5% agar plates overnight. Cheers. Sasha. PS I could not reach your email box, sorry.
	Re: Problem with cultivation on anaerobic solid media by N10 :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Hi A silica gel envelope helps N10 "Mad Twins Dad" <sandro_photo@...> wrote in message news:mailman.1195.1206380447.2451.microbio@...... > > > > Luzan Tatiana wrote: >> >> In my lab we use hungate bottles only for the liquid anaerobic media and >> bi- >> layer solid media. We have to do inoculations on solid meida only using >> Petri dishes in the anaerobic jar supplemented with gas-packs or >> controlled >> atmosphere anaerobic chamber. THere is a problem in the chamber: the cag >> mixture we use is 10% Hydrogen, 15% CO2 and Nitrogen as the rest. While >> incubating plates inside, the media dries up very quickly and an >> enourmous >> condensation is forming! IT is almost a lake of water there. >> >> IS there any product which would absorb an acess condensate? >> Thanks a lot >> >> -- >> >> Tanya Luzan >> Research Assistant >> Department of Biology >> University of Louisiana at Lafayette >> Phone (Office): 482-5056 >> >> _______________________________________________ >> Microbio mailing list >> Microbio@... >> http://www.bio.net/biomail/listinfo/microbio >> >> > > Tanya, I would agree with using sterile selica, > another option you have - just dry your plate for a while. > I guess it will be rather enough to leave 1.5% agar plates overnight. > > Cheers. > > Sasha. > > PS I could not reach your email box, sorry. > -- > View this message in context: > http://www.nabble.com/Problem-with-cultivation-on-anaerobic-solid-media-tp13756475p16246239.html > Sent from the Bio.net - Microbio mailing list archive at Nabble.com. > _______________________________________________ Microbio mailing list Microbio@... http://www.bio.net/biomail/listinfo/microbio