Protein Concentration Question

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Protein Concentration Question

by Mina M Jalali :: Rate this Message:

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Hey Erik,
I saw your question posted on bio.net and was wondering if you ever figured out the answer. I had a similiar question: What is the concentration of a protein in a cell in molarity?  I know that proteins account for 15% of a cell's overall concentration and that the molar mass of a protein is 50,000 g/mol.  If you could help me figure out the answer, that would be great.

Thank you!
Mina






Hi netters.  Can anyone be of help by telling me where/how I might be able
to find/calculate a "typical" concentration (mg/ml or something of the
like) of a housekeeping protein like tubulin in a "typical" mammalian
cell.  I need a ball park figure.  As far as I know, about 16-20% of a
cell's weight is protein (but what's the typical weight of a "typical"
mammalian cell?), volume is readily estimated from cell diameter, and a
highly expressed protein could be about 1% of the total protein in a cell
(so for, say tubulin, about 0.1-0.2% of the cell is protein by weight
(?)).  Any suggestions, info???? Please email.

Cheers,

erik

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