Querry regarding silver staining for glycoproteins

[Mr.Pradeep Kumar Iyer Group Leader, Bio Tech, HRF]

Dear All,

           I have this confusion on the silver staining of glycoproteins in
IEF gels. I have observed that with increasing glycosylation, the intensity
of the glycoproteins after silver stain decreases. I attribute this to the
masking of the aminoacids by the glycan chain resulting in the decrease in
efficiency of the silver stain. Has anyone done an IEF for deglycosylated
molecule? Does it give a more intense band than the glycosylated one? My
next experiment would be that but if that is observed by most of  you, I
would not waste my resources on doing that. Kindly give some inputs.

Regards,
Pradeep Iyer

--
If you're not part of the solution, you're part of the precipitate – Henry
J. Tillman
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[D.Lefeber-2]

Dear all,

Does someone know an interested researcher for a post-doc on glycan mass
spectrometry, as atteched?

Kind regards,

Dirk


Please note address change!
-----------------------------------------------------------------
Dr. D.J. Lefeber, PhD
Clinical Biochemical Geneticist
Glycosylation & Lysosomal disorders
Department of Laboratory Medicine
830 - Laboratory of Genetic Endocrine and Metabolic Diseases
Radboud University Nijmegen Medical Centre
Geert Grooteplein 10
6525 GA Nijmegen
The Netherlands
D.Lefeber@...
phone: [phone] / 3953
fax:   [phone]
http://www.umcn.nl/lkn





-----Oorspronkelijk bericht-----
Van: glycosci-bounces@...
[mailto:glycosci-bounces@...] Namens Mr.Pradeep Kumar
Iyer Group Leader, Bio Tech, HRF
Verzonden: dinsdag 20 oktober 2009 6:26
Aan: glycosci@...; methods@...
Onderwerp: [Glycosci] Querry regarding silver staining for glycoproteins

Dear All,

           I have this confusion on the silver staining of glycoproteins
in
IEF gels. I have observed that with increasing glycosylation, the
intensity
of the glycoproteins after silver stain decreases. I attribute this to
the
masking of the aminoacids by the glycan chain resulting in the decrease
in
efficiency of the silver stain. Has anyone done an IEF for
deglycosylated
molecule? Does it give a more intense band than the glycosylated one? My
next experiment would be that but if that is observed by most of  you, I
would not waste my resources on doing that. Kindly give some inputs.

Regards,
Pradeep Iyer

--
If you're not part of the solution, you're part of the precipitate -
Henry
J. Tillman
_______________________________________________
Glycosci mailing list
Glycosci@...
http://www.bio.net/biomail/listinfo/glycosci




Het UMC St Radboud staat geregistreerd bij de Kamer van Koophandel in het handelsregister onder nummer 41055629.
The Radboud University Nijmegen Medical Centre is listed in the Commercial Register of the Chamber of Commerce under file number 41055629.


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