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Re: Different rendering for wiggle tracks

by Stephen Taylor-5 :: Rate this Message:

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Hi Lincoln/Jason,

>
> The wiggle code was recently moved out of GBrowse and into a separate
> Bio::Graphics distribution. At the same time we removed the default
> smoothing to better render the raw data. Could you try to download the
> CPAN version of Bio::Graphics and install it on *top* of the GBrowse-CVS
> version? If the data continues to look wrong, I will fix it.
>

We tried it with Bio::Graphics 1.94 and no difference...at least when I just reloaded the page...

Jason, the code you pointed out

    my $trim      = $self->current_track->{display_options}{trim} || 'stdev2';

seems to fly in the face of the docs
       
http://gmod.org/wiki/Gbrowse/Uploading_Wiggle_Tracks#Wiggle_Track_Options_Supported_by_GBrowse

  trim              stdev|stdev2|stdevn          # default is none

So would it be better to change it to

    my $trim      = $self->current_track->{display_options}{trim} || 'none';

?

What I am not 100% clear on is do we have regenerate all the wig binaries to see an effect or just reload the page after these fixes (assuming mod_perl is off!)?

Thanks,

Steve



> Lincoln
>
> On Thu, May 28, 2009 at 11:06 AM, Steve Taylor
> <stephen.taylor@... <mailto:stephen.taylor@...>> wrote:
>
>     Hi,
>
>     We have two installations of gbrowse for our production and test
>     environment.
>
>     (1) The gbrowse production server was from the 1.69 tar ball using
>     ActivePerl-5.8.8.822, and run with bioperl-live as on 2008-09-04
>     (2) On the gbrowse development server we installed GBrowse-CVS as on
>     2009-03-19 using netinstall to see if this would fix some problems
>
>     However, on (2) we noticed there is a difference in the way wiggle
>     tracks are rendering. The (2) version looks smoothed and wrong
>     compared with the raw data.
>     Was this type of rendering desired? We notice the wiggle_xyplot.pm
>     <http://wiggle_xyplot.pm> code is completely different.
>
>     To see what I mean have a look at:
>
>     (1) http://sara.molbiol.ox.ac.uk/userweb/staylor/gbrowse/gbrowse.jpg
>     (2) http://sara.molbiol.ox.ac.uk/userweb/staylor/gbrowse/gbrowsedev.jpg
>
>     The age old question :-) do we wait for 1.7 to fix this or is this
>     type of rendering likely to stay?
>
>     Kind regards and thanks,
>
>     Steve
>     ------------------------------------------------------------------
>     Medical Sciences Division
>     Weatherall Institute of Molecular Medicine/Sir William Dunn School
>     Oxford University
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