|

»

Bio.net - Methods

Re: Loading dye

View: New views

6 Messages — Rating Filter: Alert me

	Re: Loading dye by Barbara MacGregor :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Xylene cyanol definitely has a different color, though, at least as I've used it - blue-green rather than blue. But those are the only blue dyes I can think of, so that's probably it... Barbara > > Raymond Dalgleish <rwmd1@...> wrote: > >> Any ideas about what the blue dye might have been in this incorrect >> loading dye? > > This sounds very much like Xylene cyanol. Our loading biuffer contains > both dyes. > > Christian > _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	RE: Loading dye by Jayakumar, R :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Bromo phenol blue.. Frequently added with xylene cyanol in DNA loading dyes. Jay -----Original Message----- From: methods-bounces@... [mailto:methods-bounces@...] On Behalf Of Barbara MacGregor Sent: Monday, October 26, 2009 1:13 PM To: methods@... Subject: Re: Loading dye Xylene cyanol definitely has a different color, though, at least as I've used it - blue-green rather than blue. But those are the only blue dyes I can think of, so that's probably it... Barbara > > Raymond Dalgleish <rwmd1@...> wrote: > >> Any ideas about what the blue dye might have been in this incorrect >> loading dye? > > This sounds very much like Xylene cyanol. Our loading biuffer contains > both dyes. > > Christian > _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	Re: Loading dye by Christian Praetorius :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Barbara MacGregor <bmacgreg@...> wrote: >Xylene cyanol definitely has a different color, though, at least as >I've used it - blue-green rather than blue. Thats right. But it migrates around 10k in a 1% gel. Christian -- X-no-Sig: yes _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	RE: Loading dye by Jayakumar, R :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message Hmm.. XC I think moves with the 4K band and above the blue dye front of BPB (the blue dye he is talking about) moves with a 300 bp band on a 1% gel Jay -----Original Message----- From: methods-bounces@... [mailto:methods-bounces@...] On Behalf Of Christian Praetorius Sent: Tuesday, October 27, 2009 5:30 AM To: methods@... Subject: Re: Loading dye Barbara MacGregor <bmacgreg@...> wrote: >Xylene cyanol definitely has a different color, though, at least as >I've used it - blue-green rather than blue. Thats right. But it migrates around 10k in a 1% gel. Christian -- X-no-Sig: yes _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	Re: Loading dye by Raymond Dalgleish-2 :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message The issue is now solved. We ran a 1% agarose gel of the suspect loading dye alongside size markers and other loading dyes of known provenance. The dye which had been described as being bromophenol blue was in fact very highly concentrated xylene cyanol, so it looked dark blue rather than blue/green. The person responsible will be re-educated. Thanks for all the replies. Raymond Jayakumar, R wrote: > > Hmm.. XC I think moves with the 4K band and above the blue dye front of BPB (the blue dye he is talking about) moves with a 300 bp band on a 1% gel > Jay > > > -----Original Message----- > From: methods-bounces@... [mailto:methods-bounces@...] On Behalf Of Christian Praetorius > Sent: Tuesday, October 27, 2009 5:30 AM > To: methods@... > Subject: Re: Loading dye > > Barbara MacGregor <bmacgreg@...> wrote: > >> Xylene cyanol definitely has a different color, though, at least as >> I've used it - blue-green rather than blue. > > Thats right. But it migrates around 10k in a 1% gel. > > Christian > > -- > X-no-Sig: yes > _______________________________________________ > Methods mailing list > Methods@... > http://www.bio.net/biomail/listinfo/methods > > > This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods
	Re: Loading dye by WS-4 :: Rate this Message: Reply to Author \| View Threaded \| Show Only this Message > The person responsible will be re-educated. Means hopefully: Will be sentenced to bake Black-Forest-Cake (under surveillance) for the next lab meeting? Cheers, Wo _______________________________________________ Methods mailing list Methods@... http://www.bio.net/biomail/listinfo/methods