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Re: Luciferase Expression Vectors
Kristine, I carried out promoter-reporter experiments in zebrafish embryos and luciferase expression vectors were active as is. I tried pRL-CMV, as well as pRL-TK and pRL-SV40. All of these give high luciferase activity at 2 dpf (or even before), when injected into the single cell.
Best wishes, Joep Brinkmann 2009/8/1 <zbrafish-request@...>
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Today's Topics:
1. RE: Zfish Digest, Vol 50, Issue 6 : How old are these fish?
(Gale Clark)
2. RE: Z Digest, Vol 50, Issue 7 fish with kinky bodies (Gale Clark)
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Message: 1
Date: Fri, 31 Jul 2009 13:02:46 -0400
From: "Gale Clark" <gclark@...>
Subject: [Zbrafish] RE: Zfish Digest, Vol 50, Issue 6 : How old are
these fish?
To: <zbrafish@...>, <zbrafish@...>
Message-ID: <66F1C864F5E54F80A335D9573FC92F4A@D2HZVNB1>
Content-Type: text/plain; charset="us-ascii"
Gale Clark
gclark@...
+1 508 776 1468
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Sent: Thursday, July 30, 2009 1:03 PM
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Subject: Zbrafish Digest, Vol 50, Issue 6
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Today's Topics:
1. sick fish??? (yin alessa)
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Message: 1
Date: Wed, 29 Jul 2009 19:06:08 -0700 (PDT)
From: yin alessa <yin.alessa@...>
Subject: [Zbrafish] sick fish???
To: bionet-organisms-zebrafish@...
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Dear All,
I had one tank of fish, the males started to get kinky body and become
very short, whereas all the females look very healthy. Does anybody
know what's going on?
Thanks!
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Message: 2
Date: Fri, 31 Jul 2009 14:45:32 -0400
From: "Gale Clark" <gclark@...>
Subject: [Zbrafish] RE: Z Digest, Vol 50, Issue 7 fish with kinky
bodies
To: <zbrafish@...>
Message-ID: <4F2A8D401E134B91AC14E34BAAFE8EBF@D2HZVNB1>
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How many days old are they? How many of the (approximately 12) fish are
affected?
Gale Clark
gclark@...
+1 508 776 1468
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Sent: Friday, July 31, 2009 1:04 PM
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Subject: Zbrafish Digest, Vol 50, Issue 7
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Today's Topics:
1. Luciferase Expression Vector (Kristine Griffett)
2. Re: sick fish??? (yin alessa)
----------------------------------------------------------------------
Message: 1
Date: Thu, 30 Jul 2009 15:34:44 -0400
From: Kristine Griffett <kgriffet@...>
Subject: [Zbrafish] Luciferase Expression Vector
To: zbrafish@...
Message-ID: <95938360-29ED-44CB-B267-1C925EDC5586@...>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
Hello All,
We would like to use a luciferase expression vector to tag cells in
zebrafish embryos and were wondering if anyone has tried to use
vectors commercially available from Promega (pRL-CMV) or New England
BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the
luciferase coding region need to be inserted into a Xenopus or
Zebrafish specific vector for expression of the luciferase in the
embryo?
Kristine Griffett
University of South Florida
Department of Cell Biology, Microbiology and Molecular Biology
4202 E Fowler Ave
BSF 218
Tampa, FL 33620
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Message: 2
Date: Thu, 30 Jul 2009 12:19:56 -0700 (PDT)
From: yin alessa <yin.alessa@...>
Subject: [Zbrafish] Re: sick fish???
To: bionet-organisms-zebrafish@...
Message-ID:
<ea75d5c3-e763-476c-8361-3d396fad9b98@...>
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BTW: the fish were just changed from fry food to adult food. There
are about 6 pairs in the tank, definitely not overcrowded.
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