« Return to Thread: Problem with cultivation on anaerobic solid media

Re: Problem with cultivation on anaerobic solid media

by Mad Twins Dad :: Rate this Message:

Reply to Author | View in Thread


Luzan Tatiana wrote:
In my lab we use hungate bottles only for the liquid anaerobic media and bi-
layer solid media. We have to do inoculations on solid meida only using
Petri dishes in the anaerobic jar supplemented with gas-packs or controlled
atmosphere anaerobic chamber. THere is a problem in the chamber: the cag
mixture we use is 10% Hydrogen,   15% CO2 and Nitrogen as the rest. While
incubating plates inside, the media dries up very quickly and an enourmous
condensation is forming! IT is almost a lake of water there.

IS there any product which would absorb an acess condensate?
Thanks a lot

--

  Tanya Luzan
Research Assistant
Department of Biology
University of Louisiana at Lafayette
Phone (Office): 482-5056

_______________________________________________
Microbio mailing list
Microbio@net.bio.net
http://www.bio.net/biomail/listinfo/microbio
Tanya, I would agree with using sterile selica,
another option you have - just dry your plate for a while.
I guess it will be rather enough to leave 1.5% agar plates overnight.

Cheers.

Sasha.

PS I could not reach your email box, sorry.    

 « Return to Thread: Problem with cultivation on anaerobic solid media