Dr. Erickson lab protocol for get rid of mites (by dechorination):
1) Let flies to lay eggs onto usual vial for overnight or 7 hours.
2) Add 5 ml 50% bleach for 3 min
3) pour out bleach (accurately). Check that eggs are on the surface of the vial.
4) add 10 ml PBS
5) pour out PBS (accurately). Check that eggs are on the surface of the vial
6) add 10 ml PBS
7) pour out PBS (accurately). Check that eggs are on the surface of the vial
8) Let eggs to hatch 37oC 10 days.
Protocol#2 (less efficient)
1) Transform flies every day onto new vial. Repeat 4 times. Grow flies from the 4th vial.
2) Repeat the same with the progeny from the 4th vial.
azza sellami wrote:
hello
please can someone help me to irradicate mites
thanks
