Valeria,
if you haven't loaded your samples yet on the gel, just add 5 or 10ul
2M DTT, it will work fine. If you already have run the gel, don't
panic (too much), you just did not reduce your samples. They will run
at different sizes maybe. You also might see some addional bands due
to cross-linking and inter-/intramolecular -SS- bridges.
Best,
Wo
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