Dear all,
My name is Damiana Ravasi, I am a PhD candidate collaborating with Dr Nichola Illing (Dept of Molecular Biology at UCT) on a project to investigate whether baboons on the Cape Peninsula share the same parasites as humans.
We have successfully amplified the ITS1 and ITS2 regions from genomic DNA from one Trichuris worm isolated from a baboon gut, using the NC2 and NC5 primers (Cutillas et al 2002 Parasitol Res 88: 574-582)...but have not been able to identify the corresponding sequence for Trichuris trichiura from Genbank. We have tried to obtain a human Trichuris trichiura locally, but without success. Next best would be to try and get the sequence of Trichuris trichuira from humans from another source.
Has anyone ever managed to sequence the human Trichuris trichiura ITS1 and ITS2?
Or would anyone have an aliquot of Trichuris trichiura genomic DNA or the nematode specimens which you would willing to send to us?
Thank you very much
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