WIG file display problem

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Parent Message unknown WIG file display problem

by Biliards :: Rate this Message:

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Hello,

I receive this question, but I think that Ryan will find here a
complete answer about his problems..

Alessandra.

---------- Forwarded message ----------
From: Ryan Golhar <golharam@...>
Date: Thu, Oct 22, 2009 at 12:38 AM
Subject: WIG file display problem
To: info@...


Hi,

I've set up a local instance of Gbrowse on my linux server.  It runs
fine with the dataset I've loaded.

Now, I'm trying to upload my own track in the form of a WIG file.
When I try to display it in Gbrowse, I get the following error in my
apache log:


[Wed Oct 21 19:07:09 2009] [error] [client 127.0.0.1] Subroutine
Bio::DB::SeqFeature::Store::berkeleydb::O_RDONLY redefined at
/usr/lib/perl5/5.8.8/Exporter.pm line 65, <F> line 21., referer:
http://localhost:8888/cgi-bin/gbrowse/arabidopsis/
[Wed Oct 21 19:07:09 2009] [error] [client 127.0.0.1]  at
/usr/lib/perl5/site_perl/5.8.8/Bio/DB/SeqFeature/Store/berkeleydb.pm
line 10, referer: http://localhost:8888/cgi-bin/gbrowse/arabidopsis/


I'm not using berkeleydb, I'm using mysql to store my data.  If I
remove the WIG file from being displayed, these errors go away.  Do
you know what I can do to resolve this?



Here's a subset of the wig file I'm using:

browser position chr1:1-47500
browser hide all
browser pack refGene encodeRegions
track type=wiggle_0 name="testwig, chr1 positive strand"
description="testing wig file in gbrowse" visibility=full
color=0,0,255 yLineMark=0 yLineOnOff=on priority=10
variableStep chrom=1 span=1
8429    24
8430    24
8431    24
8432    24
8433    24
8434    24
8435    24
8436    24
8437    24
8438    24
8439    24
8440    24
8441    24



--
 Alessandra Bilardi, Ph. D.
----
 CRIBI, University of Padova, Italy
 Via Ugo Bassi, 58/B - 35121 Padova
 Tel +390498276165 Fax +390498276159
  http://www.linkedin.com/in/bilardi
----
La semplicità è la più alta forma di perfezione (Leonardo Da Vinci)
Keep simple, stupid (KISS, IT)
----

------------------------------------------------------------------------------
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Re: WIG file display problem

by Scott Cain-4 :: Rate this Message:

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Hi Ryan,

I don't think the wiggle file itself is the problem; it is more likely
a configuration problem.  See my comments in the previous email about
debugging and let us know if you still can't figure it out.

Thanks,
Scott


On Thu, Oct 22, 2009 at 7:53 AM, Alessandra
<alessandra.bilardi@...> wrote:

> Hello,
>
> I receive this question, but I think that Ryan will find here a
> complete answer about his problems..
>
> Alessandra.
>
> ---------- Forwarded message ----------
> From: Ryan Golhar <golharam@...>
> Date: Thu, Oct 22, 2009 at 12:38 AM
> Subject: WIG file display problem
> To: info@...
>
>
> Hi,
>
> I've set up a local instance of Gbrowse on my linux server.  It runs
> fine with the dataset I've loaded.
>
> Now, I'm trying to upload my own track in the form of a WIG file.
> When I try to display it in Gbrowse, I get the following error in my
> apache log:
>
>
> [Wed Oct 21 19:07:09 2009] [error] [client 127.0.0.1] Subroutine
> Bio::DB::SeqFeature::Store::berkeleydb::O_RDONLY redefined at
> /usr/lib/perl5/5.8.8/Exporter.pm line 65, <F> line 21., referer:
> http://localhost:8888/cgi-bin/gbrowse/arabidopsis/
> [Wed Oct 21 19:07:09 2009] [error] [client 127.0.0.1]  at
> /usr/lib/perl5/site_perl/5.8.8/Bio/DB/SeqFeature/Store/berkeleydb.pm
> line 10, referer: http://localhost:8888/cgi-bin/gbrowse/arabidopsis/
>
>
> I'm not using berkeleydb, I'm using mysql to store my data.  If I
> remove the WIG file from being displayed, these errors go away.  Do
> you know what I can do to resolve this?
>
>
>
> Here's a subset of the wig file I'm using:
>
> browser position chr1:1-47500
> browser hide all
> browser pack refGene encodeRegions
> track type=wiggle_0 name="testwig, chr1 positive strand"
> description="testing wig file in gbrowse" visibility=full
> color=0,0,255 yLineMark=0 yLineOnOff=on priority=10
> variableStep chrom=1 span=1
> 8429    24
> 8430    24
> 8431    24
> 8432    24
> 8433    24
> 8434    24
> 8435    24
> 8436    24
> 8437    24
> 8438    24
> 8439    24
> 8440    24
> 8441    24
>
>
>
> --
>  Alessandra Bilardi, Ph. D.
> ----
>  CRIBI, University of Padova, Italy
>  Via Ugo Bassi, 58/B - 35121 Padova
>  Tel +390498276165 Fax +390498276159
>  http://www.linkedin.com/in/bilardi
> ----
> La semplicità è la più alta forma di perfezione (Leonardo Da Vinci)
> Keep simple, stupid (KISS, IT)
> ----
>
> ------------------------------------------------------------------------------
> Come build with us! The BlackBerry(R) Developer Conference in SF, CA
> is the only developer event you need to attend this year. Jumpstart your
> developing skills, take BlackBerry mobile applications to market and stay
> ahead of the curve. Join us from November 9 - 12, 2009. Register now!
> http://p.sf.net/sfu/devconference
> _______________________________________________
> Gmod-gbrowse mailing list
> Gmod-gbrowse@...
> https://lists.sourceforge.net/lists/listinfo/gmod-gbrowse
>



--
------------------------------------------------------------------------
Scott Cain, Ph. D.                                   scott at scottcain dot net
GMOD Coordinator (http://gmod.org/)                     216-392-3087
Ontario Institute for Cancer Research

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developing skills, take BlackBerry mobile applications to market and stay
ahead of the curve. Join us from November 9 - 12, 2009. Register now!
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Parent Message unknown Fwd: WIG file display problem

by Vipin TS :: Rate this Message:

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Hi Ryan,

I tried to add the small example mentioned in the previous mail. It is working fine in my local gbrowse instance. Please find the attached image file with this mail.

Here is the configuration stanza

[ShortReadCoverage]
feature      = src
glyph        = wiggle_xyplot
glyph_type   = boxes
min_score    = 0
max_score    = 30
height       = 20
fgcolor      = black
bgcolor      = skyblue
scale        = right
category     = Expression Tracks:Short read coverage experiments
key          = Short Read Coverage Exp 1


Regards,
Vipin T S




------------------------------------------------------------------------------
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ahead of the curve. Join us from November 9 - 12, 2009. Register now!
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wiggle_file.png (73K) Download Attachment

Re: Fwd: WIG file display problem

by Scott Cain-4 :: Rate this Message:

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HI Vipin,

I don't understand your email--is this related to the email that
Alessandra forwarded to the mailing list?

Scott


On Thu, Oct 22, 2009 at 10:01 AM, Vipin TS <vipin.ts@...> wrote:

> Hi Ryan,
>
> I tried to add the small example mentioned in the previous mail. It is
> working fine in my local gbrowse instance. Please find the attached image
> file with this mail.
>
> Here is the configuration stanza
>
> [ShortReadCoverage]
> feature      = src
> glyph        = wiggle_xyplot
> glyph_type   = boxes
> min_score    = 0
> max_score    = 30
> height       = 20
> fgcolor      = black
> bgcolor      = skyblue
> scale        = right
> category     = Expression Tracks:Short read coverage experiments
> key          = Short Read Coverage Exp 1
>
>
> Regards,
> Vipin T S
>
>
>
> ------------------------------------------------------------------------------
> Come build with us! The BlackBerry(R) Developer Conference in SF, CA
> is the only developer event you need to attend this year. Jumpstart your
> developing skills, take BlackBerry mobile applications to market and stay
> ahead of the curve. Join us from November 9 - 12, 2009. Register now!
> http://p.sf.net/sfu/devconference
> _______________________________________________
> Gmod-gbrowse mailing list
> Gmod-gbrowse@...
> https://lists.sourceforge.net/lists/listinfo/gmod-gbrowse
>
>



--
------------------------------------------------------------------------
Scott Cain, Ph. D.                                   scott at scottcain dot net
GMOD Coordinator (http://gmod.org/)                     216-392-3087
Ontario Institute for Cancer Research

------------------------------------------------------------------------------
Come build with us! The BlackBerry(R) Developer Conference in SF, CA
is the only developer event you need to attend this year. Jumpstart your
developing skills, take BlackBerry mobile applications to market and stay
ahead of the curve. Join us from November 9 - 12, 2009. Register now!
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Parent Message unknown Re: WIG file display problem

by Scott Cain-4 :: Rate this Message:

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Vipin,

Please keep replies on the mailing list.

I still don't understand what the relationship is between you and
Ryan.  Can you ask him what version of gbrowse and BioPerl he has and
what he is trying to do?

Scott

On Thursday, October 22, 2009, Scott Cain <scott@...> wrote:

> OK, so in Ryan's initial email, he wrote that he was trying to upload
> a wiggle file; did he mean he was trying to use the GBrowse web
> interface "Upload your own data" to put a wiggle file in GBrowse, or
> was he trying to put a wiggle file into GBrowse for everyone to use?
> Also, what version of GBrowse and BioPerl is he using?
>
> Scott
>
>
> On Thu, Oct 22, 2009 at 10:29 AM, Vipin TS <vipin.ts@...> wrote:
>> Hi Scott,
>>
>> Yes.
>>
>>
>
>
>
> --
> ------------------------------------------------------------------------
> Scott Cain, Ph. D.                                   scott at scottcain dot net
> GMOD Coordinator (http://gmod.org/)                     216-392-3087
> Ontario Institute for Cancer Research
>

--
------------------------------------------------------------------------
Scott Cain, Ph. D.                                   scott at scottcain dot net
GMOD Coordinator (http://gmod.org/)                     216-392-3087
Ontario Institute for Cancer Research

------------------------------------------------------------------------------
Come build with us! The BlackBerry(R) Developer Conference in SF, CA
is the only developer event you need to attend this year. Jumpstart your
developing skills, take BlackBerry mobile applications to market and stay
ahead of the curve. Join us from November 9 - 12, 2009. Register now!
http://p.sf.net/sfu/devconference
_______________________________________________
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Re: WIG file display problem

by Ryan Golhar :: Rate this Message:

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I'm still unable to display my wig file.  I've verified my installation
is clean...this is a new machine I set up just for gbrowse.  All the
other displays seems to work correctly.  I'm still getting this error
when trying to display a wig file.  The display only shows a solid black
line.

Is this how a wig file is supposed to be displayed?  I know the values
are varying in the wig file itself, I would expect to see that in the
display too.

Scott Cain wrote:

> Hi Ryan,
>
> I don't think the wiggle file itself is the problem; it is more likely
> a configuration problem.  See my comments in the previous email about
> debugging and let us know if you still can't figure it out.
>
> Thanks,
> Scott
>
>
> On Thu, Oct 22, 2009 at 7:53 AM, Alessandra
> <alessandra.bilardi@...> wrote:
>> Hello,
>>
>> I receive this question, but I think that Ryan will find here a
>> complete answer about his problems..
>>
>> Alessandra.
>>
>> ---------- Forwarded message ----------
>> From: Ryan Golhar <golharam@...>
>> Date: Thu, Oct 22, 2009 at 12:38 AM
>> Subject: WIG file display problem
>> To: info@...
>>
>>
>> Hi,
>>
>> I've set up a local instance of Gbrowse on my linux server.  It runs
>> fine with the dataset I've loaded.
>>
>> Now, I'm trying to upload my own track in the form of a WIG file.
>> When I try to display it in Gbrowse, I get the following error in my
>> apache log:
>>
>>
>> [Wed Oct 21 19:07:09 2009] [error] [client 127.0.0.1] Subroutine
>> Bio::DB::SeqFeature::Store::berkeleydb::O_RDONLY redefined at
>> /usr/lib/perl5/5.8.8/Exporter.pm line 65, <F> line 21., referer:
>> http://localhost:8888/cgi-bin/gbrowse/arabidopsis/
>> [Wed Oct 21 19:07:09 2009] [error] [client 127.0.0.1]  at
>> /usr/lib/perl5/site_perl/5.8.8/Bio/DB/SeqFeature/Store/berkeleydb.pm
>> line 10, referer: http://localhost:8888/cgi-bin/gbrowse/arabidopsis/
>>
>>
>> I'm not using berkeleydb, I'm using mysql to store my data.  If I
>> remove the WIG file from being displayed, these errors go away.  Do
>> you know what I can do to resolve this?
>>
>>
>>
>> Here's a subset of the wig file I'm using:
>>
>> browser position chr1:1-47500
>> browser hide all
>> browser pack refGene encodeRegions
>> track type=wiggle_0 name="testwig, chr1 positive strand"
>> description="testing wig file in gbrowse" visibility=full
>> color=0,0,255 yLineMark=0 yLineOnOff=on priority=10
>> variableStep chrom=1 span=1
>> 8429    24
>> 8430    24
>> 8431    24
>> 8432    24
>> 8433    24
>> 8434    24
>> 8435    24
>> 8436    24
>> 8437    24
>> 8438    24
>> 8439    24
>> 8440    24
>> 8441    24
>>
>>
>>
>> --
>>  Alessandra Bilardi, Ph. D.
>> ----
>>  CRIBI, University of Padova, Italy
>>  Via Ugo Bassi, 58/B - 35121 Padova
>>  Tel +390498276165 Fax +390498276159
>>  http://www.linkedin.com/in/bilardi
>> ----
>> La semplicità è la più alta forma di perfezione (Leonardo Da Vinci)
>> Keep simple, stupid (KISS, IT)
>> ----
>>
>> ------------------------------------------------------------------------------
>> Come build with us! The BlackBerry(R) Developer Conference in SF, CA
>> is the only developer event you need to attend this year. Jumpstart your
>> developing skills, take BlackBerry mobile applications to market and stay
>> ahead of the curve. Join us from November 9 - 12, 2009. Register now!
>> http://p.sf.net/sfu/devconference
>> _______________________________________________
>> Gmod-gbrowse mailing list
>> Gmod-gbrowse@...
>> https://lists.sourceforge.net/lists/listinfo/gmod-gbrowse
>>
>
>
>


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