cell migration

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cell migration

by Percey :: Rate this Message:

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I am doing cell migration assay using micro glia cells.
Migrated cells are labeled with Calcein.
Can you give me advice on
1, can I use ABI 7300 to read the plates or I have to use a fluorescent plate reader?
2, which company sell phenol red-free MEM?
3, Does CV at 10-20% acceptable for cell migration assay?
Thanks

Percey