Hi,
I am currently working with THP-1 cells (ATCC TIB 202). I mainly work with this cells without differentiating them. I want to do some cell surface expression analysis of these cells using Flow Cytometry, and I am trying to find some good positive controls to perform the necessary compensation in flow cytometry.
Most of the literature I have found on flow cytometry conducted on THP-1 cells is performed on THP-1 cells which have been differentiated with either PMA or Vitamin D. And this differentiation causes a tremendous difference in the expression profile of THP-1 cells, especially CD14 which is not expressed in undifferentiated THP-1 cells.
I am wondering whether anybody can suggest a good marker for undifferentiated THP-1 cells.
Thanks.
Calvin.
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