gbrowse wiggle_xyplot.

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gbrowse wiggle_xyplot.

by vipin.ts :: Rate this Message:

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Hi all,

I would like to represent short read coverage through gbrowse. Here I used the sample input file descried below.

track type=wiggle_0 name=sample
variableStep chrom=I span=10
557 1
variableStep chrom=I span=10
622 1000
variableStep chrom=I span=10
653 2000
variableStep chrom=I span=10
658 3000
variableStep chrom=I span=10
757 4000

Then I made edit in the config file and when I browse through the region of the specified chromosome, the wiggle_xyplot appears.

But the  problem is in the representation of 'y axis' unit in the graph. From the example file I can see that y value is varying from 1 to 4000. but in display I can see only one unit in xy plot. I gave min_score and max_score 0 and 10 respectively in the config file.

It will be great if anyone can help me in the same.
 
Thanks in advance,
Vipin T S


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Re: gbrowse wiggle_xyplot.

by Scott Cain-4 :: Rate this Message:

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Hello Vipin,

Did you sort this out?  I don't know exactly what the problem is, but
I can tell you that your wiggle file doesn't look like the examples on
the gmod.org wiki:

  http://gmod.org/wiki/GBrowse/Uploading_Wiggle_Tracks

and that may cause problems.

Scott


On Mon, Sep 7, 2009 at 4:27 AM, Vipin TS <vipin.ts@...> wrote:

> Hi all,
>
> I would like to represent short read coverage through gbrowse. Here I used
> the sample input file descried below.
>
> track type=wiggle_0 name=sample
> variableStep chrom=I span=10
> 557 1
> variableStep chrom=I span=10
> 622 1000
> variableStep chrom=I span=10
> 653 2000
> variableStep chrom=I span=10
> 658 3000
> variableStep chrom=I span=10
> 757 4000
>
> Then I made edit in the config file and when I browse through the region of
> the specified chromosome, the wiggle_xyplot appears.
>
> But the  problem is in the representation of 'y axis' unit in the graph.
> From the example file I can see that y value is varying from 1 to 4000. but
> in display I can see only one unit in xy plot. I gave min_score and
> max_score 0 and 10 respectively in the config file.
>
> It will be great if anyone can help me in the same.
>
> Thanks in advance,
> Vipin T S
>
>
> ------------------------------------------------------------------------------
> Let Crystal Reports handle the reporting - Free Crystal Reports 2008 30-Day
> trial. Simplify your report design, integration and deployment - and focus
> on
> what you do best, core application coding. Discover what's new with
> Crystal Reports now.  http://p.sf.net/sfu/bobj-july
> _______________________________________________
> Gmod-gbrowse mailing list
> Gmod-gbrowse@...
> https://lists.sourceforge.net/lists/listinfo/gmod-gbrowse
>
>



--
------------------------------------------------------------------------
Scott Cain, Ph. D.                                   scott at scottcain dot net
GMOD Coordinator (http://gmod.org/)                     216-392-3087
Ontario Institute for Cancer Research

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Re: gbrowse wiggle_xyplot.

by vipin.ts :: Rate this Message:

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Hi Scott,

It was problem with my input file.
In my file variableStep chrom=I span=10 stanza was repeating. I found that variableStep support only a fixed span length.
Thanks for your help.

Regards,
Vipin T S

2009/9/16 Scott Cain <scott@...>
Hello Vipin,

Did you sort this out?  I don't know exactly what the problem is, but
I can tell you that your wiggle file doesn't look like the examples on
the gmod.org wiki:

 http://gmod.org/wiki/GBrowse/Uploading_Wiggle_Tracks

and that may cause problems.

Scott


On Mon, Sep 7, 2009 at 4:27 AM, Vipin TS <vipin.ts@...> wrote:
> Hi all,
>
> I would like to represent short read coverage through gbrowse. Here I used
> the sample input file descried below.
>
> track type=wiggle_0 name=sample
> variableStep chrom=I span=10
> 557 1
> variableStep chrom=I span=10
> 622 1000
> variableStep chrom=I span=10
> 653 2000
> variableStep chrom=I span=10
> 658 3000
> variableStep chrom=I span=10
> 757 4000
>
> Then I made edit in the config file and when I browse through the region of
> the specified chromosome, the wiggle_xyplot appears.
>
> But the  problem is in the representation of 'y axis' unit in the graph.
> From the example file I can see that y value is varying from 1 to 4000. but
> in display I can see only one unit in xy plot. I gave min_score and
> max_score 0 and 10 respectively in the config file.
>
> It will be great if anyone can help me in the same.
>
> Thanks in advance,
> Vipin T S
>
>
> ------------------------------------------------------------------------------
> Let Crystal Reports handle the reporting - Free Crystal Reports 2008 30-Day
> trial. Simplify your report design, integration and deployment - and focus
> on
> what you do best, core application coding. Discover what's new with
> Crystal Reports now.  http://p.sf.net/sfu/bobj-july
> _______________________________________________
> Gmod-gbrowse mailing list
> Gmod-gbrowse@...
> https://lists.sourceforge.net/lists/listinfo/gmod-gbrowse
>
>



--
------------------------------------------------------------------------
Scott Cain, Ph. D.                                   scott at scottcain dot net
GMOD Coordinator (http://gmod.org/)                     216-392-3087
Ontario Institute for Cancer Research



--
Vipin T S
Friedrich Miescher Laboratory of the Max Planck Society
Spemannstrasse 39, 72076
Tuebingen, Germany
Tel:+49 7071 601 824

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Re: gbrowse wiggle_xyplot.

by Lincoln Stein-3 :: Rate this Message:

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Hi,

If you can get your data into BAM format, then there is an adaptor in GBrowse that will read the BAM files and directly render coverage tracks on the fly.

Lincoln

On Mon, Sep 7, 2009 at 4:27 AM, Vipin TS <vipin.ts@...> wrote:
Hi all,

I would like to represent short read coverage through gbrowse. Here I used the sample input file descried below.

track type=wiggle_0 name=sample
variableStep chrom=I span=10
557 1
variableStep chrom=I span=10
622 1000
variableStep chrom=I span=10
653 2000
variableStep chrom=I span=10
658 3000
variableStep chrom=I span=10
757 4000

Then I made edit in the config file and when I browse through the region of the specified chromosome, the wiggle_xyplot appears.

But the  problem is in the representation of 'y axis' unit in the graph. From the example file I can see that y value is varying from 1 to 4000. but in display I can see only one unit in xy plot. I gave min_score and max_score 0 and 10 respectively in the config file.

It will be great if anyone can help me in the same.
 
Thanks in advance,
Vipin T S


------------------------------------------------------------------------------
Let Crystal Reports handle the reporting - Free Crystal Reports 2008 30-Day
trial. Simplify your report design, integration and deployment - and focus on
what you do best, core application coding. Discover what's new with
Crystal Reports now.  http://p.sf.net/sfu/bobj-july
_______________________________________________
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--
Lincoln D. Stein
Director, Informatics and Biocomputing Platform
Ontario Institute for Cancer Research
101 College St., Suite 800
Toronto, ON, Canada M5G0A3
416 673-8514
Assistant: Renata Musa <Renata.Musa@...>

------------------------------------------------------------------------------
Come build with us! The BlackBerry® Developer Conference in SF, CA
is the only developer event you need to attend this year. Jumpstart your
developing skills, take BlackBerry mobile applications to market and stay
ahead of the curve. Join us from November 9-12, 2009. Register now!
http://p.sf.net/sfu/devconf
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