Dear all!
I have express soluble protein in origami with pET 32a vector that has Trx (Thioredoxin) tag. But, the problem is aggregation. They are soluble aggregates. In spite of adding NaCl, DTT, EDTA Glycerol etc.( these are the things which are recommended in few papers to overcome aggregation), its still showing aggregation. I cant crystallize the protein or do NMR unless I get rid of this problem. Inspite of aggregation, the protein is active, but probably, it will be more potent if it is in soluble aggregate free form.
I seek advice from all of you over this. Please suggest any possible way out or share your own practical experiences over this.
Thanks
REgards
Shifali
Shifali Chatrath
Graduate Student
Protein science Lab
Dept. of Biological sciences
National University of Singapore
Singapore
+65-65161210
+65-96393449
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