how to prevent aggregation

[Ho Leung Ng]

Have you tried expressing at lower temperature or with much less IPTG?

Try adding ligand/inhibitor with your protein, changing pH, or adding detergent.

The Trx tag may be helping to solubilize your aggregated protein. If
you cut it off, your protein may crash out of solution.

If it's a small protein, you could try refolding.

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