mites

[azza sellami]

hello
please can someone help me to irradicate mites
thanks

[_Elena]

Dr. Erickson lab protocol for get rid of mites (by dechorination):
 
1)      Let flies to lay eggs onto usual vial for overnight or 7 hours.
2)      Add  5 ml 50% bleach for 3 min
3)      pour out bleach (accurately). Check that eggs are on the surface of the vial.
4)      add 10 ml PBS
5)      pour out PBS (accurately). Check that eggs are on the surface of the vial
6)      add 10 ml PBS
7)      pour out PBS (accurately). Check that eggs are on the surface of the vial
8)      Let eggs to hatch 37oC 10 days.
 
Protocol#2 (less efficient)
 
1)      Transform flies every day onto new vial. Repeat 4 times. Grow flies from the 4th vial.
2)       Repeat the same with the progeny from the 4th vial.

azza sellami wrote:

hello
please can someone help me to irradicate mites
thanks

[maki asano]

Hi, you mean "eradicate"?
Pass the flies in vials twice a day for several days and collect embryos
from a several hours incubation, wash them in chlorax, then examine them
for mites under a microscope, transfer (a dozen of eggs are enough) to a
flesh vial and amplify.
 
Good luck,
Maki

Maki Asano, M.D., Ph.D.
Assistant Research Professor

Rm389 CARL Bldg P.O. Box 3657
Department of Molecular Genetics and Microbiology
Duke University Medical Center
Research Dr. Durham,
NC27710, USA

E-mail address: asano@...
Phone: [phone]
Fax:  [phone]
http://mgm.duke.edu/faculty/asano/index.htm

On 12/3/07 10:31 AM, "azza sellami" <a.sellami@...> wrote:

>
> hello
> please can someone help me to irradicate mites
> thanks

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[Kim van der Linde]

Our solution was to wait till the first flies start to emerge from the
pupae, and wash those using a chloride solution. We try to synchronize
the flies within a vial as much as possible, in order to reduce loss of
non-pupated larvae.

Hope this helps.

Kim

azza sellami wrote:
> hello
> please can someone help me to irradicate mites
> thanks

--
http://www.kimvdlinde.com

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[Sumit Gandhi]

On Dec 3, 8:31 pm, azza sellami <a.sell...@...> wrote:
> hello
> please can someone help me to irradicate mites
> thanks
> --
> View this message in context:http://www.nabble.com/mites-tf4937249.html#a14132125
> Sent from the Bio.net - Dros mailing list archive at Nabble.com.

HI,
The easiest solution would be to use Benzyl benzoate. Dilute 1:2 with
methanol and soak cloth or blotting paper in it. Make sure you wear
gloves and mask. Let the cloth or paper dry for a few hours. Keep this
cloth or paper in the trays where you keep your infected vials. Mites
would be gone within a few days.
Regards.

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[marty-26]

On Dec 5, 9:23�pm, Sumit Gandhi <gandhisu...@...> wrote:

> On Dec 3, 8:31 pm, azza sellami <a.sell...@...> wrote:
>
> > hello
> > please can someone help me to irradicate mites
> > thanks
> > --
> > View this message in context:http://www.nabble.com/mites-tf4937249.html#a14132125
> > Sent from the Bio.net - Dros mailing list archive at Nabble.com.
>
> HI,
> The easiest solution would be to use Benzyl benzoate. Dilute 1:2 with
> methanol and soak cloth or blotting paper in it. Make sure you wear
> gloves and mask. Let the cloth or paper dry for a few hours. Keep this
> cloth or paper in the trays where you keep your infected vials. Mites
> would be gone within a few days.
> Regards.

try using anti-mite shelving paper in the future..

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