replica plating

[Frej Tulin]

Dear all
Does anyone have a good method for replica plating Chlamydomonas plates? I
am currently using the standard budding yeast method with a velveteen. I
find that the colonies tend to 'smear out', which makes it difficult to
distinguish individual colonies, especially when the plate has a lot of
colonies.

Is there a filter paper or another material that gives better result?
Many thanks!
/frej
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[Elizabeth Harris]

>Does anyone have a good method for replica plating Chlamydomonas plates?


Whatman #1 filter paper works much better for Chlamy than velveteen.
There's a detailed description of how to go about it, including
photographs, in the first edition (1989) of The Chlamydomonas
Sourcebook, pages 42-47.  A shorter version of the same text appears
in the second edition, volume 1, pages 255-256.


--

Elizabeth H. Harris

Chlamydomonas Center
http://www.chlamy.org/

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