slower growth rate of transformants

[Ye Wang-2]

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Hi, there,

 

Does anybody know the casue of a much more slower growth rate of transformans? we introduced a  5kb heterologous ABC transporter gene in to  Saccharomyces cerevisiae single mutant for a complementation experiment( integration vector, URA3 selection), the transformants did show up on selective medium but stay as a very little tiny colonies even after incubation for up to one week. any trouble shooting tips on this issue?

 

Thanks in advance.

 

Ye

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