Hi all,
I would like to isolate spores of S. cerevisiae from a mixed culture containing spores and vegetative (haploid and some diploid) cells. I obtain that culture by digestion with zymolyase (which, by the way, is rather inefficient at killing vegetative cells of my strain). I am currently using mineral oil and water: spores segregate in the oil and cells segregate in the water. But it is almost impossible to get the spores out of the oil. Even centrifuging at 13,000 rpm for over 20 mins won't transfer them from the oil into water. Ultimately, my goal is to transfer the spores (with almost no vegetative cells) into liquid growth medium. Any suggestions on how I can isolate the spores?
I have checked the archive from the yeast list and I couldn't find a solution to that problem.
Thank you in advance,
Jean-Nicolas
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