stability of luciferase reagents?

View: New views
2 Messages — Rating Filter:   Alert me  

stability of luciferase reagents?

by biochem2 :: Rate this Message:

Reply to Author | View Threaded | Show Only this Message


Hi there,
I am a PhD student in Dortmund/Germany and would like to set up a
luciferase assay- does anyone have experience how to solve the
reagents (luciferase, D-luciferin sodium salt), how stable they are in
solution and if you can freeze stock solutions? And how stable the
bioluminescent signal is?
Thanks a lot in advance!

Uyen

_______________________________________________
Fluorpro mailing list
Fluorpro@...
http://www.bio.net/biomail/listinfo/fluorpro

Re: stability of luciferase reagents?

by piercarlo.montecucchi :: Rate this Message:

Reply to Author | View Threaded | Show Only this Message

You could read the experimental procedures in one of my old papers : "
Stimolazione della formazione di AMP ciclico nel cervello di ratto "in
vitro" da parte della nicergolina" - Il Farmaco 31, 10-17 (1976)

Regards
PCM



_______________________________________________
Fluorpro mailing list
Fluorpro@...
http://www.bio.net/biomail/listinfo/fluorpro