staining of bacterial biofilms

[Rachna Singh]

Why is crystal violet preferred over safranin for staining bacterial
biofilms formed on the surface of microtiter plates?

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[Bob McLean]

Dear Rachna,

While in theory any stain that would stain biological components of
biofilms (and not the underlying polystyrene substratum) could be
used, crystal violet absorbs much more light than safranin and so
would provide higher sensitivity for this assay.

Good luck with your work,

Bob McLean

At 09:15 AM 6/19/2007, Rachna Singh wrote:
>Why is crystal violet preferred over safranin for staining bacterial
>biofilms formed on the surface of microtiter plates?
>
>_______________________________________________
>Biofilms mailing list
>Biofilms@...
>http://www.bio.net/biomail/listinfo/biofilms

R.J.C. (Bob) McLean
Department of Biology
Texas State University
601 University Drive
San Marcos, TX 78666
USA

New University Name: Texas State University

[phone] phone
[phone] FAX
Email: McLean@...

Website: http://www.bio.txstate.edu/~micro/mclean/mclean.html 

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[Rachna Singh]

On Jun 20, 12:48 am, Bob McLean <mcl...@...> wrote:

> Dear Rachna,
>
> While in theory any stain that would stain biological components of
> biofilms (and not the underlying polystyrene substratum) could be
> used, crystal violet absorbs much more light than safranin and so
> would provide higher sensitivity for this assay.
>
> Good luck with your work,
>
> Bob McLean
>
> At 09:15 AM 6/19/2007, Rachna Singh wrote:
>
> >Why is crystal violet preferred over safranin for staining bacterial
> >biofilms formed on the surface of microtiter plates?
>
> >_______________________________________________
> >Biofilms mailing list
> >Biofi...@...
> >http://www.bio.net/biomail/listinfo/biofilms
>
> R.J.C. (Bob) McLean
> Department of Biology
> Texas State University
> 601 University Drive
> San Marcos, TX 78666
> USA
>
> New University Name: Texas State University
>
> [phone] phone
> [phone] FAX
> Email: McL...@...
>
> Website:http://www.bio.txstate.edu/~micro/mclean/mclean.html

OK. Thanks a lot for your help.


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