survey question: container/condition for fish awaiting genotype results

[Timothy Mason-2]

Hi,
I'm gathering information from research labs about the types of
containers used to hold fish awaiting genotyping results.
For example, a fish that undergoes a tail clip will necessarily need to
wait while its genotype is determined via PCR, or, fish that are crossed
to produce embryos used for a phenotype ID will need to wait somewhere
while the embryos develop the phenotype.
Where do you keep the fish?
Do you use static water containers? Which manufacturer? How much water?
Do you have containers that work with your water system to provide fresh
water to the fish while it waits for its result?

Here at the UO research facility we have developed a standard procedure
that allows researchers to keep fish in a plastic container (ZipLoc)
with roughly 32 US ounces (~946 ml) of static water for up to 5 days
while it waits for its result. We are reviewing this procedure and are
hoping to gather information about other best practices for this type of
husbandry procedure.

Thanks, in advance, for any help you can offer.

-Tim

--
Timothy Mason
UO Zebrafish Facility Manager
Eugene OR 97403

phone: [phone]
http://fish.uoregon.edu


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[Claudia-3]

On Aug 24, 12:30 pm, Timothy Mason <tma...@...> wrote:

> Hi,
> I'm gathering information from research labs about the types of
> containers used to hold fish awaiting genotyping results.
> For example, a fish that undergoes a tail clip will necessarily need to
> wait while its genotype is determined via PCR, or, fish that are crossed
> to produce embryos used for a phenotype ID will need to wait somewhere
> while the embryos develop the phenotype.
> Where do you keep the fish?
> Do you use static water containers? Which manufacturer? How much water?
> Do you have containers that work with your water system to provide fresh
> water to the fish while it waits for its result?
>
> Here at the UO research facility we have developed a standard procedure
> that allows researchers to keep fish in a plastic container (ZipLoc)
> with roughly 32 US ounces (~946 ml) of static water for up to 5 days
> while it waits for its result. We are reviewing this procedure and are
> hoping to gather information about other best practices for this type of
> husbandry procedure.
>
> Thanks, in advance, for any help you can offer.
>
> -Tim
>
> --
> Timothy Mason
> UO Zebrafish Facility Manager
> Eugene OR 97403
>
> phone: [phone]http://fish.uoregon.edu

Hi Tim,
I hold one fish in a 1l plastic beaker with lid until results come in.
I cut a hole in the lid to fit a tubing with a pasteur pipette
attached for air supply. I also change the water once a day. If I did
tail clipping I add fungus eliminator to the static system to avoid
infections.

Claudia

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On Aug 24, 10:30 am, Timothy Mason <tma...@...> wrote:

> Hi,
> I'm gathering information from research labs about the types of
> containers used to hold fish awaiting genotyping results.
> For example, a fish that undergoes a tail clip will necessarily need to
> wait while its genotype is determined via PCR, or, fish that are crossed
> to produce embryos used for a phenotype ID will need to wait somewhere
> while the embryos develop the phenotype.
> Where do you keep the fish?
> Do you use static water containers? Which manufacturer? How much water?
> Do you have containers that work with your water system to provide fresh
> water to the fish while it waits for its result?
>
> Here at the UO research facility we have developed a standard procedure
> that allows researchers to keep fish in a plastic container (ZipLoc)
> with roughly 32 US ounces (~946 ml) of static water for up to 5 days
> while it waits for its result. We are reviewing this procedure and are
> hoping to gather information about other best practices for this type of
> husbandry procedure.
>
> Thanks, in advance, for any help you can offer.
>
> -Tim
>
> --
> Timothy Mason
> UO Zebrafish Facility Manager
> Eugene OR 97403
>
> phone: [phone]http://fish.uoregon.edu

Regarding storage of finclipped fish awaiting genotype info:
     We standardly use a stockpile of ~1L plastic aquaria from our old
system.  Fish are stored individually.  We change the water 1-2 days
after fin clip and then every week after that up to 2 weeks.  The
rational is that the fish will foul the water within the first two
days, but without food, a reduced metabolism will dramatically slow
the rate at which they require water changes.  This seems to work well
and we only occasionally lose a fish if we stick to these water
changes.
     Others in the lab isolate finclipped fish in 1L skinny AHAB tanks
on the system, but this uses a lot of shelf space so numbers are
limited.
    Niether of these approaches is ideal, on the system takes up too
much room and off the system takes a considerable amount of time to do
the water changes, especially if doing large numbers.  I've been
trying to think up some better way to handle finclipped fish storage
and thus am very interested in other labs techniques as well.  I would
like to construct a multi-chamber crossing cage style system where 8
or more fish are stored in a row of linked grated bottom pullouts, the
entirety of which are sitting in one large trough of water.  This
would mean a much more simple and quicker water change.  I'm sort of
stumped on what materials to construct this out of.  Has anyone
attempted anything like this?

Regarding test-crossed fish awaiting phenotype info:
  We keep these in their crossing cages without a water change, as we
always know the offspring phenotype by the fifth day.

Gabe Finch
Nicolson Lab
Oregon Health and Sciences University
3181 SW Sam Jackson Pk. Rd. MRB816-L474
Phone:[phone]
Fax:[phone]
finchg.ohsu.edu@...

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[Burdine, Rebecca D]

We seem to be on the lazy side of this.

 

We use plastic containers with lids that we purchase from FIsher Scientific.  We have two sizes, one is 2-3 liters (I think)  the other is more like 1/2 a liter. 

 

We use the large one to hold pairs we are trying to ID. We usually leave a plastic plant in the container.  The smaller ones hold individual fish being evaluated by fin-clip and we do not include a plant.

 

In both cases, we fill the containers about 1/2 to 3/4 full with system water and put the lid on.  I was told it is important to have space for air exchange. 

 

We don't change the water or do anything special until they go back into the system and we often leave our breeding pairs in the larger containers for up to five days.  Fin-clips are turned around in 24 hours.  On occasion, we may leave fish in fin clip containers for two days, but this makes me nervous.

 

We don't see a lot of death with this protocol.  In general, fish that die were either injured during mating or didn't recover from the Tricaine.

 

Becky

 

 

 

---------------------------------------------------

Rebecca D. Burdine, Ph.D.

Assistant Professor

Dept. of Molecular Biology

Princeton University

Washington Road Mof 433

Princeton, NJ 08544 

 

Phone: [phone]

Fax: [phone]

Email: rburdine@...

Admin Assistant: Anna Schmedel [phone]

 

---

From: zbrafish-bounces@... on behalf of finchg@...
Sent: Tue 8/25/2009 2:13 PM
To: bionet-organisms-zebrafish@...
Subject: [Zbrafish] Re: survey question: container/condition for fishawaiting genotype results

On Aug 24, 10:30 am, Timothy Mason <tma...@...> wrote:

> Hi,
> I'm gathering information from research labs about the types of
> containers used to hold fish awaiting genotyping results.
> For example, a fish that undergoes a tail clip will necessarily need to
> wait while its genotype is determined via PCR, or, fish that are crossed
> to produce embryos used for a phenotype ID will need to wait somewhere
> while the embryos develop the phenotype.
> Where do you keep the fish?
> Do you use static water containers? Which manufacturer? How much water?
> Do you have containers that work with your water system to provide fresh
> water to the fish while it waits for its result?
>
> Here at the UO research facility we have developed a standard procedure
> that allows researchers to keep fish in a plastic container (ZipLoc)
> with roughly 32 US ounces (~946 ml) of static water for up to 5 days
> while it waits for its result. We are reviewing this procedure and are
> hoping to gather information about other best practices for this type of
> husbandry procedure.
>
> Thanks, in advance, for any help you can offer.
>
> -Tim
>
> --
> Timothy Mason
> UO Zebrafish Facility Manager
> Eugene OR 97403
>
> phone: [phone]http://fish.uoregon.edu

Regarding storage of finclipped fish awaiting genotype info:
     We standardly use a stockpile of ~1L plastic aquaria from our old
system.  Fish are stored individually.  We change the water 1-2 days
after fin clip and then every week after that up to 2 weeks.  The
rational is that the fish will foul the water within the first two
days, but without food, a reduced metabolism will dramatically slow
the rate at which they require water changes.  This seems to work well
and we only occasionally lose a fish if we stick to these water
changes.
     Others in the lab isolate finclipped fish in 1L skinny AHAB tanks
on the system, but this uses a lot of shelf space so numbers are
limited.
    Niether of these approaches is ideal, on the system takes up too
much room and off the system takes a considerable amount of time to do
the water changes, especially if doing large numbers.  I've been
trying to think up some better way to handle finclipped fish storage
and thus am very interested in other labs techniques as well.  I would
like to construct a multi-chamber crossing cage style system where 8
or more fish are stored in a row of linked grated bottom pullouts, the
entirety of which are sitting in one large trough of water.  This
would mean a much more simple and quicker water change.  I'm sort of
stumped on what materials to construct this out of.  Has anyone
attempted anything like this?

Regarding test-crossed fish awaiting phenotype info:
  We keep these in their crossing cages without a water change, as we
always know the offspring phenotype by the fifth day.

Gabe Finch
Nicolson Lab
Oregon Health and Sciences University
3181 SW Sam Jackson Pk. Rd. MRB816-L474
Phone:[phone]
Fax:[phone]
finchg.ohsu.edu@...

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[Adam Petrie-2]

On Aug 24, 12:30 pm, Timothy Mason <tma...@...> wrote:

> Hi,
> I'm gathering information from research labs about the types of
> containers used to hold fish awaiting genotyping results.
> For example, a fish that undergoes a tail clip will necessarily need to
> wait while its genotype is determined via PCR, or, fish that are crossed
> to produce embryos used for a phenotype ID will need to wait somewhere
> while the embryos develop the phenotype.
> Where do you keep the fish?
> Do you use static water containers? Which manufacturer? How much water?
> Do you have containers that work with your water system to provide fresh
> water to the fish while it waits for its result?
>
> Here at the UO research facility we have developed a standard procedure
> that allows researchers to keep fish in a plastic container (ZipLoc)
> with roughly 32 US ounces (~946 ml) of static water for up to 5 days
> while it waits for its result. We are reviewing this procedure and are
> hoping to gather information about other best practices for this type of
> husbandry procedure.
>
> Thanks, in advance, for any help you can offer.
>
> -Tim
>
> --
> Timothy Mason
> UO Zebrafish Facility Manager
> Eugene OR 97403
>
> phone: [phone]http://fish.uoregon.edu

Most of the time, for holding fish pending ID, the fish are kept in
breeder cages made by Aquatic Habitats.

Depending on which room the fish are housed, it will either be a 2L
breeder cage that is filled about half-full with system water or a 1L
breeder cage filled nearly to the top. We may also keep two fish in
the cages. If so, both fish are from the same breeding/fin clipping,
they are opposite sexes, and a divider is used to keep them separate.
If the fish were spawned in the cages, we will reuse those cages for
the same fish after rinsing them out with system water.

The fish are fed live, freshly hatched Artemia the day they are placed
in the cages and once at mid-day thereafter. The water is exchanged
every other day until the fish are moved back to a fish system. For an
extended period, the cages are replaced and disinfected weekly.

Occasionally, a fish we need to hold separate is already being housed
individually (or with a fish of the opposite sex) in a system tank. In
this case, the fish is returned to its system tank and feeding is as
normal.

Thanks,

Adam Petrie
Laboratory Supervisor, Aquatics
Stowers Institute for Medical Research

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[khelde]

On Aug 24, 12:30 pm, Timothy Mason <tma... from uoregon.edu> wrote:

> Hi,
> I'm gathering information from research labs about the types of
> containers used to hold fish awaiting genotyping results.
> For example, a fish that undergoes a tail clip will necessarily need to
> wait while its genotype is determined via PCR, or, fish that are crossed
> to produce embryos used for a phenotype ID will need to wait somewhere
> while the embryos develop the phenotype.
> Where do you keep the fish?
> Do you use static water containers? Which manufacturer? How much water?
> Do you have containers that work with your water system to provide fresh
> water to the fish while it waits for its result?
>
> Here at the UO research facility we have developed a standard procedure
> that allows researchers to keep fish in a plastic container (ZipLoc)
> with roughly 32 US ounces (~946 ml) of static water for up to 5 days
> while it waits for its result. We are reviewing this procedure and are
> hoping to gather information about other best practices for this type of
> husbandry procedure.
>
> Thanks, in advance, for any help you can offer.
>
> -Tim
>
> --
> Timothy Mason
> UO Zebrafish Facility Manager
> Eugene OR 97403
>
> phone: [phone]http://fish.uoregon.edu

Hi Tim
here in the Moens Lab in Seattle we use small plastic tupperware
containers. They hold 500ml if completely full so we put 300-400ml in
them.  They have 5 holes cut in the lids.  They stack on the bench
such that we have 8 columns wide and 6 columns high (1/2 of a 96-well
plate).  To fill 48 "tanklets" we make up 20 liters of system water
with AmmoLock (2.8ml/20L; Aquarium Pharmaceuticals) and Stress Coat+
Fish and Tap Water conditioner (5.3ml/20L).  We leave the fish in the
tanks, without changing the water, from one to 5 days.  When finished,  
we put the  tanklets through a bleach process and then send them down  
to glassware
for a final washing (water only).
Kathryn Helde
Moens Lab




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