yeast plates with oleic acid

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Parent Message unknown yeast plates with oleic acid

by Stephanie Kaut :: Rate this Message:

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Hello together,

 I want to test some yeast strains for growth on YPAD plates with oleic acid.
 But preparation of the plates seems to be tricky, because oleic acid and
 the agar don't mix well, and the oleic acid swims on the top of the agar.
 What can I do to have nice plates?

Best regards,
Stephanie

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Re: yeast plates with oleic acid

by Frank Dixon :: Rate this Message:

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I have cultured yeast in wall board, and am running remediation tests.
 If I need to isolate yeast species for  Heat, cold envelope testing.
Or isolate for chemical testing or gel shipping purposes, do you
reccommend particular assay to separate cleanly from solid substrate
and identify?  Currently we are using a modified already batched
saccharomeyes w/ecoli xylose breakdown courtesy of DoD operations here
in Santa Ana, Ca.  You guys are the best! thanks Mr. D

On Tue, Jun 23, 2009 at 12:45 PM, Stephanie
Kaut<stephanie.kaut@...> wrote:

> Hello together,
>
>  I want to test some yeast strains for growth on YPAD plates with oleic
> acid.
>  But preparation of the plates seems to be tricky, because oleic acid and
>  the agar don't mix well, and the oleic acid swims on the top of the agar.
>  What can I do to have nice plates?
>
> Best regards,
> Stephanie
>
> _______________________________________________
> Yeast mailing list
> Yeast@...
> http://www.bio.net/biomail/listinfo/yeast
>

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